Determination of the effect of danofloxacin on 8-hydroxy-2-deoxyguanosine level

Results: In this study, 8-OHDG levels did not change (P>0,05) statistically, while temporal elevations in troponin I, CK-MB isoenzyme and AST levels were determined (P

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: The primary aim of this study is to determine the effect of danofloxacin administrations on 8-hydroxy-2-deoxyguanosine (8- OHDG) level, oxidative stress biomarker, in sheep.

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Key Findings & Data:Materials and Methods: In this study, 6 mg/kg/day (Subcutaneous) dose of danofloxacin was applied to 10 sheep for 14 days.

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Conclusion & Impact:Results: In this study, 8-OHDG levels did not change (P>0,05) statistically, while temporal elevations in troponin I, CK-MB isoenzyme and AST levels were determined (P

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Aim: The primary aim of this study is to determine the effect of danofloxacin administrations on 8-hydroxy-2-deoxyguanosine (8- OHDG) level, oxidative stress biomarker, in sheep. In addition, to determine on heart [Troponin I, creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST)], liver [Alkaline phosphatase (ALP), alanine aminotransferase (ALT)] and kidney [Blood urea nitrogen (BUN), creatinine] damage biomarkers and hemogram parameters [White blood cell count (WBC), red blood cell count (RBC), platelet count, hemoglobin, hematocrit]. Materials and Methods: In this study, 6 mg/kg/day (Subcutaneous) dose of danofloxacin was applied to 10 sheep for 14 days. Blood samples were taken on day 0 (control), 1, 3, 5, 7, 9, 11, 13 and 15 days. Serum 8-OHDG, troponin I and CK-MB isoenzyme levels were measured with ELISA reader, while serum LDH, AST, ALT, ALP, creatinine and BUN values were determined with autoanalyzer. Hemogram parameters were determined with hemocell counter. Results: In this study, 8-OHDG levels did not change (P>0,05) statistically, while temporal elevations in troponin I, CK-MB isoenzyme and AST levels were determined (P

Özet

Amaç: Bu araştırmanın birinci amacı koyunlara danofloksasin uygulamasının oksidatif stres biyobelirteci 8-hidroksi-2-deoksiguanozin (8-OHDG) üzerine etkisini belirlemektir. Ayrıca kalp [Troponin I, kreatin kinaz-MB (CK-MB) izoenzim, laktat dehidrogenaz (LDH), aspartat aminotransferaz (AST)], karaciğer [Alkalen fosfataz (ALP), alanin aminotransferaz (ALT)] ve böbrek [Kan üre nitrojen (BUN), kreatinin] hasar biyobelirteçleri ile hemogram [Akyuvar sayısı (WBC), alyuvar sayısı (RBC), platelet sayısı, hemoglobin, hematokrit] parametrelerine etkisini belirlemektir.Gereç ve Yöntem: Araştırmada 10 adet koyuna 6 mg/kg/gün (deri altı) dozunda danofloksasin 14 gün süresince uygulandı. 0. (Kontrol), 1., 3., 5., 7., 9., 11., 13. ve 15. günler kan örnekleri alındı. Serum 8-OHDG, troponin I ve CK-MB izoenzim düzeyi ELISA okuyucusunda, serum LDH, AST, ALT, ALP, kreatinin ve BUN değerleri otoanalizörde belirlendi. Hemogram parametreleri ise kan hücresi sayım cihazında ölçüldü.Bulgular: Araştırmada 8-OHDG düzeyinde istatistiki değişimler belirlenmezken (P>0,05), troponin I, CK-MB izoenzim ve AST değerinde geçici yükselmeler belirlendi (P

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Cite This Article

DİK, B., ER, A., YAZAR, E., KORKMAZ, Y., CANBAR, R., COŞKUN, D. (2022). Determination of the effect of danofloxacin on 8-hydroxy-2-deoxyguanosine level. Eurasian Journal of Veterinary Sciences, Cilt: 35 - Sayı: 4, 224–229. https://ajindex.com/articles/508171

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