SavedAdd your journal

Journals / Eurasian Journal of Veterinary Sciences / 2012 / Cilt: 28 - Sayı: 4

Superoxide dismutase and antioxidant status of hens' granulosa cells exposed to lead and molybdenum

Kurşun ve molibdene maruz kalan tavuk granulusa hücrelerinin superoksid dismutaz ve antioksidan durumları

Marcela CAPCAROVA
Adriana KOLESAROVA
Alexander V. SIROTKIN
Pages
209–213
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

TAS of cells was decreased in experimental groups.Conclusion: Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: The aim of this study was to determine the activity of superoxide dismutase (SOD) and total antioxidant status (TAS) of ovarian granulosa cells of hens cultured in vitro after lead (Pb) and molybdenum (Mo) administrations.Material and Methods: Ovarian granulosa cells were incubated with lead acetate/ammonium molybdate administrations as follows: group A (0.09/0.09 mg/mL), group B (0.13/0.13 mg/mL), group C (0.17/0.17 mg/mL), group D (0.33/0.33 mg/mL), group E (0.5/0.5 mg/mL) and the control group without any additions for 18 h.Results: Antioxidant status of granulosa cells was dependent on Pb and Mo doses.
📊
Key Findings & Data:Higher doses of both metals caused significant reduction of SOD activity.
💡
Conclusion & Impact:TAS of cells was decreased in experimental groups.Conclusion: Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: The aim of this study was to determine the activity of superoxide dismutase (SOD) and total antioxidant status (TAS) of ovarian granulosa cells of hens cultured in vitro after lead (Pb) and molybdenum (Mo) administrations.Material and Methods: Ovarian granulosa cells were incubated with lead acetate/ammonium molybdate administrations as follows: group A (0.09/0.09 mg/mL), group B (0.13/0.13 mg/mL), group C (0.17/0.17 mg/mL), group D (0.33/0.33 mg/mL), group E (0.5/0.5 mg/mL) and the control group without any additions for 18 h.Results: Antioxidant status of granulosa cells was dependent on Pb and Mo doses. Higher doses of both metals caused significant reduction of SOD activity. TAS of cells was decreased in experimental groups.Conclusion: Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.

Özet

Amaç: Mevcut araştırmanın amacı in vitro kültüre edilen tavuk ovaryum granulosa hücrelerine kurşun (Pb) ve molibden (Mo) uygulaması sonrasında superoksid dismutaz (SOD) ve total antioksidan durum (TAS)'un belirlenmesidir.Gereç ve Yöntem: Ovaryum granulosa hücreleri farklı dozlarda kurşun asetat/amonyum molibdat uygulaması ile 18 saat inkübe edildi. Kurşun asetat/amonyum molibdat ise faklı dozlarda uygulandı; grup A (0.09/0.09 mg/mL), grup B (0.13/0.13 mg/mL), grup C (0.17/0.17 mg/mL), grup D (0.33/0.33 mg/mL), grup E (0.5/0.5 mg/mL) ve kontrol.Sonuçlar: Granulosa hücrelerinin antioksidan durumu Pb ve Mo dozuna bağlı olarak değişti. Her iki metalin yüksek dozu SOD aktivitesinde önemli düşmelere neden oldu. Hücrelerin TAS'u deneme gruplarında düşük belirlendi.Öneri: İz elementler hayvanların üreme sistemi üzerine doğrudan veya dolaylı olarak oksitatif strese neden olarak olumsuz etkilere neden olabilir.

🔖

Cite This Article

CAPCAROVA, M., KOLESAROVA, A., SIROTKIN, A. V. (2022). Superoxide dismutase and antioxidant status of hens' granulosa cells exposed to lead and molybdenum. Eurasian Journal of Veterinary Sciences, Cilt: 28 - Sayı: 4, 209–213. https://ajindex.com/articles/510101

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Agarwal A, Gupta S, Sharma RK, 2005. Role of oxidative stress in female reproduction. Reprod Biol Endocrin, 14, 3-28.
  2. Alhendawi RA, Kirkby EA, Pilbeam DJ, 2005. Evidence that sulphur deficiency enhances molybdenum transport in xylem sap of tomato plants. J Plant Nutr, 28, 1347-1353.
  3. Arpasova H, Capcarova M, Kalafova A, Lukac N, Kovacik J, Formicki G, Massanyi P, 2007. Nickel induced alteration of hen body weight, egg production and egg quality after an experimental pororal administration. J Environ Sci Health B, 42, 913-918.
  4. Bires J, Maracek I, Bartko P, Biresova M, Weissova T, 1995. Accumulationo f trace elements in sheep and the effects upon qualitative and quantitative ovarian changes. Vet Hum Toxicol, 37, 349-356.
  5. Capcarova M, Kolesarova A, Arpasova H, Massanyi P, Lukac N, Kovacik J, Kalafova A, Schneidgenova M, 2008. Blood biochemical dynamics and correlations in laying hens after experimental nickel administration. Int I Poult Sci, 7, 538-547.
  6. Capcarova M, Kolesarova A, Lukac N, Sirotkin A, Roychoud- hury S, 2009. Antioxidant status and selected biochemi- cal parameters of porcine ovarian granulosa cells ex- posed to lead in vitro. J Environ Sci Health Part A, 44, 1617-1623.
  7. Devine PJ, Perreault SD, Luderer U, 2012. Roles of reactive oxygen species and antioxidants in ovarian toxicity. Biol Reprod, 86, 1-10.
  8. Du Plessis SS, Van Niekert FE, Coetzer WA, 1999. The effect of dietary molybdenum and sulphate on the sexual ac- tivity and plasma progesterone concentrations of ewes. Small Rumin Res, 33, 71-76.
  9. Eidi A, Eidi M, Al-Ebrahim M, Rohani AH, Mortazavi P, 2011. Protective effects of sodium molybdate on carbon tet- rachloride-induced hepatotoxicity in rats. J Trace Elem Med Biol, 25, 67-71.
  10. Ho Y-S, Cargano M, Cao J, Bronson RT, Heimler I, Hutz RJ, 1998. Reduced fertility in female mice lacking copper- zinc superoxide dismutases. J Biol Chem, 273, 7765-7769. Jones DP, 2008. Radical-free biology of oxidative stress. Am J Physiol Cell Physiol, 295, C849-C868. Kan CA, 1994. Factors affecting absorption of harmful sub- stances from the digestive tract of poultry and their level in poultry products. World Poultry Sci J, 50, 39-53. Khan RU, 2011. Antioxidants and poultry semen quality. World Poultry Sci J, 67, 297-308.
  11. Kolesarova A, Capcarova M, Arpasova H, Kalafova A, Mas- sanyi P, Lukac N, Kovacik J, Schneidgenova M, 2008. Nickel-induced blood chemistry alterations in hens af- ter an experimental peroral administration. J Environ Sci Health B, 43, 625-632. K