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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2022 / Cilt: 12 - Sayı: 2

Evaluation of Some Phenolic Compounds as Inhibitors of Glutatione S-Transferase Enzyme

Bazı Fenolik Bileşiklerin Glutatyon S-Transferaz Enzim İnhibitörleri Olarak Değerlendirilmesi

Muhammet Serhat ÖZASLAN
Pages
882–889
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to these results, esculetin hydrate showed the best inhibition effect and competitive inhibition.

📌 3 Key Takeaways

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Objective & Hypothesis:Glutathione S-transferases (GSTs) are important antioxidant enzyme and catalyze the conjugation of glutathione with toxic metabolites.
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Key Findings & Data:The IC50 values of morine hydrate, esculetin hydrate, p-coumaric acid and syringaldehyde phenolic compounds are found as 0.718 μM, 0.713 μM, 0.701 μM, and 0.699 μM, respectively.
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Conclusion & Impact:According to these results, esculetin hydrate showed the best inhibition effect and competitive inhibition.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Glutatyon S-transferaz · inhibisyon · fenolik bileşik

Abstract

Glutathione S-transferases (GSTs) are important antioxidant enzyme and catalyze the conjugation of glutathione with toxic metabolites. In this study, potential inhibitory effects of some phenolic compounds on the activity of GST enzyme were tested in vitro. The IC50 values of morine hydrate, esculetin hydrate, p-coumaric acid and syringaldehyde phenolic compounds are found as 0.718 μM, 0.713 μM, 0.701 μM, and 0.699 μM, respectively. Ki values are found as 1.610±0.120, 0.179±0.019, 4.590±0.480, 0.999±0.070, respectively. According to these results, esculetin hydrate showed the best inhibition effect and competitive inhibition.

Özet

Glutatyon S-transferazlar (GSTs) önemli antioksidan enzim sınıfındadırlar ve glutatyonun toksik metabolitlerle konjugasyonunu katalize ederler. Yapılan bu çalışmada, bazı fenolik bileşiklerin GST enziminin aktivitesi üzerine potansiyel inhibisyon etkileri in vitro olarak test edilmiştir. Morin hidrat, eskuletin hidrat, p-kumarik asit ve siringaldehit fenolik bileşiklerinin GST enzimi üzerine IC50 değerleri sırasıyla 0.718 μM, 0.713 μM, 0.701 μM ve 0.699 μM olarak bulunmuştur. Ki değerleri ise sırasıyla 1.610±0.120, 0.179± 0.019, 4.590±0.480, 0.999±0.070 olarak bulunmuştur. Bu sonuçlara göre en iyi inhibisyon etkisini eskuletin hidrat göstermiş olup yarışmalı inhibisyon türü sergilemiştir.

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Cite This Article

ÖZASLAN, M. S. (2022). Evaluation of Some Phenolic Compounds as Inhibitors of Glutatione S-Transferase Enzyme. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 12 - Sayı: 2, 882–889. https://ajindex.com/articles/538418

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