SavedAdd your journal

Journals / İnfeksiyon Dergisi / 2005 / Cilt: 19 - Sayı: 3

Detection of HBV-DNA in HBsAg negative blood donors in minipools with two different nucleic acid methods

HBsAg negatif kan vericilerinde küçük havuzlarda HBV-DNA'nın iki farklı nükleik asit yöntemi ile saptanması

Yeşim AYDINOK
Yazan Rüçhan SERTÖZ
Aysu KARADOĞAN
Engin YAYGIN
Selda ERENSOY
Pages
385–388
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The number of HBsAg negative donors has to be increased to show the necessity of HBV DNA screening.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Blood transfusion centers around the world have introduced minipool NAT to reduce the risk of viral pathogens.
📊
Key Findings & Data:This study was undertaken in order to evaluate NAT as a screening test for Hepatitis B Virüs (HBV)2 DNA in blood donations in the Blood, Ege University Hospital.
💡
Conclusion & Impact:The number of HBsAg negative donors has to be increased to show the necessity of HBV DNA screening.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Blood transfusion centers around the world have introduced minipool NAT to reduce the risk of viral pathogens. This study was undertaken in order to evaluate NAT as a screening test for Hepatitis B Virüs (HBV)2 DNA in blood donations in the Blood, Ege University Hospital. HBsAg negative 936 blood donors which filled out the questionairre were included in the study. Minipools containing 24 donor samples were prepared. The pools were centrifuged for one hour at 14000 rpm for virus enrichment. Hepatitis B Virüs DNA was detected with both Cobas Amplicor HBV Test v2.0 and Robogene HBV quantification kit. VQC (Alkmaar, the Netherlands) standard sera containing 30000, 3000, 900, 300 copies/mL were added to minipools as 24th sample before extraction. Cobas Amplicor and Robogene both detected the positivity of pool containing VQC 900 copies/mL, but could not detect VQC 300 copies/mL in minipools. The result of 936 donor samples were negative with both tests. The number of HBsAg negative donors has to be increased to show the necessity of HBV DNA screening.

Özet

Kan transfüzyonlarında infeksiyon etkenlerinin geçişi tüm taramalara rağmen hala ciddi bir problemdir. Dünyada kan merkezlerinde viral etkenlerin geçişini azaltmak için küçük havuzlarda nükleik asit testleri uygulanmaktadır. Bu çalışmada Ege Üniversitesi Hastanesi kan merkezin'de küçük havuzlarda nükleik asit testi ile Hepatit B Virüs (HBV)-DNA taraması amaçlanmıştır. Kan verici sorgulamasından geçmiş 936 HBsAg negatif kan verici çalışmaya alındı. Yirmidört serum örneği içeren küçük havuzlar hazırlandı. Virusları yoğunlaştırmak için havuzlar bir saat 14000 rpm'de santrfüjlendi. Hepatit B Virüs DNA, Cobas Amplicor HBV Test v2.0 (Amplicor, Roche Diagnostics, Branchburg NJ, ABD) ve Robogene HBV kantitasyon kiti (Roboscreen-Real time PCR kit, Almanya) ile tarandı. 30000, 3000, 900, 300 kopya/ml VQC (Alkmaar, Hollanda) standart serumları ekstraksiyon öncesi 24. serum olarak eklendi. Cobas Amplicor ve Robogene her ikisi de VQC 900 kopya/ml içeren küçük havuzu pozitif olarak saptadı ancak VQC 300 kopya/ml'yi saptayamadı. örneklerin 936 Cobas Amplicor ve Robogene HBV kantitasyon kiti ile negatif bulundu. Hepatit B Virüs DNA testinin gerekliliğini ortaya koyabilmeki için taranması gereken HBsAg negatif verici sayısı arttırılmalıdır.

🔖

Cite This Article

AYDINOK, Y., SERTÖZ, Y. R., KARADOĞAN, A., YAYGIN, E., ERENSOY, S. (2022). Detection of HBV-DNA in HBsAg negative blood donors in minipools with two different nucleic acid methods. İnfeksiyon Dergisi, Cilt: 19 - Sayı: 3, 385–388. https://ajindex.com/articles/546471

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Schreiber GB, Busen MP, Kleinman SH, Korelitz JJ. The risk of transfusion-transmitted viral infections. The Retrovirus Epidemiology Donor Study. N EnglJ Med 1996; 334: 1685-90
  2. Weber B, Miihlbacher A, Melchior W. Detection of an acute asymptomatic HBsAg negative hepatitis B virus infection in a blood donor by HBV DNA testing. J Clin Virol 2005; 32: 67-70
  3. Roth WK, Weber M, Seifried E. Feasibility and efficacy of routine PCR screening of blood donations for hepatitis C virus, hepatitis B virus, and HIV-1 in a blood-bank setting. Lancet 1999; 353: 359-63
  4. Biswas R, Tabor E, Hsia CC, et al. Comparative sensitivity of HBV NATs and HBsAg assays for detection of acute HBV infection. Transfusion 2003; 43: 788-98
  5. Otağ F. Destekleyici testler ve moleküler tanı yöntemleri. /. Ulusal Kan Merkezleri ve Transfüzyon Tıbbı Kongresi (24-29 Eylül 2000, Kapadokya) kitabında. 2000: 131-135
  6. Busen MP, Kleinman SH. Nucleic acid amplification testing and disease transmission in Nucleic acid amplification testing of blood donors for transfusion-transmitted infectious diseases. Transfusion 2000; 40: 143-68
  7. Allain JP. Occult hepatitis B virus infection: implications in transfusion. Vox Sanguinis 2004; 86; 83-91
  8. Minegishi K, Yoshikawa A, Kishimoto S, et al. Superiority of minipool nucleic acid amplification technology for hepatitis B virus over chemiluminescence immunoassay for hepatitis B surface antigen screening. Vox Sanguinis 2003; 84: 287-291
  9. Shinichiro S, Ohhashi W, lhara H, et al. Comparison of the sensitivity of NAT using pooled donor samples for HBV and that of a serologic HBsAg assay. Transfusion 2001; 41: 1107-13
  10. Besisik F, Karaca C, Akyuz F, et al. Occult HBV infection and YMDD variants in hemodialysis patients with chronic HCV infection. J Hepatol 2003; 38: 506-10
  11. Kleinman SH, Kuhns MC, Todd DS, et al. Frequency of HBV DNA detection in US blood donors testing positive for the presence of anti-HBc: implications for transfusion transmission and donor screening. Transfusion 2003; 43: 696-704
  12. Gutierrez C, Devesa M, Loureiro CL, et al. Molecular and serological evoiuation of surface antigen negative hepatitis B virus infection in blood donors from Venezuela. J Med Virol 2004; 73:200-7
  13. Offergeld R, Ritter S, Faensen D, Hamouda O. Report of the Robert Koch Institute according to Article 22 of the Transfusion Act for the years 2001 and 2002. Bundes Gesundh 2004; 47: 1216-29.