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Journals / Eurasian Journal of Veterinary Sciences / 2015 / Cilt: 31 - Sayı: 4

Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep.

Koyunlara Corynebacterium cutis lizatı uygulamasının serum sitokin düzeylerine etkisinin belirlenmesi

Orhan ÇORUM
Emre BAHÇİVAN
Ayşe ER
Burak DİK
Hatice ESER
Enver YAZAR
Pages
209–213
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Hemogram parameters did not change statistically significant, while fluctuations were determined (P

📌 3 Key Takeaways

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Objective & Hypothesis:Materials and Methods: In the research, 10 male yearling Merino sheep was used.
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Key Findings & Data:Recommended dose (8 mg, 0.4 mL) of Corynebacterium cutis lysate (Ultra-corn® Inj.) was administered subcutaneously at a single dose to each animal.
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Conclusion & Impact:Hemogram parameters did not change statistically significant, while fluctuations were determined (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Materials and Methods: In the research, 10 male yearling Merino sheep was used. Recommended dose (8 mg, 0.4 mL) of Corynebacterium cutis lysate (Ultra-corn® Inj.) was administered subcutaneously at a single dose to each animal. Before (0 hour, control) and after the treatments, blood samples were obtained from v. jugularis at 2, 4, 8, 12, 24, 48, 72 and 96 hours. Concentrations of serum Tumor necrosis factor alpha (TNFα), Interleukin (IL)-1β, IL-6 and IL-10 were determined with ELISA reader. Hemogram values (White blood cell, red blood cell, platelet, hematocrit, hemoglobin) were determined with hemocell counter, whereas biochemical values (Creatine kinase-MB, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total protein, albumin, blood urea nitrogen, creatinine) were analyzed with autoanalyzer. Results: Corynebacterium cutis lysate caused fluctuations on the cytokine levels (P>0.05). Hemogram parameters did not change statistically significant, while fluctuations were determined (P

Özet

Amaç: Mevcut araştırmanın amacı koyunlara Corynebacterium cutis lizatı uygulamasının serum sitokin düzeyleri üzerine etkisi ile birlikte hemogram ve rutin biyokimyasal parametrelere etkisini belirlemektir. Gereç ve Yöntem: Araştırmada 10 adet erkek Merinos toklu kullanıldı. Her bir hayvana önerilen dozda (8 mg, 0.4 mL) Corynebacterium cutis lizatı (Ultra-corn® Inj. Susp.) subkutan yolla bir kez uygulandı. Uygulamadan önce (0. saat, kontrol) ve sonraki 2, 4, 8, 12, 24, 48, 72 ve 96. saatlerde v. jugularisten kan örnekleri alındı. Elde edilen serumlardan tümör nekrozis faktör alfa (TNFα), interlöykin (IL)-1β, IL-6 ve IL-10 düzeyleri ELISA okuyucusunda belirlendi. Hemogram değerleri (Alyuvar, akyuvar, platelet, hematokrit, hemoglobin) kan hücresi sayım cihazında ve biyokimyasal (Kreatin kinaz-MB, alkalen fosfataz, alanin aminotransferaz, aspartat aminotransferaz, gamma glutamiltransferaz, total protein, albümin, kan üre nitrojen, kreatinin) değerler otoanalizörde ölçüldü. Bulgular: Corynebacterium cutis lizatı uygulamasının sitokin düzeylerinde dalgalanmalara neden olduğu gözlendi (P>0.05). Hemogram parametrelerinde istatistiki değişimlere neden olmazken, bazı biyokimyasal değerlerde (Alkalen fosfataz, total protein, albümin, kreatinin) dalgalanmalara neden olduğu tespit edildi (P

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Cite This Article

ÇORUM, O., BAHÇİVAN, E., ER, A., DİK, B., ESER, H., YAZAR, E. (2022). Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep.. Eurasian Journal of Veterinary Sciences, Cilt: 31 - Sayı: 4, 209–213. https://ajindex.com/articles/509990

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