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Journals / Eurasian Journal of Veterinary Sciences / 2021 / Cilt: 37 - Sayı: 2

The expression of steroidogenic genes in ovine corpus luteum during early pregnancy

Nadir KOÇAK
Mehmet KÖSE
Mehmet Osman ATLI
Mustafa HİTİT
Bülent BÜLBÜL
Pages
93–100
Publication Date
2022-10-18
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Accordingly, it can be suggested that the steroidogenic pathway in the corpus luteum of ewe may transcriptionally regulate progesterone synthesis required for maintenance of early pregnancy

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: The goal of this study was to investigate the expression of steroidogenic genes in ovine corpus luteum during early pregnancy Materials and Methods: The animal model was designed as pregnancy; ewes were divided into three sub-groups, pregnancy 12th day: P12, pregnancy 16th day: P16, pregnancy 22th day: P22, and cyclic day 16 (C16).
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Key Findings & Data:The expression of steroidogenic genes (steroidogenic acute regulatory protein; StAR, cytochrome P450 side-chain cleavage; P450sscc, and 3b-hydroxysteroid dehydrogenase/delta5 delta4-isomerase; 3βHSD) was evaluated using qPCR, and mRNA localization of StAR was detected on P16 against C16 through in-situ hybridization.
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Conclusion & Impact:Accordingly, it can be suggested that the steroidogenic pathway in the corpus luteum of ewe may transcriptionally regulate progesterone synthesis required for maintenance of early pregnancy
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: The goal of this study was to investigate the expression of steroidogenic genes in ovine corpus luteum during early pregnancy Materials and Methods: The animal model was designed as pregnancy; ewes were divided into three sub-groups, pregnancy 12th day: P12, pregnancy 16th day: P16, pregnancy 22th day: P22, and cyclic day 16 (C16). The expression of steroidogenic genes (steroidogenic acute regulatory protein; StAR, cytochrome P450 side-chain cleavage; P450sscc, and 3b-hydroxysteroid dehydrogenase/delta5 delta4-isomerase; 3βHSD) was evaluated using qPCR, and mRNA localization of StAR was detected on P16 against C16 through in-situ hybridization. Results: The expression of StAR mRNA was higher on day P22 and P16 compared to P12 (p0,05). mRNA expression of P450scc was greater on day P22 and P16 than day P12 (p0,05). Also, mRNA expression of 3βHSD had a similar trend; it was higher on day P22 and P16 compared to P12 (p0,05). In in-situ hybridization, we did not detect StAR mRNA on cyclic day 16 (C16) while abundantly expressed in luteal cells in P16. Conclusion: The mRNA expression of steroidogenic genes may appear to play a critical role during early pregnancy in ewes. Accordingly, it can be suggested that the steroidogenic pathway in the corpus luteum of ewe may transcriptionally regulate progesterone synthesis required for maintenance of early pregnancy

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Cite This Article

KOÇAK, N., KÖSE, M., ATLI, M. O., HİTİT, M., BÜLBÜL, B. (2022). The expression of steroidogenic genes in ovine corpus luteum during early pregnancy. Eurasian Journal of Veterinary Sciences, Cilt: 37 - Sayı: 2, 93–100. https://ajindex.com/articles/509763

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