SavedAdd your journal

Journals / Harran Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 3 - Sayı: 1

Identifying the Gender of a Black Swan (Cygnus atratus) by Using PCR

Bir Siyah Kuğuda (Cygnus atratus) PZR ile Cinsiyet Tespiti

Osman YAŞAR TEL
Faruk BOZKAYA
Bestami YILMAZ
Pages
30–32
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results indicated that the swan was female.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of the present study was to identify the gender of a black swan (Cygnus atratus) which was brought to Harran University Faculty of Veterinary Medicine by using polymerase chain reaction (PCR) technique.
📊
Key Findings & Data:Target region of CHD (chromodomain-helicase-DNA-binding) locus was amplfied with PCR by using 1237L-1272H or P2-P8 primer pairs and PCR products were separated by using agarose gel electrophoresis.
💡
Conclusion & Impact:The results indicated that the swan was female.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Siyah Kuğu · Cygnus atratus · cinsiyet

Abstract

The aim of the present study was to identify the gender of a black swan (Cygnus atratus) which was brought to Harran University Faculty of Veterinary Medicine by using polymerase chain reaction (PCR) technique. Target region of CHD (chromodomain-helicase-DNA-binding) locus was amplfied with PCR by using 1237L-1272H or P2-P8 primer pairs and PCR products were separated by using agarose gel electrophoresis. On agarose gel two bands were observed for both primer pairs. The results indicated that the swan was female.

Özet

Bu çalışmada, Harran Üniversitesi Veteriner Fakültesi’ne getirilen bir siyah kuğunun (Cygnus atratus) cinsiyetinin polimeraz zincir rekasiyonu (PZR) yöntemi ile belirlenmesi amaçlanmıştır. Bu amaçla CHD (chromodomain-helicase-DNAbinding) lokusunun hedef bölgesi iki farklı primer çifti (1237L-1272H ve P2-P8) yardımıyla PZR yöntemi ile çoğaltıldı ve PZR ürünleri agaroz jel elektorofrezi ile ayrımlandı. Her iki primer çifti ile elde edilen PZR ürünleri için agaroz jelde farklı uzunlukta iki bant gözlendi. Kuğunun dişi olduğu kanaatine varıldı.

🔖

Cite This Article

TEL, O. Y., BOZKAYA, F., YILMAZ, B. (2022). Identifying the Gender of a Black Swan (Cygnus atratus) by Using PCR. Harran Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 3 - Sayı: 1, 30–32. https://ajindex.com/articles/534394

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...