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Journals / Experimental Biomedical Research / 2022 / Cilt: 5 - Sayı: 1

Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism

Recep AKKAYA
Sebahattin KARABULUT
Bilal ŞAHİN
Pages
48–57
Publication Date
2022-10-12
DOI
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Results: Administration of meloxicam before PTZ induced seizure, reduced seizure stages and prolonged FMJ duration (p

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Objective & Hypothesis:Aim: To investigate the possible anticonvulsive effect of the selective COX-2 inhibitor meloxicam in pentylenetetrazole (PTZ)-induced epileptic seizures in mice and to examine its possible role on inhibition and excitation balance in the brain.
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Key Findings & Data:Method: 30 BALB-c albino mice (16-18 weeks old) weighing 30-33 gr were used.
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Conclusion & Impact:Results: Administration of meloxicam before PTZ induced seizure, reduced seizure stages and prolonged FMJ duration (p
🔬Design:Research Article
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Abstract

Aim: To investigate the possible anticonvulsive effect of the selective COX-2 inhibitor meloxicam in pentylenetetrazole (PTZ)-induced epileptic seizures in mice and to examine its possible role on inhibition and excitation balance in the brain. Method: 30 BALB-c albino mice (16-18 weeks old) weighing 30-33 gr were used. Animals were randomly divided into five groups (n = 6 for each group). Group 1: control, group 2: received saline (10 ml/kg, i.p.) 30 minutes before PTZ (60 mg/kg i.p.) administration, group 3: received saline (10 ml/kg, i.p.) 30 minutes after PTZ (60 mg/kg i.p.) injection, group 4: received 60 mg/kg meloxicam i.p., 30 minutes before PTZ (60 mg/kg i.p.) administration. Group 5: received meloxicam (60 mg/kg i.p.) 30 minutes after PTZ injection (60 mg/kg, i.p.). The animals were observed for 30 minutes and the seizure stages and first myoclonic jerk times (FMJ) were recorded. After 24 hours, brain tissues were removed and the cortex and hippocampus were separated for biochemical assessments. ELISA method was used to measure GABA and glutamate levels. Results: Administration of meloxicam before PTZ induced seizure, reduced seizure stages and prolonged FMJ duration (p

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Cite This Article

AKKAYA, R., KARABULUT, S., ŞAHİN, B. (2022). Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazoleinduced acute seizures in mice through GABA and glutamate mediated mechanism. Experimental Biomedical Research, Cilt: 5 - Sayı: 1, 48–57. https://ajindex.com/articles/512584

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References (13)

  1. Taskiran AS, Ergul M, Gunes H, et al. The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole- Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line. Cell Mol. Neurobiol. 2021;41(1):173–83
  2. Rawat C, Kukal S, Dahiya UR, et al. Cyclooxygenase-2 (COX-2) inhibitors: future therapeutic strategies for epilepsy management. J Neuroinflammation. 2019;16(1):197
  3. Chen Z, Brodie MJ, Liew D, et al. Treatment outcomes in patients with newly diagnosed epilepsy treated with established and new antiepileptic drugs a 30-year longitudinal cohort study. JAMA Neurol. 2018;75(3):279–86
  4. Borowicz-Reutt KK, Czuczwar SJ. Role of oxidative stress in epileptogenesis and potential implications for therapy. Pharmacol Reports. 2020;72(5):1218–26
  5. Vezzani A, French J, Bartfai T, et al. The role of inflammation in epilepsy. Nat Rev Neurol. 2011;7(1):31–40
  6. Dhir A, Naidu PS, Kulkarni SK. Neuroprotective effect of nimesulide, a preferential COX-2 inhibitor, against pentylenetetrazol (PTZ)-induced chemical kindling and associated biochemical parameters in mice. Seizure. 2007;16(8):691–97
  7. Dhir A, Naidu PS, Kulkarni SK. Effect of cyclooxygenase-2 (COX-2) inhibitors in various animal models (bicuculline, picrotoxin, maximal electroshock-induced convulsions) of epilepsy with possible mechanism of action. Indian J Exp Biol. 2006;44(4):286–91
  8. Takemiya T, Suzuki K, Sugiura H, et al. Inducible brain COX-2 facilitates the recurrence of hippocampal seizures in mouse rapid kindling. Prostaglandins Other Lipid Mediat. 2003;71(3–4):205–16
  9. Zhang J, Goorha S, Raghow R, et al. The tissue-specific, compensatory expression of cyclooxygenase-1 and -2 in transgenic mice. Prostaglandins Other Lipid Mediat. 2002;67(2):121–35
  10. Kulkarni SK, Dhir A. Cyclooxygenase in epilepsy: From perception to application. Drugs of Today. 2009;45(2):135–54
  11. Löscher W, Klitgaard H, Twyman RE, et al. New avenues for anti-epileptic drug discovery and development. Nat Rev Drug Discov. 2013;12(10):757–76
  12. Edfawy M, Hassan MH, Mansour A, et al. Meloxicam modulates oxidative stress status, inhibits prostaglandin E2, and abrogates apoptosis in carbon tetrachlorideinduced rat hepatic injury. Int J Toxicol. 2012;31(3):276–86
  13. Darvishi H, Rezaei M, Khodayar MJ, et al. Differential effects of meloxicam on pentylenetetrazole- and maximal electroshock-induced convulsions in mice. Jundishapur J Nat Pharm Prod. 2017;12(2). e.