Journals / IUFS Journal of Biology / 2012 / Cilt: 71 - Sayı: 1

Model bir soğutma kulesi sisteminde polivinil klorid, paslanmaz çelik ve cam yüzeyler üzerindeki biyofilm le ilişkili bakterilerin farklı mikrobiyolojik yöntemlerle incelenmesi

Study of biofilm associated bacteria on polyvinyl chloride, stainless steel and glass surfaces in a model cooling tower system with different microbiological methods

Pages
63–76
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The ATP measurement together with fluorescence staining reflected the changes in the biofilms more distinctly than the HPC.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Cooling towers are an integral part of large range of industrial processes.
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Key Findings & Data:In this study, bulk water and biofilm associated microbial load were analyzed in terms of heterotrophic plate count (HPC), epifluorescence microscopy, carbohydrate quantity, total and free ATP concentration and compared material dependence.
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Conclusion & Impact:The ATP measurement together with fluorescence staining reflected the changes in the biofilms more distinctly than the HPC.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Cooling towers are an integral part of large range of industrial processes. The conditions of cooling water systems provide an ideal environment for microbial growth and biofilm formation. Microorganisms which enter via makeup water or air to the system form a biofilm layer on the inside surfaces of cooling tower which contain water and limited nutrients. Biofilm formation in cooling tower systems is undesirable for operational and public health reasons. Thus, microbial load should be monitored and kept under control. In this study, bulk water and biofilm associated microbial load were analyzed in terms of heterotrophic plate count (HPC), epifluorescence microscopy, carbohydrate quantity, total and free ATP concentration and compared material dependence. Kruskal-Wallis statistical analysis revealed that there were not significant differences between the tested slide materials according to HPC, DAPI-CTC staining, and ATP measurement. The ATP measurement together with fluorescence staining reflected the changes in the biofilms more distinctly than the HPC.

Özet

Soğutma kuleleri birçok endüstriyel sürecin parçasıdır. Soğutma suyu sistemindeki koşullar mıkrobiyal gelişim ve biyofilm oluşumu için ideal bir ortam sağlar. Tamamlama suyu veya havadan sisteme giren mikroorganizmalar su ve kısıtlı besin içeren soğutma kulesinin ıç yüzeylerinde biyofilm tabakası oluştururlar. Soğutma kulesi sistemlerinde biyofilm oluşumu, sistemin çalışma koşulları ve kamu sağlığı nedenleri ile istenmeyen bir durumdur. Bu nedenle mikrobiyal yük takip edilmeli ve kontrol altında tutulmalıdır. Çalışmada su ve biyofilmle ilişkili mikrobiyal yük heterotrofik plak sayım, epıfloresan mikroskobi, karbonhidrat miktarı, toplam ve serbest ATP konsantrasyonu açısından incelenmiş ve materyaller kıyaslaıımıştır. Kruskal-Wallis istatistik analizine göre test edilen malzemeler arasında heterotrofik plak sayını, epifloresan mikroskobi. toplam ve serbest ATP konsantrasyonu açısından önemli bir fark tespit edilmemiştir. Floresan boyama ve ATP ölçümü tekniklerinin birlikte kullanımı biyofılmdeki değişiklikleri heterotrofik plak sayımdan daha net yansıtmaktadır.

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Cite This Article

SANLI, N. O. Y. (2022). Model bir soğutma kulesi sisteminde polivinil klorid, paslanmaz çelik ve cam yüzeyler üzerindeki biyofilm le ilişkili bakterilerin farklı mikrobiyolojik yöntemlerle incelenmesi. IUFS Journal of Biology, Cilt: 71 - Sayı: 1, 63–76. https://ajindex.com/articles/544030

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