Journals / İnfeksiyon Dergisi / 2000 / Cilt: 14 - Sayı: 1

Earlier detection of mycobacteria in clinical specimens by using microcolony method

Mikobakterilerin klinik örneklerde mikrokoloni yöntemi kullanılarak daha hızlı saptanması

Pages
31–36
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The difference between isolation times obtained by two methods was found statistically significant (p

📌 3 Key Takeaways

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Objective & Hypothesis:The purpose of this study was to detect mycobacteria from clinical specimens earlier by using microcolony method compared to the conventional procedure.
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Key Findings & Data:A total of 192 clinical specimens submitted in 1997 were processed and cultured onto both Middlebrook 7H10 agar plates and Lowenstein-Jensen (L-J) agar slants.
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Conclusion & Impact:The difference between isolation times obtained by two methods was found statistically significant (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The purpose of this study was to detect mycobacteria from clinical specimens earlier by using microcolony method compared to the conventional procedure. A total of 192 clinical specimens submitted in 1997 were processed and cultured onto both Middlebrook 7H10 agar plates and Lowenstein-Jensen (L-J) agar slants. The mycobacteria were detected by microscopic examination in 14 of 192 (7.3%) specimens in Middlebrook 7H10 agar plates using microcolony method, whereas in 10 of 192 (5.2%) specimens in L-J slants by direct examination. The average time for mycobacteria isolation was 12.5 days for microcolony method, whereas it was 26.1 days for L-J agar slants for the same isolates. The difference between isolation times obtained by two methods was found statistically significant (p

Özet

Bu çalışmada, mikrokoloni yöntemi kullanılarak mikobakterilerin klinik örneklerden klasik yönteme göre daha erkenden saptanması amaçlanmıştır. Çalışmaya alınan 1997 yılında gönderilmiş toplam 192 klinik örneğin, hem Middlebrook 7H10 agar plaklarına hem de Lowenstein-Jensen (L-J) tüplerine ekimleri yapılmıştır. Middlebrook 7H10 agara ekilen örnekler mikroskop ile incelenmiş ve bu mikrokoloni yöntemiyle pozitiflik %7.3 (14/192), L-J tüplerinde ise doğrudan inceleme ile %5.2 (10/192) olarak saptanmıştır. Basil izolasyon süresinin mikrokoloni yöntemiyle ortalama olarak 12.5 gün, L-J tüplerinde ise 26.1 gün olduğu saptanmıştır. Her iki yöntemle saptanan üreme süreleri arasındaki bu farklılık istatistiksel olarak anlamlı bulunmuştur (p

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Cite This Article

YILDIRAN, Ş. T., SARAÇLI, M. A., AYDOĞAN, H., BAŞUSTAOĞLU, A. C. (2022). Earlier detection of mycobacteria in clinical specimens by using microcolony method. İnfeksiyon Dergisi, Cilt: 14 - Sayı: 1, 31–36. https://ajindex.com/articles/546860

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