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Journals / Eurasian Journal of Veterinary Sciences / 2015 / Cilt: 31 - Sayı: 1

Ultrasound homojenizasyonun karaciğer homojenatlarında süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ve lipid peroksit düzeylerine etkileri

Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates

Deniz S. ÖZDEMİR
Pınar P. AKALIN
Nuri BAŞPINAR
Pages
16–19
Publication Date
2022-10-18
DOI
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Activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein were measured in supernatant of homogenisated samples by spectrophotometric methods.Results: Superoxide dismutase, glutathione peroxidase, catalase activities and total protein levels in mechanical group were significantly different from sonication groups (P

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Objective & Hypothesis:Aim: In this study, effects of ultrasound homogenisation (Sonication) technique on the activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein in liver homogenates were investigated.Materials and Methods: Postmortem healthy fresh calf liver was used as the material.
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Key Findings & Data:Liver was sliced and grouped as mechanical homogenisation (2 min) and sonication group (2, 4, 6, 8 and 10 second sonication).
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Conclusion & Impact:Activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein were measured in supernatant of homogenisated samples by spectrophotometric methods.Results: Superoxide dismutase, glutathione peroxidase, catalase activities and total protein levels in mechanical group were significantly different from sonication groups (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: In this study, effects of ultrasound homogenisation (Sonication) technique on the activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein in liver homogenates were investigated.Materials and Methods: Postmortem healthy fresh calf liver was used as the material. Liver was sliced and grouped as mechanical homogenisation (2 min) and sonication group (2, 4, 6, 8 and 10 second sonication). Activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein were measured in supernatant of homogenisated samples by spectrophotometric methods.Results: Superoxide dismutase, glutathione peroxidase, catalase activities and total protein levels in mechanical group were significantly different from sonication groups (P

Özet

Amaç: Çalışmada mekanik ve ultrasound (sonikasyon) homojenizasyon tekniklerinin karaciğer süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ile lipid peroksitleri ve total protein düzeylerine etkileri araştırılmıştır.Gereç ve Yöntem: Bu amaçla taze dana karaciğeri küçük parçalara ayrılarak mekanik homojenizasyon (2 dk) ve sonikasyon (2, 4, 6, 8 ve 10 sn) grupları oluşturuldu. Süperoksit dismutaz, glutasyon peroksidaz, katalaz enzim aktiviteleri ile lipid peroksitleri ve total protein düzeyleri homojenatların süpernatantında spektrofotometrik yöntemlerle belirlendi.Bulgular: Süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ile total protein düzeylerinin, mekanik homojenizasyon gruplarında, sonikasyon grubuna göre önemli düzeyde farklı (P

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Cite This Article

ÖZDEMİR, D. S., AKALIN, P. P., BAŞPINAR, N. (2022). Ultrasound homojenizasyonun karaciğer homojenatlarında süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ve lipid peroksit düzeylerine etkileri. Eurasian Journal of Veterinary Sciences, Cilt: 31 - Sayı: 1, 16–19. https://ajindex.com/articles/510008

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