SavedAdd your journal

Journals / Hayvansal Üretim / 2015 / Cilt: 56 - Sayı: 2

Phylogenetic Analysis of Anaerobic Fungal Isolates by Randomly Amplified Polymorphic DNA (RAPD)

Anaerobik Rumen Fungus İzolelerinin Rastgele Çoğaltılmış Polimorfik DNA (RAPD) Metodu ile Filogenetik Analizi

Bulent KAR
Ismail AKYOL
Ugur COMLEKCİOGLU
Mehmet Sait EKİNCİ
Emin OZKOSE
Pages
14–21
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

A remarkable polymorphism was detected to allow the separation of individuals and all the 18 fungal isolates were clearly distinguished from each other

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Phylogenetic analysis was conducted to asses the relationships between the isolates from the various genera of anaerobic rumen fungi.
📊
Key Findings & Data:A set of 18 fungal cultures isolated and purified from the ruminant herbivores from different geographic locations of Turkey was analyzed with the aid of randomly amplified polymorphic DNA (RAPD) technique.
💡
Conclusion & Impact:A remarkable polymorphism was detected to allow the separation of individuals and all the 18 fungal isolates were clearly distinguished from each other
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Phylogenetic analysis was conducted to asses the relationships between the isolates from the various genera of anaerobic rumen fungi. A set of 18 fungal cultures isolated and purified from the ruminant herbivores from different geographic locations of Turkey was analyzed with the aid of randomly amplified polymorphic DNA (RAPD) technique. Polymerase chain reaction (PCR) using 10 arbitrary decamer oligonucleotide primers applied to the 18 anaerobic fungal isolates. A Total of 64 different marker bands were observed and 92.18% of these bands were found to be polymorphic. The sizes of the amplified DNAs were mainly range between 100 bp and 700 bp. A remarkable polymorphism was detected to allow the separation of individuals and all the 18 fungal isolates were clearly distinguished from each other

Özet

Bu çalışmada farklı cinslere ait anerobik rumen fungus izolatları arasındaki ilişkinin ortaya konması amacıyla filogenetik analiz, yapılmıştır. Türkiye'nin farklı coğrafi bölgelerinden izole edilen onsekiz fungus kültürü Rastgele Çoğaltılmış Polimorfik DNA (RAPD) tekniği ile analiz edilmiştir. İzole edilen onsekiz fungus kültürüne 10 baz uzunluğundaki primerler kullanılarak Polimeraz Zincir Reaksiyonu (PZR) uygulanmıştır. Toplam olarak 64 markır bandı gözlemlenmiş ve bu bantların %92.18'inin polimorfik olduğu saptanmıştır. Çoğaltılan DNA büyüklükleri 100 bç ile 700 bç arasında değişmektedir. Fungusları birbirinden ayrıt etmek için önemli derecede polimorfizim gözlemlenmiş ve izole edilen 18 fungusun tamamı açık bir şekilde birbirinden ayrılmıştır

🔖

Cite This Article

KAR, B., AKYOL, I., COMLEKCİOGLU, U., EKİNCİ, M. S., OZKOSE, E. (2022). Phylogenetic Analysis of Anaerobic Fungal Isolates by Randomly Amplified Polymorphic DNA (RAPD). Hayvansal Üretim, Cilt: 56 - Sayı: 2, 14–21. https://ajindex.com/articles/536178

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Barr, DJS, Yanke LJ., Bae, HD, McAllister, TA., Cheng, KJ. 1995. Contributions on the morphology and taxonomy of some rumen fungi from Canada. Mycotaxon LIV: 203-214.
  2. Billon-Grand, G., Fiol, JB., Breton, A., Bruyere, A., Oulhaj, Z. 1991. DNA of some anaerobic rumen fungi: G + C content determination. FEMS Microbiol. Lett. 82: 267-270.
  3. Breton, A., Bernalier, A., Bonnemoy, F., Fonty, G., Gaillard, B. Gouet PH. 1989. Morphological and metabolic characterization of a new species of strictly anaerobic rumen fungus: Naocallimastix joyonii. FEMS Microbiol. Lett. 58: 309-314.
  4. Brookman, J.L., Mennim, G., Trinci, A.P.J., Theodorou, M.K., Tuckwell, D.S. 2000. Identification and characterization of anaerobic fungi using molecular methodologies based on ribosomal ITS1 and 18S rRNA. Microbiol. 146: 393-403.
  5. Brownlee, A.G. 1994. The nucleic acids of anaerobic fungi. In: Mountfort D.O., Orpin C.G., editors. Anaerobic fungi biology, ecology and function. Marcel Dekker, New York, pp 241-256.
  6. Callaghan, T.M., Podmirseg, S.M., Hohlweck, D., Edwards, J.E., Puniya, A.K., Dagar, S.S. and Griffith, G.W. 2015. Buwchfawromyces eastonii gen nov., (Neocallimastigomycota) isolated from buffalo faeces. MycoKeys 9: 11-28. anaerobic fungus Coetzee, M.P.A., Wingfield, B.D., Bloomer, P., Ridley, G.S., Kile, G.A., Wingfiel, M.J. 2001. Phylogenetic relationships of Australian and New Zealand Armillaria species. Mycologia 93: 887-896.
  7. Davies, D.R., Theodorou, M.K., Lawrence, M.I., Trinci, A.P.J. 1993. Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces J. Gen. Microbiol. 139: 1395-1400.
  8. Doherty, K.R., Zweifel, E.W., Elde, N.C., Mckone, M.J., Zweifel, S.G. 2003. Random amplified polymorphic DNA markers reveal genetic variation in the symbiotic fungus of leaf-cutting ants. Mycologia 95: 19-23.
  9. Dore, J., Stahl, D.A. 1991. Phylogeny of anaerobic rumen Chytridiomycetes inferred from small subunit ribosomal RNA sequence comparison. Can. J. Bot. 69: 1964-1971.
  10. Fliegerova, K., Hodrova, B., Voigt, K. 2004. Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi. Folia Microbiol. 49: 157-164. Góes, L.B., Costa, A.B.L., Freire, L.L.C., Oliveira, N.T. 2002. Randomly amplified polymorphic DNA of Trichoderma isolates and antagonism against Rhizoctonia solani. Brazi Arch. of Biol. Technol. 45: 151-160.
  11. Gold, J.J., Heath, I.B., Bauchop, T. 1988. Ultrastructural description of a new chytrid genus of caecum anaerobe, Ca