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Journals / Eurasian Journal of Veterinary Sciences / 2019 / Cilt: 35 - Sayı: 3

The determination of effects of in ovo administrated bisphenol a on the development of thymus and proportion of alpha-naphthyl acetate esterase enzyme lymphocyte by using histological and enzymehistochemical methods in chicken

Yumurtaya verilen bisfenol a ’nın, tavuklarda timusun gelişimi ve perifer kan alfa naftil asetat esteraz pozitif lenfosit oranı üzerindeki etkilerinin histolojik ve enzim histokimyasal yöntemlerle belirlenmesi

Yasemin ÖZNURLU
Didem YILMAZ
Pages
144–151
Publication Date
2022-10-18
DOI
—
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The percentage of peripheral blood ANAE positive lymphocytes was significantly lower in the BPA-treated groups than in the control groups (p

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Objective & Hypothesis:Aim: The aim of this study is to the determination of effects of in ovo administrated Bisphenol A on the development of thymus and proportion of alpha-naphthyl acetate esterase enzyme lymphocyte by using histological and enzyme histochemical methods in chicken.Materials and Methods: For this purpose, 310 fertile eggs, weighed 50-50 g, of Isa Brown laying parent stock were divided into 5 groups as control, vehicle- control , 50 100, and 250 μg/egg BPA.
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Key Findings & Data:At the 13th, 18th and 21st days of incubation, 10 eggs were opened from each group and blood and thymus tissue samples were taken from the obtained embryos.
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Conclusion & Impact:The percentage of peripheral blood ANAE positive lymphocytes was significantly lower in the BPA-treated groups than in the control groups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: The aim of this study is to the determination of effects of in ovo administrated Bisphenol A on the development of thymus and proportion of alpha-naphthyl acetate esterase enzyme lymphocyte by using histological and enzyme histochemical methods in chicken.Materials and Methods: For this purpose, 310 fertile eggs, weighed 50-50 g, of Isa Brown laying parent stock were divided into 5 groups as control, vehicle- control , 50 100, and 250 μg/egg BPA. Test solutions were injected into yolk before incubation. At the 13th, 18th and 21st days of incubation, 10 eggs were opened from each group and blood and thymus tissue samples were taken from the obtained embryos. Results:On the 13th, 18th and 21st days of incubation, BPAtreated groups were found to be retarded embryonic development of thymus tissue compared to the control group. At the same time, in BPA-treated groups, lymphoid tissue had less cell density and the number of ANAE positive lymphocytes decreased. The percentage of peripheral blood ANAE positive lymphocytes was significantly lower in the BPA-treated groups than in the control groups (p

Özet

Amaç: Bu çalışmanın amacı yumurtaya verilen Bisfenol A’nın, tavuklarda timusun gelişimi ve perifer kan alfa naftil asetat esteraz (ANAE) pozitif lenfosit oranı üzerindeki etkilerininhistolojik ve enzimhistokimyasal metodlar kullanarak belirlemektir.Gereç ve Yöntem: Bu amaçla Isa Brown ırkı yumurtacı tavuklara ait 310 adet her biri 50-55 g ağırlığında olan döllü yumurta kontrol, taşıyıcı kontrol, 50, 100, 250 μg/yumurtaBPA olmak üzere 5 gruba ayrıldı. Test solüsyonları inkübasyondan önce yumurta sarısına enjekte edildi. Kuluçkanın 13, 18 ve 21. günlerinde her gruptan 10’ar adet yumurta açıldıve elde edilen embriyolardan kan ve timus dokusu örnekleri alındı.Bulgular: Kuluçkanın 13. 18. ve 21. günlerinde BPA uygulanan gruplarda kontrol grubuna göre timus dokusunun embriyonik gelişiminin geri olduğu tespit edildi. Aynı zamandaBPA verilen gruplarda lenfoid dokunun daha az hücre yoğunluğuna sahip olduğu ve ANAE pozitif lenfositlerin sayıca azaldığı dikkati çekti. Perifer kan ANAE pozitif lenfosit oranlarının da kontrol gruplarına göre BPA verilen gruplarda önemli oranda düştüğü tespit edildi (p

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Cite This Article

ÖZNURLU, Y., YILMAZ, D. (2022). The determination of effects of in ovo administrated bisphenol a on the development of thymus and proportion of alpha-naphthyl acetate esterase enzyme lymphocyte by using histological and enzymehistochemical methods in chicken. Eurasian Journal of Veterinary Sciences, Cilt: 35 - Sayı: 3, 144–151. https://ajindex.com/articles/509847

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References (8)

  1. Ablin RJ, Bruns GR, Guinan P, Bush IM, 1974. The effect of estrogen on the incorporation of H-thymidine by PHAstimulated human peripheral blood lymphocytes. J Immunol, 113,705–10. Ahmed SA, 2000. The immune system as a potential target for environmental estrogens (endocrine disrupters): a new emerging field. Toxicology, 150, 191-206.
  2. Ahmed WMS, Moselhy WA, Nabil TM, 2015. Bisphenol A toxicity in adult male rats: Hematological, biochemical and histopathological approach, Global Veterinaria, 14 (2), 228-238.
  3. Berg C, Blomqvist A, Holm L, Brandt et al., 2004. Embryonic exposure to oestrogen causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen. Reproduction, 128 (4), 455-61.
  4. Berg C, Halldin K, Brunström B, 2001. Effects of bisphenol A and tetrabromobisphenol A on sex organ development in quail and chicken embryos. Environmental Toxicology and Chemistry, 20 (12), 2836-40.
  5. Berg C, Halldin K, Fridolfsson AK, Brandt I, et al., 1999. The avian egg as a test system for endocrine disrupters: effects of diethylstilbestrol and ethynylestradiol on sex organ develop. Science of the Total Environment, 233, 1, 57-66.
  6. Chapin RE, Adams J, Boekelheide K, Jr et al., 2008. NTPCERHR Expert panel report on the reproductive and developmental toxicity of bisphenol a. Birth Defects Research (Part B), 83, 157–395. Clayton EMR,Todd M, Dowd JB, Aiello AE, 2011. “The impact of bisphenol A and triclosan on immune parameters in the U.S. population, NHANES 2003–2006”,Environmental Health Perspectives, 119(3), 390-396.
  7. Colborn T, Clement C, 1992. Chemically-induced alterations and fuctional development: The Wildlife / Human connection. Princeton Scientific Publishing Co., Inc., NJ. 403 pp. Crain DA, Eriksen M, Iguchi T, Jobling et al., 2007. An ecological assessment of bisphenol-A: evidence from comparative biology. Reproductive Toxicology, 24 (2), 225-39. Crossmon G, 1937. A modification of Mollory’ s connective tissue stain with a discussion of the principles involved. The Anotomical Record, 69 (1), 33-8. EFSA (European Food Safety Authority), 2017.
  8. Ursula Gundert-Remy, Johanna Bodin, Cristina Bosetti, Rex Fitz- Gerald, Annika Hanberg, Ulla Hass, Carlijn Hooijmans, Andrew A. Rooney, Christophe Rousselle, Henk van Loveren, Detlef Wölfle, Fulvio Barizzone, Cristina Croera, Claudio Putzu and Anna F. Castoldi, Bisphenol A (BPA) hazard assessment protocol, TECHNICAL REPORT. Fernandez MF, Arrebola JP, Taoufiki J, Naval´on et al., 2007. Bisphenol-A an