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Journals / Gazi Medical Journal / 2018 / Cilt: 29 - Sayı: 4

Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity

Sisplatin ile İndüklenmiş Uterus Toksisitesinde Asetil L-Karnitinin Koruyucu Etkisinin Araştırılması

Neslihan COŞKUN AKÇAY
Guleser GOKTAS
Gulistan Sanem SARIBAŞ
Seda Nur AKYOL
Seren Gulsen GURGEN
Candan OZOGUL
Deniz ERDOGAN
Ibrahim Murat HİRFANOĞLU
Sermin SEYHAN
Pages
294–298
Publication Date
2022-10-19
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Histological andimmunohistochemical investigations were performed, respectively.Results: We found that the number of TUNEL and caspases positive cells wereincreased in the endometrial epithelium, subepithelial connective tissue,endometrial glands and stroma in group III compare to the other groups.Furthermore inflammation and edema were observed in uterus of rats in groupIII.Conclusion: We can concluded that pretreatment of acetyl L-carnitineadministration has protective effect on histological alteration of uterus causedby cisplatin.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of the study was to investigate the prophylactic effects ofacetyl L-carnitine against to uterus induced by cisplatin.Methods: Twenty-four female Wistar albino rats were divided into four groups:group I (control) was administered with saline; group II was administered withacetyl L-carnitine; group III was administered with cisplatin; group IV waspretreated with acetyl L-carnitine before cisplatin intraperitoneal injection.
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Key Findings & Data:After72h of cisplatin injection uterine tissue was removed.
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Conclusion & Impact:Histological andimmunohistochemical investigations were performed, respectively.Results: We found that the number of TUNEL and caspases positive cells wereincreased in the endometrial epithelium, subepithelial connective tissue,endometrial glands and stroma in group III compare to the other groups.Furthermore inflammation and edema were observed in uterus of rats in groupIII.Conclusion: We can concluded that pretreatment of acetyl L-carnitineadministration has protective effect on histological alteration of uterus causedby cisplatin.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the study was to investigate the prophylactic effects ofacetyl L-carnitine against to uterus induced by cisplatin.Methods: Twenty-four female Wistar albino rats were divided into four groups:group I (control) was administered with saline; group II was administered withacetyl L-carnitine; group III was administered with cisplatin; group IV waspretreated with acetyl L-carnitine before cisplatin intraperitoneal injection. After72h of cisplatin injection uterine tissue was removed. Histological andimmunohistochemical investigations were performed, respectively.Results: We found that the number of TUNEL and caspases positive cells wereincreased in the endometrial epithelium, subepithelial connective tissue,endometrial glands and stroma in group III compare to the other groups.Furthermore inflammation and edema were observed in uterus of rats in groupIII.Conclusion: We can concluded that pretreatment of acetyl L-carnitineadministration has protective effect on histological alteration of uterus causedby cisplatin.

Özet

Amaç: Bu çalışmanın amacı, asetil L-karnitinin, sisplatinin neden olduğu uterus hasarına karşı profilaktik etkilerini araştırmaktır. Yöntem: Yirmi dört adet Wistar albino cinsi dişi sıçanlar dört gruba ayrıldı: 1. gruba (kontrol) salin uygulaması yapıldı; 2. gruba asetil L-karnitin uygulaması yapıldı; 3. gruba sisplatin uygulaması yapıldı; 4. grup ise sisplatinin intraperitoneal enjeksiyonu öncesinde asetil L-karnitin ile ön tedaviye tabi tutuldu. Sisplatin enjeksiyonundan 72 saat sonra uterus dokusu çıkarıldı. Histolojik ve immünohistokimyasal incelemeler yapıldı. Bulgular: TUNEL ve kaspaz pozitif hücre sayılarının, diğer gruplara göre 3. grupta endometriyal epitelde, subepitelyal bağ dokusunda, endometriyal bezlerde ve stromada arttığını bulduk. Ayrıca 3. Gruptaki ratların uterusunda inflamasyon ve ödem gözlenmiştir. Sonuç: Asetil L-karnitin ön tedavi uygulamasının, sisplatin kaynaklı uterus histolojisinin değişikliği üzerinde koruyucu etkisi olduğu sonucuna vardık.

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Cite This Article

AKÇAY, N. C., GOKTAS, G., SARIBAŞ, G. S., AKYOL, S. N., GURGEN, S. G., OZOGUL, C., ERDOGAN, D., HİRFANOĞLU, I. M., SEYHAN, S. (2022). Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. Gazi Medical Journal, Cilt: 29 - Sayı: 4, 294–298. https://ajindex.com/articles/519801

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References (14)

  1. Sonmezer M, Ozkavukcu S. Fertility preservation in females with malignant disease-1: causes, clinical needs and indications. Turk J Haematol 2009; 26:106-13.
  2. Familiari G, Caggiati A, Nottola SA, Ermini MD, Benedetto MR, Motta PM. Ultrastructure of human ovarian primordial follicles after combination chemotherapy for Hodgkin’s disease. Hum Reprod 1993; 8 (12): 2080-7.
  3. Fadillioglu E, Erdogan H, Sogut S, Kuku I. Protective effects of erdosteine against doxorubicin-induced cardiomyopathy in rats. J Appl Toxicol 2003; 23: 71-4.
  4. Fadillioglu E, Erdogan H. Effects of erdosteine treatment against doxorubicin-induced toxicity through erythrocyte and plasma oxidant/ antioxidant status in rats. Pharmacol Res 2003; 47: 317-22.
  5. DeVita VT, Hellman S, Rosenberg SA, editors. Cancer, principles & practice of oncology. 7th ed. Philadelphia: A Wolters Kluwer Co; 2005: 295-306. Kelland L. The resurgence of platinum-based cancer chemotherapy. Nat Rev Cancer 2007; 7: 573-84.
  6. Sonmezer M, Oktay K. Fertility preservation in female patients. Hum Reprod Update 2004; 10: 251-66.
  7. Warne GL, Fairley KF, Hobbs JB, Martin Fl. Cyclophoshamide-induced ovarian failure. N Engl J Med 1973; 289: 1159-62.
  8. Mackie EJ, Radford M, Shalet SM. Gonadal function following chemotherapy for childhood Hodgkin’s disease. Med Peidatr Oncol 1996; 27: 74-8.
  9. Delibas N, Ozcankaya R. Serbest Radikaller. SDÜ Tıp Fakültesi Dergisi 1995; 2: 11-17.
  10. Kalinec GM, Fernandez-Zapico ME, Urrutia R, Esteban-Cruciani N, Chen S, Kalinec F. Pivotal role of Harakiri in the induction and prevention of gentamicin-induced hearing loss. Proc Natl Acad Sci U S A 2005; 102: 16019-24. Gülçin I. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology. 2006; 217: 213-20. Al-Majed AA. Carnitine deficiency provokes cisplatin-induced hepatotoxicity in rats. Basic Clin Pharmacol Toxicol 2007; 100: 145-50.
  11. Pacifici EH, McLeod LL, Sevanian A. Lipid hydroperoxide-induced peroxidation and turnover of endothelial cell phospholipids. Free Radic Biol Med 1994; 17: 297-309.
  12. Madesh M, Balasubramanian KA. Activation of liver mitochondrial phospholipase A2 by superoxide. Arch Biochem Biophys 1997; 346: 187-92.
  13. Tufekci O, Gunes D, Ozogul C, Kolatan E, Altun Z, Yılmaz O, et al. Evaluation of the effect of acetyl L-carnitine on experimental cisplatin nephrotoxicity. Chemotherapy 2009; 55: 451-9.
  14. Coskun N, Hatipoglu MT, Ozogul C, Korkmaz C, Akyol SN, Micili SC, et al. The protective effects of acetyl L-carnitine on testis gonadotoxicit