Diyabetik albino Balb/ c farelerdea aminoguanidinin böbrek üzerindeki etkisi

Objective & Hypothesis:The aim of this study is to find out how activated inducible nitric oxide synthase (iNOS) and nitric oxide (NO) affect kidney tissue in streptozotocin (STZ)-induced diabetic mice and whether its influence can be prevented by aminoguanidine (AG), a specific iNOS inhibitor.

📊

Key Findings & Data:Twenty-four male mice were divided into four study groups (n=6) receiving a daily dose of 100 mg.kg-1 AG for 90 days (Group AG), a single dose of 150 mg.kg-1 STZ (Group STZ), a single dose of 150 mg.kg-1 STZ followed by daily administration of 100 mg.kg-1 AG for 90 days (Group STZ-AG), and intraperitoneally injections of saline only (Group Control) for 90 days.

💡

Conclusion & Impact:There is a possible relationship between increased iNOS and dispersion of renin granules in juxtaglomerular cells in diabetes.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

The aim of this study is to find out how activated inducible nitric oxide synthase (iNOS) and nitric oxide (NO) affect kidney tissue in streptozotocin (STZ)-induced diabetic mice and whether its influence can be prevented by aminoguanidine (AG), a specific iNOS inhibitor. Twenty-four male mice were divided into four study groups (n=6) receiving a daily dose of 100 mg.kg-1 AG for 90 days (Group AG), a single dose of 150 mg.kg-1 STZ (Group STZ), a single dose of 150 mg.kg-1 STZ followed by daily administration of 100 mg.kg-1 AG for 90 days (Group STZ-AG), and intraperitoneally injections of saline only (Group Control) for 90 days. Dispersion of NADPH-diaphorase (NADPH-d) was stronger in the kidney sections of STZ-treated animals compared with the controls. STZ treatment caused disruption of continuity of the brush borders in proximal tubules, glomerular endothelial damage, and considerable renin granules in the juxtaglomerular cells. AG administration following STZ treatment partly prevented histological and cytological changes in kidney cortex, and renin dispersion was similar to that in control animals. We found that increased inducible nitric oxide (iNO) caused kidney tissue degeneration that could be prevented to some extent by AG treatment. There is a possible relationship between increased iNOS and dispersion of renin granules in juxtaglomerular cells in diabetes.

Özet

Bu çalışmanın amacı, uyarılabilir nitrik oksit sentaz (iNOS) aktivasyonunun ve nitrik oksidin streptozotos in (STZ) ile uyarılmış diyabetik farelerde böbrek dokusunu nasıl etkilediğini ve etkisinin spesifik bir iNOS inhibitörü olan a minoguanidin (AG) ile önlenip önlenemediğini öğrenmektir. Yirmi dört erkek fare, 90 gün boyunca günlük 100 mg. kg - 1 AG (AG Grubu), tek doz 150 mg. kg- 1 STZ (STZ Grubu),tek doz 150 mg. kg - 1 STZyi takiben 90 gün boyunca günlük 100 mg. kg - 1 AG (STZ - AG Grubu) ve sadece intraperitonal fizyolojik tuzlu su (Kontrol Grubu) alan dört gruba ayrılmıştır. NADPH - diaforez (NADPH -d) dağılımı, STZ uygulanmış hayvanların böbrek kesitlerinde kontrolle karşılaştırıldığında daha fazlaydı. STZ uygulaması proksimal tübüllerde fırç a kenarların devamlılığının bozulmasına, glomerulus endotelinde hasara ve juks taglomerular hücrelerde renin granüllerinin daha fazla olmasına yol açmıştır . STZ uygulamasını takiben verilen AG, böbrek korteksindeki histolojik ve sitolojik değişiklikleri kıs men önlemiştir ve renin dağılımı kontrol hayvanlardakine benzer şekilde olmuştur. Uyarılabilir nitrik oksit (iNO) artışıyla böbrekte meydana gelen bozulmanın AG uygulamasıyla kısmen önlenebildiği bulunmuştur. Diyabette, artan iNOS ile jukstaglomerular hücr elerde reningranülleri dağılımı arasında olası bir ilişki vardır

🔖

Cite This Article

YILMAZER, N., GUREL, E., TANSEL, C. D. (2022). Diyabetik albino Balb/ c farelerdea aminoguanidinin böbrek üzerindeki etkisi. IUFS Journal of Biology, Cilt: 71 - Sayı: 1, 17–29. https://ajindex.com/articles/544021

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (12)

Atasayar S., Gürer-Orhan H., Orhan H., Gürel B., Girgin G. and Ozgüneş H. (2009) Preventive effect of aminoguanidine compared to vitamin E and C on cisplatin-induced nephrotoxicity in rats. Exp. Toxicol. Pathol61 (1): 23-32.
Azal O., Yönem A., Güler S., Cakir B., Baydar A., Corakçi A. and Kutlu M. (2002) Effects of aıninoguanidıne and tolrestat on the development of ocular and renal structural changes in experimental diabetic rats. Diabetes Obes. Metab., 4(1): 75-79.
Bachmann S., Bosse H.M. and Mundel P. (1995) Topography of nitric oxide synthesis by localizing constitutive NO synthases in mammalian kidney. Am. J. Physiol., 268: F885-F898. Birrell A.M., Heffeman S.J., Kirwan P., McLennan S., Gillin A.G. and Yue D.K. (2002) The effects of aminoguanidine on renal changes in a baboon model of Type 1 diabetes. J. Diabetes Complications, 16 (4): 301-309.
Bogdan C., Rollinghoff M. and Diefenbach, A. (2000) The role of nitric oxide in innate immunity. Immunol. Re>.y 173: 17-26.
Bogdan C. (2001) Nitric oxide and the immune response. Nat. Immunol., 2 (10): 907-916.
Brown G.C. (1999) Nitric oxide and mitochondrial respiration. Biochim. Biophys. Acta, 1411 (2-3): 351-369.
Bucher M., Ittner K.P., Hobbhahn J., Taeger K. and Kurtz A. (2001) Downregulation of angiotensin II type 1 receptors during sepsis. Hypertension, 38 (2): 177-182.
De Vriese A.S., Stoenoiu M.S., Elger M., Devuyst O., Vanholder R., Kriz W. and Lameire N.H. (2001) Diabetes-induced microvascular dysfunction in the hydronephrotic kidney: Role of nitric oxide. Kidney Int., 60 (1): 202-210.
Ellis E.N. and Good B.H. (1991) Prevention of glomerular basement membrane thickening by aminoguanidine in experimental diabetes mellitus. Metabolism, 40(10): 1016-1019.
Forstermann U., Closs E.L., Pollock J.S., Nakane M., Schwarz P., Gath I. and Kleınert H. (1994) Nitric oxide synthase isozymes. Characterization,purification, molecular cloning, and functions. Hypertension, 23: 1121-1131.
Fujii H., Nakamura S., Kuroda S., Yoshihara F., Nakahama H., Inenaga T., Ueda-Ishibashi H., Yutani C. and Kawano Y. (2006) Relationship between renal artery stenosis and intrarenal damage in autopsy subjects with stroke. Nephrol Dial Transplant., 21 (1): 113-119.
Furusu A., Miyazaki M., Abe K, Tsukasaki S., Shioshita K., Sasaki O., Miyazaki K., Ozono Y., Koji T., Harada T., Sakaı H. and Kohno S. (1998) Expression of endothelial and inducible nitric oxide synthase in human glomerulonephritis. Kidney Int., 53 (6): 1760-1768. Heidland A.,