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Journals / Göztepe Tıp Dergisi / 2010 / Cilt: 25 - Sayı: 3

Down Sendromlu çocuklarda konjunktiva impresyon sitolojisi ve vitamin A düzeyleri ile ilişkisi

Conjunctival impression cytology and correlation with vitamin a levels in children with Down Syndrome

Yusuf NERGİZ
Sevin ÇAKMAK
Selen BAHÇECİ
Sevda SÖKER
Murat SÖKER
Pages
121–125
Publication Date
2022-10-17
DOI
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Results: A marked reduction of goblet cell dencity was found in the DS group (73.2±21.2 mm2) in comparison with the control group (113.5±30.1 mm2) (p

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Objective & Hypothesis:surface epithelium changes with conjunctival impression cytology (CIC), and to determine if we could detect ocular surface abnormalities in the eyes and to correlate with serum vitamin A levels in Down syndrome (DS) patients.
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Key Findings & Data:Material and Methods: The study groups included 36 eyes of 18 DS patients who were followed up at pediatrics clinics of our hospital and 40 eyes of 20 healthy control subjects from the same age group.
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Conclusion & Impact:Results: A marked reduction of goblet cell dencity was found in the DS group (73.2±21.2 mm2) in comparison with the control group (113.5±30.1 mm2) (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

surface epithelium changes with conjunctival impression cytology (CIC), and to determine if we could detect ocular surface abnormalities in the eyes and to correlate with serum vitamin A levels in Down syndrome (DS) patients. Material and Methods: The study groups included 36 eyes of 18 DS patients who were followed up at pediatrics clinics of our hospital and 40 eyes of 20 healthy control subjects from the same age group. Specimens for CIC were obtained with a cellulose acetate filter paper from the upper bulbar conjunctiva and fixed with 70 % ethyl alcohol, 37 % formaldehyde and 20:1:1 glicial asetic acid solution. Specimens were stained with periodic acid schiff’s and hematoxylin-eosin. Ocularsurface changes were evaluated on the cell content of the surface conjunctival epithelium by CIC with light microscopy. Serum vitamin A level was measured with HPLC in DS patients. Results: A marked reduction of goblet cell dencity was found in the DS group (73.2±21.2 mm2) in comparison with the control group (113.5±30.1 mm2) (p

Özet

Amaç: Down sendromlu(DS) hastalarda bulbar yüzey epitel değişikliklerini konjunktival impresyon sitolojisi tekniği ile saptamak ve serum vitamin A düzeyi ile korelasyonunu araştırmak Gereç ve Yöntem: Hasta grubunu, hastanemiz pediatri kliniğinde takip edilen 18 DS’lu hasta (36 göz), kontrol grubunu ise aynı yaş grubundaki 20 sağlıklı çocuk (40 göz) oluşturdu. Üst bulbar konjuktivaya uygulanan impresyon sitolojisi yöntemi ile sellüloz asetat kağıdı yüzeyine alınan örnekler (% 70’lik etil alkol, % 37’lik formaldehit ve glisial asetik asitin 20:1:1 oranındaki) solüsyonunda fikse edildi. Örnekler Periodik Asid Schiff ve Hematoksilen-eosin ile boyandı. Işık mikroskobisi ile oluşan okuler yüzey değişiklikleri değerlendirildi. DS hastalarda serum vitamin A düzeyi HPLC ile ölçüldü. Bulgular: DS'lu grupta goblet hücre yoğunluğu (73.2±21.2 mm2) kontrol grubuna göre (113.5±30.1 mm2) azalmış olarak saptandı (p

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Cite This Article

NERGİZ, Y., ÇAKMAK, S., BAHÇECİ, S., SÖKER, S., SÖKER, M. (2022). Down Sendromlu çocuklarda konjunktiva impresyon sitolojisi ve vitamin A düzeyleri ile ilişkisi. Göztepe Tıp Dergisi, Cilt: 25 - Sayı: 3, 121–125. https://ajindex.com/articles/526170

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