AI Summary & Key Takeaways
AI InsightPON1 activity and TBARS levels were measured spectrophotometrically; Ox-LDL levels were determined by ELISA; ApoA1levels were determined immunoturbidometrically; and TC, TG, and HDL-C levels were determined enzymatically.Results: In the study we observed that PON1 activity decreases when the number of affected vessels increases (r=-0,537, p
📌 3 Key Takeaways
Abstract
Objective: The aim of this study was to investigate the possible relationship between serum oxidized low-density lipoprotein (Ox-LDL) concentration, recently shown to be a cardiovascular risk factor, paraoxonase 1 (PON1) activity and Thiobarbituric Acid Reactive Substance (TBARS) levels, and the severity of Coronary Artery Disease (CAD) as determined by the obstructive vessel number inpatients. Methods: We determined plasma PON1 activity, serum Ox-LDL concentration, Apolipoprotein A1 (ApoA1) levels, TBARS levels and lipid profiles, including total cholesterol (TC), triglycerides (TG), Low-Density Lipoprotein cholesterol (LDL-C), High-Density Lipoprotein cholesterol (HDL-C), and Very Low-Density Lipoprotein cholesterol (VLDL-C), in patients with CAD (n=42) and in a healthy control group (n=17). The CAD group was divided into three subgroups with single- (n=13), double- (n=15) and triple-vessel (n=14) disease according to their angiography results. PON1 activity and TBARS levels were measured spectrophotometrically; Ox-LDL levels were determined by ELISA; ApoA1levels were determined immunoturbidometrically; and TC, TG, and HDL-C levels were determined enzymatically.Results: In the study we observed that PON1 activity decreases when the number of affected vessels increases (r=-0,537, p
Özet
Amaç: Bu çalışmada koroner arter hastalarında damar tıkanıklığının şiddetine bağlı olarak serum Okside LDL (Ox-LDL) konsantrasyonu; son zamanlarda kardiyovasküler risk faktör olarak tanımlanan Paraoksonaz 1 (PON1) aktivitesi ve tiobarbitürik asit reaktif substansları (TBARS) düzeyleri arasındaki olası ilişki araştırıldı. Yöntemler: Okside LDL, Apolipoprotein AI (Apo AI), TBARS düzeyleri, PON1 aktivitesi lipid profili (Total Kolesterol (TK), Trigliserid (TG), LDL Kolesterol (LDL-K), HDL Kolesterol (HDL-K), VLDL Kolesterol (VLDL-K) düzeyleri 42 koroner arter hastası ve 17 sağlıklı bireyde belirlendi. Hasta grubu anjiografi sonuçlarına göre tek damar (n:13), iki damar (n:15) ve üç damar (n:14) olarak 3 alt gruba bölündü. PONI aktivitesi ve TBARS düzeyleri spektrofotometrik, Ox-LDL düzeyleri Eliza, Apo-AI immunoturbidimetrik, TK, TG ve HDL-K düzeyleri ise enzimatik yöntemlerle tayin edildi. Bulgular: Çalışmamızda damar tıkanıklık derecesi arttıkça PON1 aktivitesinin azaldığı gözlendi (r: -0,537, p