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Journals / Harran Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 3 - Sayı: 2

Identification and Phage Susceptibilities of Brusella Strains In Turkey Between the Years 2007 and 2008

Türkiye'de 2007 ve 2008 Yılları Arasında İzole Edilen Brusella Suşlarının İdentifikasyonu ve Faj Duyarlılıklarının Saptanması

Sevil ERDENLİĞ GÜRBİLEK
Hüseyin Yavuz AKSOY
Emin Ayhan BAKLAN
Pages
67–72
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that 96% of cattle isolates was B. abortus biotype 3 and 4% was B.melitensis.

📌 3 Key Takeaways

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Objective & Hypothesis:A total of 114 Brucella isolates consisting of 43 cattle, 61 sheep and goats, 8 human and 2 food origins were identified by conventional biotyping procedures.
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Key Findings & Data:The results showed that 96% of cattle isolates was B. abortus biotype 3 and 4% was B.melitensis.
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Conclusion & Impact:Forty seven (67.2%), 15 (21.4%) and 8 (11.4%) out of 70 B. melitensis isolates conformed to phage typing pattern C, D and B, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

A total of 114 Brucella isolates consisting of 43 cattle, 61 sheep and goats, 8 human and 2 food origins were identified by conventional biotyping procedures. The results showed that 96% of cattle isolates was B. abortus biotype 3 and 4% was B.melitensis. One of B. melitensis was B.melitensis biotype 3 and the other was B. melitensis biotype 1. Of sheep and goat isolates, 65.6 % was B. melitensis biotype 3 and 29.5% was B. melitensis biotype 1. Three of the sheep strains (4.9 %) were identified as B. abortus biotype 3. None of the test strains was identified as B.abortus S19 ve B.melitensis Rev.1 vaccine strains. Seven out of 8 strains from humans were identified as B. melitensis biotype 3. One strain of human origin was lysed completely by R/C phage. This isolate was not agglutinated by anti M and anti A monospecific sera and was agglutinated by both R antisera and acriflavin displaying rough characteristics. Two of the strains from food (ice cream) were B. melitensis biotype 1 and B. melitensis biotype 3. Four of B. melitensis strains were are atypically susceptible to penicilin and 2 of the B. melitensis strains were evaluated as atypical regarding to their susceptibility to basic fuhsin (20 µg/ml). All the B.melitensis strains were tested for the susceptibility to the phages of Tbilisi, Berkeley, Weybridge, Izatnagar and R/C at routine test dilutions (RTD). Forty seven (67.2%), 15 (21.4%) and 8 (11.4%) out of 70 B. melitensis isolates conformed to phage typing pattern C, D and B, respectively.

Özet

Çalışmada 43 sığır, 61 koyun, 8 insan ve 2 adet gıda orijinli olmak üzere toplam 114 brusella suşu konvensiyonel biyotiplendirme prosedürü ile biyotiplendirildi. Sığır kökenli suşların % 96'sı B.abortus biyotip 3 ve % 4'ü B.melitensis olarak identifiye edildi. B.melitensis suşlarının biri B.melitensis biyotip 3 ve diğeri B.melitensis biyotip 1 idi. Koyun ve keçi kökenli suşların % 65.6'sı B.melitensis biyotip 3 ve % 29.5'u B.melitensis biyotip 1 olarak identifiye edildi. Koyun suşlarından 3'ü (%4.9) ise B.abortus biyotip 3 olarak tespit edildi. Test suşlarından hiçbiri B.abortus S19 ve B.melitensis Rev.1 aşı suşları olarak saptanmadı. İnsanlardan izole edilen 8 suşun 7'si B.melitensis biyotip 3 olarak ve dondurmadan izole edilen suşlardan biri B.melitensis biyotip 3 ve diğeri B.melitensis biyotip 1 olarak idendifiye edildiler. İnsandan izole edilen suşlardan birinin rough özellikte ve R/C fajı ile tamamen lize olduğu tespit edildi. B.melitensis suşlarından 4'ü atipik olarak penisiline duyarlılık gösterirken, 2'si bazik fuksine (20 µg/ml) gösterdiği duyarlılık nedeni ile atipik olarak değerlendirildi. Tüm B.melitensis suşları rutin test dilüsyonundaki (RTD) Tbilisi, Berkeley, Weybridge, Izatnagar ve R/C fajlarına duyarlılık açısından test edildiler. Toplam 70 B.melitensis suşunun 47'si (%67.2) C, 15'i (%21.4) D ve 8'i (%11.4) B faj tip kalıbına uyan reaksiyonlar gösterdi.

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Cite This Article

GÜRBİLEK, S. E., AKSOY, H. Y., BAKLAN, E. A. (2022). Identification and Phage Susceptibilities of Brusella Strains In Turkey Between the Years 2007 and 2008. Harran Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 3 - Sayı: 2, 67–72. https://ajindex.com/articles/534404

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References (11)

  1. Aldomy FMM, Jahans KL, and Altarzi YH, 1992: Isolation of Brucella melitensis from aborting ruminants in Jordan. J Comp Path, 107,239-242.
  2. Alton GG, Jones LM, Angus RD, Verger JM, 1988:Bacteriological methods. In: "Techniques the Brucellosis Laboratory". INRA, pp 13-61. Anonim, 1986: Joint FAO/WHO, Expert Committee on Brucellosis, Geneva WHO/Techical Report, 6th. Report Series No:740. Bolca Z, Gündeş S, Erdenliğ S, Öztürk R, Sümerkan B, Akata F. ve Vahaboğlu H, 2002: İnsan kaynaklı Brusella türü mikroorganizmaların tiplendirilmesi amacı ile uygulanan metotların karşılaştırılması ve biyotipleri ile faj tipleri arasındaki ilişkinin irdelenmesi. Flora, 7(3),157-170.
  3. Büyükçangaz E, Şen A, Kahya S,2009: Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turk J Vet Anim Sci,33(4),311-316.
  4. Banai M, Mayer I, Cohen A, 1990: Isolation, identification, and characterization in Israel of brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. J Clin Microbiol, 28(5), 1057-1059.
  5. Corbel MJ, Gill KPW, Thomas EL, 1983: Methods for the identification of Brucella, Central Veterinary Laboratory, Booklet 2085, Weybridge Surrey KT15 3NB. Corbel MJ,1987:Brucella-phages: advance in the development of a reliable phage typing system for smooth and non-smooth Brucella isolates. Ann Inst Pasteur/Microbiol, 138,70. Corbel MJ,1989a:Brucellosis: epidemiology and prevalence worldwide. In "Brusellosis: Clinical and Laboratory Aspects", Ed; Young EJ, Corbel MJ, CRC Press,
  6. Inc., Boca Raton. Florida, USA, 25-40. Corbel MJ, 1989b: Microbiology of the genus Brucella. In " Brusellosis: Clinical and Laboratory Aspects", Ed;Young EJ, Corbel MJ, CRC Press,
  7. Inc., Boca Raton. Florida, USA, 54-67.
  8. Doğanay M, Aygen B,2003: Human brucellosis: an overview, Int J Infect Dis, 7,173-182.
  9. Erdenlig S, Sen A, 2000: Koyun atıklarından izole edilen Brucella cinsi mikroorganizmaların izolasyonu ve biyotiplendirilmesi. Pendik Vet Mikrobiol Derg, 31(2), 31-42.
  10. Erdenlig S, Iyisan AS, Baklan EA, Aksoy HY, 2007: Biovar distrubution of Brucella isolates from livestock in Turkey, 1999 to 2006. In: Proceedings of the15 th. International Congress of Mediterrenean Federation for Health and Production of Ruminants, 15-19 May 2007
  11. Pine Bay Resort Hotel, Kusadası, Turkey, p.27. Ewalt DR, Forbes LB, 1987: Atypical isolates of Brucella abortus from Canada and the United States characterized as dye sensitive with M antigen domi