Journals / IUFS Journal of Biology / 2013 / Cilt: 72 - Sayı: 2

Aljinat boncukları ile trametes versicolor lakkazının optimum immobilizasyon koşullarının belirlenmesi

Determination of optimum immobilization conditions of trametes versicolor laccase with sodium alginate beads

Pages
15–21
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Maximumlaccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200mg of sodium alginate amount.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Laccase (EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmentalpollutants such as phenolic compounds.
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Key Findings & Data:The efficiency of the enzyme for environmental and industrialapplications can be increased by immobilizing the enzyme on a carrier.
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Conclusion & Impact:Maximumlaccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200mg of sodium alginate amount.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Laccase (EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmentalpollutants such as phenolic compounds. The efficiency of the enzyme for environmental and industrialapplications can be increased by immobilizing the enzyme on a carrier. In the present study, laccaseobtained from Trametes versicolor (fungi) was immobilized on sodium alginate beads and kappa-carrageenan and the effect of contact time, pH, temperature, the amount of carrier and enzymeconcentration were investigated to determine optimum conditions of laccase immobilization. Sodiumalginate beads were chosen as the most efficient carrier due to their high immobilization yield. Maximumlaccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200mg of sodium alginate amount.

Özet

Lakkaz (EC 1.10.3.2), fenolik bileşikler gibi çeşitli çevresel kirleticilerin oksidasyonunu katalizleyençoklu bakır enzimidir. Çevresel ve endüstriyel uygulamalar için bir taşıyıcı üzerinde enzimin immobilizeedilmesiyle etkinliği arttırılabilir. Bu çalışmada, Trametes versicolordan elde edilen lakkaz sodiumaljinat ve kappa-karagen üzerinde immobilize edilmiş, temas süresi, pH, sıcaklık, taşıyıcı miktarı, enzimkonsantrasyonun etkisi değerlendirilmiştir. Sodyum aljinat ve kappa-karragenanın immobilizasyonetkinliği araştırılmıştır. Sonuç olarak, sodyum aljinat boncukları yüksek immobilizasyon verimindendolayı en iyi taşıyıcı olarak seçilmiştir. Maksimum lakkaz immobilizasyonu, 30 dakika temas süresi, pH4.5, 30 °C, 200 mg sodyum aljinat koşullarında elde edilmiştir.

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Cite This Article

ÜNAL, A., KOLANKAYA, N. (2022). Aljinat boncukları ile trametes versicolor lakkazının optimum immobilizasyon koşullarının belirlenmesi. IUFS Journal of Biology, Cilt: 72 - Sayı: 2, 15–21. https://ajindex.com/articles/544018

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