Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2004 / Cilt: 1 - Sayı: 2

Spermatozoon separation and fertility of frozen-thawed rabbit semen

Dondurulmuş tavşan spermatozoon separasyonu ve dölverimi

Pages
97–103
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion; swim - down technique was found to be more effective in improving the quality of frozen thawed rabbit semen then the other two tecniques (GWF and GWFSD).

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to evaluate the effect of glass wool filtration (GWF), swim - down (SD) and combination of glass wool filtration and swim - down techniques (GWFSD) on frozen - thawed rabbit semen parameters and fertility.
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Key Findings & Data:Before intrauterine insemination (IUI) 3 of 4 aliquits were processed by GWF (Group I), SD (Group II) and GWFSD (Group III) techniques.
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Conclusion & Impact:In conclusion; swim - down technique was found to be more effective in improving the quality of frozen thawed rabbit semen then the other two tecniques (GWF and GWFSD).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to evaluate the effect of glass wool filtration (GWF), swim - down (SD) and combination of glass wool filtration and swim - down techniques (GWFSD) on frozen - thawed rabbit semen parameters and fertility. Frozen - thawed rabbit semen were divided into four equal aliquots. Before intrauterine insemination (IUI) 3 of 4 aliquits were processed by GWF (Group I), SD (Group II) and GWFSD (Group III) techniques. Fourth aliquits were used directly for IUI as a control group (Group IV). Semen samples were taken before and after sperm separation techniques and motility, live-dead spermatozoa ratio and concentration parameters were evaluated. While the highest mean percentage of motility and lowest live-dead spermatozoa ratios were detected after SD technique (P 0,05). In conclusion; swim - down technique was found to be more effective in improving the quality of frozen thawed rabbit semen then the other two tecniques (GWF and GWFSD). However there was no significant difference in the pregnacy rates between separation techniques. We have concluded that, further studies need be carried out on this subject.

Özet

Bu çalışmanın amacı; dondurulmuş çözdürülmüş tavşan spermasında glass wool filtrasyon (GWF), swim-down (SD) yöntemleri ile glass wool filtrasyon ve swim - down yöntemlerinin kombinasyonunun (GWFSD), spermatolojik parametreler ve dölverimi üzerine olan etkilerinin araştırılmasıdır. Dondurulmuş-çözdürülmüş tavşan spermaları 4 eşit hacme bölünmüştür. Intrauterin tohumlama (IUT) öncesi spermalardan üçüne sırasıyla GWF tGrup I), SD (Grup II) ve GWFSD (Grup III) uygulanmıştır. Dördüncü spermalar kontrol grubu olarak (Grup IV) direk IUT'da kullanılmıştır. Separasyon öncesi ve sonrası spermatolojik örnekler alınmış ve motilite, ölü/ canlı spermatozoa oranı ve yoğunluk parametreleri değerlendirilmiştir. En yüksek ortalama motilite ve en düşük ölü spermatozoa oranı SD yöntemi sonunda elde edilirken, en yüksek yoğunluklar GWF yöntemi sonunda elde edilmiştir (P < 0,05). Gebelik oranları kontrol grubunda, GWF, SD ve GWFSD sonunda-, sırasıyla; % 70, % 50, % 70 ve % 60 olarak belirlenmiş ve aralarında önemli bir fark bulunamamıştır. Çalışma sonunda dondurulmuş çözdürülmüş tavşan spermasına uygulanan separasyon yöntemleri arasında SD yönteminin spermatolojik parametreler açısından diğer iki yönteme (GWF ve GWFSD) göre daha etkin olduğu sonucuna varılmıştır. Bununla birlikte, gebelik oranları açısından separasyon yöntemleri arasında istatistiki bir farka rastlanmaması bu konu üzerine daha fazla araştırma yapılmasına ihtiyaç olduğunu göstermektedir.

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Cite This Article

DAŞKIN, A., DEMİRAL, Ö. (2022). Spermatozoon separation and fertility of frozen-thawed rabbit semen. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 1 - Sayı: 2, 97–103. https://ajindex.com/articles/504125

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