Journals / İnfeksiyon Dergisi / 2000 / Cilt: 14 - Sayı: 1

The first isolation of Histoplasma capsulatum (Teleomorphic form, Ajellomyces capsulatus) from nature (From wooden fragments in the 89 tumulus, antic city Sardes)

Türkiye'de doğadan (Sardes bintepeler 89 tümülüsü odun buluntularından) ilk kez elde edilen Histoplasma capsulatum (Teleomorfu, Ajellomyces capsulatus) kökeni

Pages
1–14
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This is the first isolation of H. capsulatum from the environment in Turkey.

📌 3 Key Takeaways

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Objective & Hypothesis:A Histoplasma capsulatum strain was isolated from wooden (Juniperus sp, Fraxinus sp) fragments recovered in the excavation carried by Manisa Museum in Bintepeler 89 Tumulus at Sardes antic city in 1989.
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Key Findings & Data:It was converted to the yeast form by incubating several transfers of mycelial culture on BGC agar plates at 37°C on BG to identify them as dimorphic H. capsulatum.
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Conclusion & Impact:This is the first isolation of H. capsulatum from the environment in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

A Histoplasma capsulatum strain was isolated from wooden (Juniperus sp, Fraxinus sp) fragments recovered in the excavation carried by Manisa Museum in Bintepeler 89 Tumulus at Sardes antic city in 1989. Since tuberculate macroconidia resembling those of H. capsulatum were observed, pure isolates were transfered on several media (SDA, PDA, YEA, GLA) to distinguish from Chrysosporium, Renispora flavissima and Sepedonium. It was converted to the yeast form by incubating several transfers of mycelial culture on BGC agar plates at 37°C on BG to identify them as dimorphic H. capsulatum. Sexual (Ajellomyces) state of the fungus was also produced by mating. Macroconidia present in deteriorated tracheids were also observed in wood thin sections. This is the first isolation of H. capsulatum from the environment in Turkey.

Özet

Sardes Bintepeler 89 Tümülüsü'ndeki odun buluntularından alınan örneklerden Histoplasma capsulatum kökeni elde edilmiştir. Saf kültürün SDA, PDA, YEA ve GLA besiyerlerinde geliştirdiği güderi rengindeki kolonilerin yapılan ile mikrokonidi ve makrokonidilerinden dolayı H. capsulatum'un saprofit şeklini düşündüren kökenin, yakın benzerlik gösteren diğer mantarlardan ayırt edilmesi için dimorfizminin gösterilmesi gerekmiş, miselli şekilden maya şekline dönüştüğü, dönüşüm aşamaları da çıplak gözle ve mikroskop altında gözlemlenerek saptanmıştır. Kökenin eşeyli şekli de elde edilmiştir. Köken, Türkiye'de doğadan izole edilen ilk H. capsulatum kökenidir.

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Cite This Article

YÜCEL, A., KANTARCIOĞLU, A. S. (2022). The first isolation of Histoplasma capsulatum (Teleomorphic form, Ajellomyces capsulatus) from nature (From wooden fragments in the 89 tumulus, antic city Sardes). İnfeksiyon Dergisi, Cilt: 14 - Sayı: 1, 1–14. https://ajindex.com/articles/546861

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