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Journals / Eurasian Journal of Veterinary Sciences / 2012 / Cilt: 28 - Sayı: 1

Atların periferal kan mononükler hücrelerinde (PKMH) erken gebelik ve östrus siklusu sürecinde Toll-like reseptörlerinin (TLRs) ekspresyonu

Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle

Seyit Ali KAYIS
Mehmet Osman ATLI
Ahmet SEMACAN
Ercan KURAR
Aydin GUZELOGLU
Pages
15–20
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: According to these results, it may suggest that determination of TLRs gene expression in equine PBMCs during early pregnancy is not a good marker in understanding early equine pregnancy.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Practical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement.
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Key Findings & Data:Material and Methods: Blood samples were collected on days of ovulation (d0) and 8 during estrous and pregnancy from three same mares.
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Conclusion & Impact:Conclusion: According to these results, it may suggest that determination of TLRs gene expression in equine PBMCs during early pregnancy is not a good marker in understanding early equine pregnancy.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Practical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement. Immune tolerance against allogenic fetus is critically important during the early pregnancy. Tolllike Receptors (TLRs) are known to be an important part of innate immune system. Aim was to evaluate expression of TLRs in peripheral blood mononuclear cells (PBMCs) during the early pregnancy in mare. Material and Methods: Blood samples were collected on days of ovulation (d0) and 8 during estrous and pregnancy from three same mares. Total RNA samples were isolated from PBMCs and cDNA synthesis was performed. Expressions of TLRs at mRNA levels were quantified by using realtime RT-PCR in duplicates. Relative Expression Software Tool (REST2009) was used for statistical analysis. Results: TLRs (1 to 10) were found to be expressed in PMBCs by RT-PCR. However, real-time PCR measurements failed to show effects of pregnant or cyclic status on expression of TLRs. Conclusion: According to these results, it may suggest that determination of TLRs gene expression in equine PBMCs during early pregnancy is not a good marker in understanding early equine pregnancy.

Özet

Amaç: Günümüzde kısraklarda birçok gebelik teşhis metodu olmasına rağmen, mümkün olabilecek en erken zamanda gebeliğinin teşhis edilmesi için pratik, güvenilir ve ekonomik bir yönteme halen ihtiyaç bulunmaktadır. Allojenik fötusa karşı immun tolerans erken gebelik esnasında kritik bir öneme sahiptir. Toll-like reseptörlerinin (TLRs) doğal immun sistemin önemli bir parçası olduğu bilinmektedir. Sunulan çalışmada, kısraklarda erken gebelikte periferal kan mononükler hücrelerinde (PKMH) TLRs ekspresyonunun incelenmesi amaçlandı. Gereç ve Yöntem: Kan numuneleri aynı üç kısraktan östrus siklusunun ve gebeliğin 0 ve 8. günlerinde toplandı. Total RNA örnekleri PKMH’lerinden izole edildi ve cDNA sentezlendi. mRNA düzeyindeki TLRs ekspresyonları iki defa realtime PZR kullanılarak kuantifiye edildi. İstatistiksel analiz için Relative Expression Software Tool (REST2009) kullanıldı. Bulgular: RT-PZR ile PKMH’lerde TLRs (1-10) ekspresyonları tespit edildi. Bununla beraber, real-time PZR ölçümleri siklik veya gebelik durumunun TLRs ekspresyonu üzerine etkisini göstermekte başarısız kaldı. Öneri: Bu sonuçlara bakıldığında, atlarda gebeliğin erken döneminde PKMH’lerinde TLR genlerinin ekspresyonlarının gebeliğin erken anlaşılması için iyi bir markör olmadığı söylenebilir.

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Cite This Article

KAYIS, S. A., ATLI, M. O., SEMACAN, A., KURAR, E., GUZELOGLU, A. (2022). Atların periferal kan mononükler hücrelerinde (PKMH) erken gebelik ve östrus siklusu sürecinde Toll-like reseptörlerinin (TLRs) ekspresyonu. Eurasian Journal of Veterinary Sciences, Cilt: 28 - Sayı: 1, 15–20. https://ajindex.com/articles/510134

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