SavedAdd your journal

Journals / Eurasian Journal of Veterinary Sciences / 2010 / Cilt: 26 - Sayı: 1

Boğa spermasının dondurulmasında soya lesitin temelli sulandırıcı ile birlikte kullanılan farklı yumurta sarısı konsantrasyonlarının sperma kalitesi üzerine etkileri

The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen

Purhan Barbaros TUNCER
Mustafa Numan BUCAK
Ali BİLGEN
Serpil SAROZKAN
Hüseyin KİNET
Serhat BÜYÜKLEBLEBİCİ
Pages
45–49
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: In Simmental bulls, semen extended with B5 had significantly higher CASA motility and CASA progressive motility than those extended with the rest of extenders after freezing and thawing (p0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Sariozkan S, Tuncer PB, Bucak MN, Buyukleblebici S, Kinet H, Bilgen A.
📊
Key Findings & Data:Eurasian J Vet Sci, 26, 1, 45-49 Aim: The centrifuged egg yolk (CEY) was added at concentrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell®) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane integrity assessed.
💡
Conclusion & Impact:Results: In Simmental bulls, semen extended with B5 had significantly higher CASA motility and CASA progressive motility than those extended with the rest of extenders after freezing and thawing (p0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Sariozkan S, Tuncer PB, Bucak MN, Buyukleblebici S, Kinet H, Bilgen A. The effects of different egg yolk concentrations used with soy lecithin-based extender on semen tuality to freeze bull semen. Eurasian J Vet Sci, 26, 1, 45-49 Aim: The centrifuged egg yolk (CEY) was added at concentrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell®) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane integrity assessed. Materials and Methods: Ejaculates obtained from each Simmental bull (n:12) divided in three equal aliquots and diluted in CEY 5% (B5), 10% (B10) supplemented, and without any CEY (B0) in soy bean lecithin - based extender, respectively. Thereafter, they were frozen and thawed following a standart protocol. Sperm cryosurvival was evaluated in vitro by microscopic assessments of post-thaw sperm motility (by means of the CASA), acrosomal and other abnormalities (head, mid-pieces, and tail) and plasma membrane integrity (evaluated by HOST). Results: In Simmental bulls, semen extended with B5 had significantly higher CASA motility and CASA progressive motility than those extended with the rest of extenders after freezing and thawing (p0.05). For VSL (p

Özet

Sarıözkan S, Tuncer PB, Bucak MN, Büyükleblebici S, Kinet H, Bilgen A. Boğa spermasının dondurulmasında soya lesitin temelli sulandırıcı ile birlikte kullanılan farklı yumurta sarısı konsantrasyonlarının sperma kalitesi üzerine etkileri. Eurasian J Vet Sci, 26, 1, 45-49 Amaç: Boğa spermasını dondurmak için soya lesitin temelli sulandırıcıya (Bioxcell®) %5 ve 10 konsantrasyonlarında santrifüj edilmiş yumurta sarısı (SYS) katıldı ve bunların çözüm sonu sperm motilite, morfolojik anormallikler ve membran bütünlüğü üzerindeki sinerjistik etkileri değerlendirildi. Gereç ve Yöntem: Her bir Simental boğadan alınan ejakulatlar (n=12) üç eşit miktara ayrıldı ve sırasıyla %5 (B5), %10 (B10) SYS eklenmiş ve hiç SYS (B0) içermeyen soya lesitin temelli sulandırıcı ile sulandırıldı. Ardından standart protokollere göre donduruldu ve çözdürüldü. Spermatozoa kryocanlılığı, in vitro çözüm sonu motilite (CASA), akrozomal ve diğer anormallikler ve plasma membran bütünlüğü (HOST) yönünden değerlendirildi. Bulgular: Simental boğalarda, dondurma ve çözdürme sonrası B5 ile sulandırılan grupta, diğer sulandırıcılarla sulandırılan gruplardan önemli derecede daha yüksek CASA motilite ve CASA progresif motilite oranı elde edilmiştir (p0.05). En yüksek VSL (p

🔖

Cite This Article

TUNCER, P. B., BUCAK, M. N., BİLGEN, A., SAROZKAN, S., KİNET, H., BÜYÜKLEBLEBİCİ, S. (2022). Boğa spermasının dondurulmasında soya lesitin temelli sulandırıcı ile birlikte kullanılan farklı yumurta sarısı konsantrasyonlarının sperma kalitesi üzerine etkileri. Eurasian Journal of Veterinary Sciences, Cilt: 26 - Sayı: 1, 45–49. https://ajindex.com/articles/510186

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Aboagla EM, Terada T, 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology, 62, 1160-1172.
  2. Aires VA, Hinsch KD, Mueller-Schloesser F, Bogner K, Mueller- Schloesser S, Hinsch E, 2003. In vitro and in vivo comparison of egg yolk-based and soybean lecithinbased extenders for cryopreservation of bovine semen. Theriogenology, 60, 269–279.
  3. Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennigs Z, Irvine DS, 1989. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod, 59, 1037–1046.
  4. Amirat L, Tainturier D, Jeanneau L, Thorin C, Gerard O, Courtens JL, Anton M, 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender. Theriogenology, 61, 895–907.
  5. Buckett WM, Luckas MJ, Aird IA, Farquharson RG, Kingsland CR, Lewis-Jones DI, 1997. The hypo-osmotic swelling test in recurrent miscarriage. Fertil Steril, 68, 506–509.
  6. Chatterjee S, De Lamirande E, Gagnon C, 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Mol Reprod Dev, 60, 498-506.
  7. Fernandez-Santos MR, Esteso MC, Montoro V, Soler AJ, Garde JJ, 2006. Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa: Effects of egg yolk, glycerol and cooling rate. Theriogenology, 66, 1931-1942.
  8. Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH, 2010. In vitro comparison of egg yolk-based and soy lecithin based extenders for cryopreservation of ram semen. Theriogenology, 73, 480-487.
  9. Fukui Y, Kohno H, Togari T, Hiwasa M, Okabe K, 2008. Fertility after artificial insemination using a soybeanbased semen extender (Andromed) in sheep. J Reprod Dev, 54, 286–289.
  10. Gil J, Rodriguez-Irazoqui M, Lundeheim N, Soderquist L, Rodriguez-Martinez H, 2003. Fertility of ram semen frozen in Bioxcell and used for cervical artificial insemination. Theriogenology, 59, 1157-1170.
  11. Holt WV, Abaigar T, Jabbour HN, 1996. Oestrous synchronization, semen preservation and artificial insemination in the Mohor gazelle (Gazella dama mhorr) for the establishment of a genome resource bank programme. Reprod Fertil Dev, 8, 1215–1222.
  12. Hu JH, Li QW, Zan LS, Jiang ZL, An JH, Wang LQ, Jia YH, 2010. The cryoprotective effect of low-density lipopro- teins in extenders on bull spermatozoa