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Journals / İstanbul Kuzey Klinikleri / 2018 / Cilt: 5 - Sayı: 3

Ear atresia: Is there a role of apoptosis-regulating miRNAs?

Emre AKBAŞ
Ezgi ASLAN
Salih YILDIRIM
İbrahim AKALIN
Sarenur YILMAZ
Sena YILMAZ
Ahmet Salih KARAOĞLU
Ubeyde TELLİ
Hilal GÜDEK
Mahmut Tayyar KALCIOĞLU
Pages
238–245
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This isthe first report to present the possible association between apoptosis-regulating miRNAs and ear atresia/microtia.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:OBJECTIVE: The molecular events underlying ear development involve numerous regulatory molecules; however, the roleof microRNAs (miRNAs) has not been explored in patients with ear atresia.
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Key Findings & Data:Here, we aimed to investigate the expressions of20–22 nucleotide noncoding RNAs.METHODS: We selected 12 miRNAs that function to control post-transcriptional gene expression in different pathways, including apoptosis, angiogenesis, and chondrogenesis.
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Conclusion & Impact:This isthe first report to present the possible association between apoptosis-regulating miRNAs and ear atresia/microtia.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: The molecular events underlying ear development involve numerous regulatory molecules; however, the roleof microRNAs (miRNAs) has not been explored in patients with ear atresia. Here, we aimed to investigate the expressions of20–22 nucleotide noncoding RNAs.METHODS: We selected 12 miRNAs that function to control post-transcriptional gene expression in different pathways, including apoptosis, angiogenesis, and chondrogenesis. The altered miRNA expressions were analyzed by real-time PCR fromserum samples of 7 patients with ear atresia and 8 controls.RESULTS: We found that the expression of apoptosis-regulating miRNAs was significantly downregulated in patients with earatresia. TThe expressions of miR126, miR146a, miR222, and miR21 were significantly decreased by 76.2-(p=0.041), 61.8-(p=0.000), 30.5-(p=0.009), and 71.21-fold (p=0.042), respectively, compared with controls.CONCLUSION: Abnormal ear development in ear atresia patients, could possibly be due to the reduced expression of apoptosis regulating miRNAs. Changes in the regulation of tumor protein p53 (TP53), p53 upregulated modulator of apoptosis(PUMA), Fas cell surface death receptor (FAS), FAS ligand (FasL), and phosphatase and tensin homolog (PTEN) directly orwithin the apoptosis-related cascades may play important roles during development, particularly in the external ear. This isthe first report to present the possible association between apoptosis-regulating miRNAs and ear atresia/microtia.

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Cite This Article

AKBAŞ, E., ASLAN, E., YILDIRIM, S., AKALIN, İ., YILMAZ, S., YILMAZ, S., KARAOĞLU, A. S., TELLİ, U., GÜDEK, H., KALCIOĞLU, M. T. (2022). Ear atresia: Is there a role of apoptosis-regulating miRNAs?. İstanbul Kuzey Klinikleri, Cilt: 5 - Sayı: 3, 238–245. https://ajindex.com/articles/551545

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