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Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 11 - Sayı: 1

Detection of diacyglycerol o-acyltransferase 1 (DGAT1) gene polymorphism by PCR-RFLP method in East Anatolion Red and Native Black cattle breeds at villages

Halk elinde yetiştirilen Holştayn, Doğu Anadolu Kırmızısı ve yerli kara sığır ırklarında diacylglycerol o-acyltransferase 1 (DGAT1) gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi

Bilal AKYÜZ
Onur BAL
Pages
7–13
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that HW equilibrium was not in DGAT1 locus in all three breeds examined.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The purpose of this work was to examine the allele structures of DGAT1 gene by RFLP method in 100 head of Holstein, 50 head East Anatolian Red and 50 head Anatolian Black cattle breeds.
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Key Findings & Data:In order to determine the DGAT1 alleles, the products obtained from PCR were digested with CfrI endonuclease enzyme.
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Conclusion & Impact:It was found that HW equilibrium was not in DGAT1 locus in all three breeds examined.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The purpose of this work was to examine the allele structures of DGAT1 gene by RFLP method in 100 head of Holstein, 50 head East Anatolian Red and 50 head Anatolian Black cattle breeds. In order to determine the DGAT1 alleles, the products obtained from PCR were digested with CfrI endonuclease enzyme. After digestion, a 411 bp single band in KK genotype; three bands (411, 208 and 203 bp) in KA genotype and two bands (208 and 203 bp) in AA genotype were observed. The AA genotypic frequency was found the highest in the Holstein breed; the KK genotypic frequency was found the highest in the Anatolian Black breed and the KA genotypic frequency was found the highest likewise in the Anatolian Black breed in DGAT1 gene. In this study, the A allele frequency was found higher than the K frequency allele in Holstein and East Anatolian Red cattle breeds. But, the K allele frequency was found higher than the A allele in only the Anatolian Black breed. It was found that HW equilibrium was not in DGAT1 locus in all three breeds examined.

Özet

Bu çalışmada, 100 baş Holştayn, 50 baş Doğu Anadolu Kırmızısı ve 50 baş Yerli Kara sığırında DGAT1 geninin allel yapısının RFLP yöntemi ile belirlenmesi amaçlanmıştır. DGAT1 allellerinin belirlenmesinde, elde edilen PCR ürünleri CfrI endonükleaz enzimi ile kesilmiştir. Kesim sonunda, KK genotipindeki bireylerde 411 bplik tek bant; KA genotipindeki bireylerde 411, 208 ve 203 bplik üç bant; AA genotipindeki bireylerde ise 208 ve 203 bplik iki bant gözlenmiştir. DGAT1 geni için incelenen ırklarda en yüksek AA genotip frekansı Holştayn ırkında, en yüksek KK genotip frekansı Yerli Kara ırkında ve en yüksek KA genotip frekansı yine Yerli Kara ırkında görülmüştür. İncelenen ırklardan Holştayn ve Doğu Anadolu Kırmızısı ırklarında A allelinin frekansı K alleline göre yüksek bulunmuştur. Ancak Yerli Kara ırkında K allelinin frekansı diğer ırklardan yüksek bulunmuştur. Çalışma sonunda incelenen ırkların DGAT1 lokusu yönünden HW dengesinden sapma görülmüştür.

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Cite This Article

AKYÜZ, B., BAL, O. (2022). Detection of diacyglycerol o-acyltransferase 1 (DGAT1) gene polymorphism by PCR-RFLP method in East Anatolion Red and Native Black cattle breeds at villages. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 11 - Sayı: 1, 7–13. https://ajindex.com/articles/503925

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References (13)

  1. Alipanah M, Kalashnikova L, Rodionov G. Association of prolactin gene variants with milk production traits in Russian Red Pied cattle. Iran J Biotech 2007; 5(3): 158-61
  2. Bennewitz J, Reinsch N, Paul S, Looft C, Weimann C, Erhardt G, Thaller G, Kühn C, Schwerin M, Thomsen H, Reinhardt F, Reents R, Kalm E. The DGAT1 K232A mutation is not solely responsible for the milk production quantitative trait locus on the bovine chromosome 14. J Dairy Sci 2004; 87: 431-42
  3. Bishop MD, Hawkins GA, Keener CL. Use of DNA markers in animal selection. Theriogenology 1995; 43: 61-70
  4. Bobe G, Beitz DC, Freeman AE, Lindberg GL. Effect of milk protein genotypes on milk protein composition and its genetic parameter estimates. J Dairy Sci 1999; 82(12): 2797-804
  5. Dybus A. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Black-and-White cattle. Arch Tierz 2002; 45(5): 421-8
  6. Dybus A, Grzesiak W, Szatkowska I, Błaszczyk P. Association between the growth hormone combined genotypes and dairy traits in Polish Black-and-White cows. Anim Sci Pap Rep 2004; 22(2): 185-94
  7. Fisher PJ, Spelman RJ. Verification of selective DNA pooling methodology through identification and estimation of the DGAT1 effect. Anim Genet 2004; 35: 201-5
  8. Grisart B, Coppieters W, Farnir F, Karim, L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P, Spelman R, Georges M, Snell R. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Res 2001; 12: 222-31
  9. Jeichitra V, Kandasamy N, Panneerselvam S. Milk protein polymorphism in Kangayam cattle. Trop Anim Health Prod 2003; 35(2): 147-53
  10. Joudrey EM, Lechniak D, Petrik J, King WA. Expression of growth hormone and its transcription factor, Pit-1, in early bovine development. Mol Reprod Dev 2003; 64: 275-83
  11. Kaupe B, Winter A, Fries R, Erhardt G. DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. J Dairy Res 2004; 71: 182-7
  12. Kepenek EŞ. Polymorphism of Prolactin (PRL), Diacylglycerol Acyltransferase1 (DGAT-1) and Bovine Solute Carrier Family 35 Member(SLC35A3) Genes in Native Cattle Breeds and Its Implication for Turkish Cattle Breeding. The Degree of Master. The Graduate School of Natural and Applied Sciences of Middle East Technical University. Ankara-Türkiye, 2007
  13. Kovács K, Völgyi-Csík J, Zsolnai A, Györkös I, Fésüs L. Associations between the AluI