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Journals / Experimental Biomedical Research / 2022 / Cilt: 5 - Sayı: 3

99mTc-exendin-4: Radiolabeling and quality control studies of glucagon-like peptide analog

Makbule AŞIKOĞLU
Evren ATLIHAN GÜNDOĞDU
Derya İlem ÖZDEMİR
Emre ÖZGENÇ
Meliha EKİNCİ
Pages
235–243
Publication Date
2022-10-12
DOI
—
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Results: According to the results, 99mTc-exendin-4 was prepared with over 95 percent labeling efficiency by a novel, easy, and quick direct method with 30-min incubation time at pH 6.6.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Exendin-4 is a type 2 diabetes antidiabetic drug that is a peptide agonist of the glucagon-like peptide 1 receptor (GLP-1R).
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Key Findings & Data:Up to date, different exendin-4 compounds have been radiolabeled with many radioisotopes such as 68Ga, 18F, 64Cu and 99mTc for various purposes like study of over expression of GLP-1R in insulinoma.
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Conclusion & Impact:Results: According to the results, 99mTc-exendin-4 was prepared with over 95 percent labeling efficiency by a novel, easy, and quick direct method with 30-min incubation time at pH 6.6.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Exendin-4 is a type 2 diabetes antidiabetic drug that is a peptide agonist of the glucagon-like peptide 1 receptor (GLP-1R). Up to date, different exendin-4 compounds have been radiolabeled with many radioisotopes such as 68Ga, 18F, 64Cu and 99mTc for various purposes like study of over expression of GLP-1R in insulinoma. The purpose of this study is to radiolabel exendin-4 with 99mTc under appropriate conditions. Methods: In this study, exendin-4 was labeled with 99mTc, and quality control tests of 99mTc-exendin-4 were done using ascending radioactive thin layer chromatography (RTLC). Also, the effects of important parameters such as the amount of reducing agent, pH value, incubation time, and radiation dose on the labeling efficiency were investigated. Then, the stability of 99mTc-exendin-4 was assessed. Results: According to the results, 99mTc-exendin-4 was prepared with over 95 percent labeling efficiency by a novel, easy, and quick direct method with 30-min incubation time at pH 6.6. To achieve the best radiolabeling condition; 10 µg of exendin-4, 50 µg of stannous chloride (reducing agent) and 37 MBq 99mTc was used. The RTLC studies indicated that 99mTc-exendin-4 is stable up to 6 h in room temperature. Conclusion: The obtained results demonstrated that radiolabeled exendin-4 may be a promising agent for GLP-1R imaging studies. Further studies are in progress in order to evaluate receptor binding capacity and biodistribution of the complex in experimental animals.

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Cite This Article

AŞIKOĞLU, M., GÜNDOĞDU, E. A., ÖZDEMİR, D. İ., ÖZGENÇ, E., EKİNCİ, M. (2022). 99mTc-exendin-4: Radiolabeling and quality control studies of glucagon-like peptide analog. Experimental Biomedical Research, Cilt: 5 - Sayı: 3, 235–243. https://ajindex.com/articles/512580

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References (1)

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