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Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 10 - Sayı: 3

Investigation of Bovine Leukosis Virus Infection in Blood and Milk Serum in Dairy Cattle by ELISA

Sütçü İneklerin Kan ve Süt Serumlarında Bovine Leukosis Virus Enfeksiyonunun ELISA ile Araştırılması

Oya BULUT
Oğuzhan AVCI
Orhan YAPICI
Irmak DİK
Kamil ATLI
Pages
171–176
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results showed that a total of 86 (20.09%) blood and 39 (9.11%) milk samples were detected positive for BLV antibodies.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to determine of antibodies against Bovine Leukosis Virus (BLV) in blood and milk serum and whether using milk serum instead of blood serum in serological tests for BLV could be preferred.
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Key Findings & Data:In this study, 428 unvaccinated 2 to 9 years old clinically healthy Holstein breed dairy cows were examined from some private businesses in the districts of Isparta (Keçiborlu, Gönen, Şarkikaraağaç, Aksu, Yalvaç, Sütçüler, Atağbey, Eğirdir) between March 2010 and September 2010.
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Conclusion & Impact:Results showed that a total of 86 (20.09%) blood and 39 (9.11%) milk samples were detected positive for BLV antibodies.
🔬Design:Research Article
📍Scope:2010 Verileri
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to determine of antibodies against Bovine Leukosis Virus (BLV) in blood and milk serum and whether using milk serum instead of blood serum in serological tests for BLV could be preferred. In this study, 428 unvaccinated 2 to 9 years old clinically healthy Holstein breed dairy cows were examined from some private businesses in the districts of Isparta (Keçiborlu, Gönen, Şarkikaraağaç, Aksu, Yalvaç, Sütçüler, Atağbey, Eğirdir) between March 2010 and September 2010. Both blood and milk serum samples were collected from the same animal for serological investigation and all samples were examined by Indirect Enzyme Linked Immunosorbent Assay (ELISA) for BLV antibodies. Results showed that a total of 86 (20.09%) blood and 39 (9.11%) milk samples were detected positive for BLV antibodies. According to the districts, the lowest prevalance was detected as 4% in Şarkikaraağaç and the highest prevalance was detected as 59.02% in Gönen by blood serum-ELISA. According to ELISA milk test results, the lowest prevalance was detected as 1.92% in Aksu and the highest prevalance was detected as 34.55% in Yalvaç. It was concluded that there was a fair similarity (К= 0.214) between serum-ELISA and milk-ELISA, therefore, using milk serum in serological tests for BLV can not be considered as an alternative to using blood serum

Özet

Bu çalışma kan ve süt serumlarında Bovine Leukosis Virus (BLV)'a karşı gelişen antikorların tespit edilmesi ve kan serumu yerine süt serumu örneklerinin kullanılıp kullanılamayacağının belirlenmesi amacıyla yapıldı. Mart-Eylül 2010 tarihleri arasında Isparta'nın ilçelerindeki (Keçiborlu, Gönen, Şarkikaraağaç, Aksu, Yalvaç, Sütçüler, Atağbey, Eğirdir) özel işletmelerde bulunan klinik olarak sağlıklı görünümde BLV'ye karşı aşılanmamış 428 baş Holştayn ırkı (yaşları 2 ile 9 arasında) sağmal inekten serolojik incelemeler amacıyla kan ve süt örnekleri alındı. Aynı hayvanlardan elde edilen kan ve süt örnekleri BLV'ye spesifik antikor varlığını tespit etmek amacıyla indirekt Enzyme Linked Immunosorbent Assay (ELISA) ile incelendi. Araştırma sonucu kan serumu örneklerinin 86 adedi (%20.09) ve süt serum örneklerinin 39 adedi (%9.11) BLV antikorları yönünden pozitif bulundu. İlçeler dikkate alındığında ELISA-kan serum testi ile en düşük seropozitiflik %4 olarak Şarkikaraağaç'da tespit edilirken, en yüksek seropozitiflik ise %59.02 ile Gönen'de tespit edildi. ELISA-süt testi bulguları değerlendirildiğinde en düşük seropozitiflik %1.92 ile Aksu'da, en yüksek seropozitiflik ise %34.55 ile Yalvaç'da tespit edildi. Sonuç olarak ELISA-kan ve ELISA-süt testleri arasında ortanın altında uyum tespit edildiği (?= 0.214) için BLV'nin serolojik teşhisinde kan serumu yerine süt serumu örneklerinin kullanılamayacağı sonucuna varıldı

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Cite This Article

BULUT, O., AVCI, O., YAPICI, O., DİK, I., ATLI, K. (2022). Investigation of Bovine Leukosis Virus Infection in Blood and Milk Serum in Dairy Cattle by ELISA. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 10 - Sayı: 3, 171–176. https://ajindex.com/articles/503931

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References (14)

  1. Alkan F, Oguzoglu TC, Timurkan MO, Karapinar Z. Characterisation of env and gag gene fragments of bovine leukemia viruses (BLVs) from cattle in Turkey. Arch Virol 2011; 156: 1891-6
  2. Angelos JA, Thurmond MC. Bovine Lymphoma. Smith BP. ed. In: Large Animal Internal Medicine, Fourth Edition. USA: Mosby Elsevier, 2009; pp. 1173-6
  3. Batmaz H, Çarlı KT, Şen A, Kennerman E, Minbay A, Yılmaz Z, Caner V, Baklacı C. Güney Marmara Bölgesi’nde enzootik bovine leukosis’in prevalansı ve bazı bakım-yetiştirme koşullarının incelenmesi. Tr J Vet Anim Sci 1999; 23: 261-8
  4. Baumgartener LE, Olson C. Host range of bovine leukosis: Preliminary report. Straub OC. ed. In: Current topics in veterinary medicine and animal science. Boston: Martinus Nijhoff Publishing, 1982; pp. 338-46
  5. Burny A, Bruck C, Chantrenne H, Cleuter Y, Dekegel D, Ghysdael J, Kettmann R, Leclercq M, Leunen J, Mammerickx M, Portetelle D. Bovine leukemia virus: Molecular biology and epidemiology. Klein G. ed. In: Viral Oncology. New York: Raven Press, 1980; pp. 231-89
  6. Camargos MF, Feliziani F, De Giuseppe A, Lessa LM, Reis JKP, Leite RC. Evaluation of diagnostic tests to bovine leukemia virus. RPCVAR 2007; 102: 169-73
  7. Chung YS, Prior HC, Duffy PF, Rogers RJ, Mackenzie AR. The effect of pasteurisation on bovine leukosis virus-infected milk. Australian Vet J 1986; 63(11): 379-80
  8. Evermann JF, DiGiacomo RF, Hopkins SG. Bovine leukosis virus: understanding viral transmission and the methods of control. Vet Med 1987; 82: 1051-8
  9. Garazi S, Hoida G, Trainin Z, Ungar-Waron H, Brenner J. De novo-infection of a large accredited BLV-free dairy herd. Israel Vet J 2001; 56(4): 12-8
  10. Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F, Balon H, Bouzar AB, Defoiche J, Burny A, Reichert M, Kettmann R, Willems L. Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel antiretroviral therapies in human. Retrovirology 2007; 16: 4-18
  11. Hafez SM, Sharif M, Al-Sukaryan A, DeLa-Cruz D. Preliminary studies on enzootic bovine leukosis in Saudi dairy farms. Dtsch Tierarztl Wschr 1990; 97(2): 61-3
  12. İyisan AS, Bitgel A, Özyörük F. İstanbul ilindeki süt sığırlarında enzootik bovine leukosis’in seroepidemiyolojisi. Pendik Vet Mikrobiyol Derg 1996; 2(27): 223-45
  13. Johnson R, Kaneene JB. Bovine leukemia virus and enzootic bovine leukosis. Vet Bull 1992; 62(4): 287-312
  14. Kale M, Özmen Ö, Şahinduran Ş, Yavru S. Üç sütçü inekte enzootik bovine lökozis (EBL) enfe