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Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 17 - Sayı: 1

Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Region of Turkey

Sivas Yöresinde Sığır ve Kenelerde Theileria, Babesia ve Anaplasma Enfeksiyonlarının Araştırılması

Kursat ALTAY
Ahmet Duran ATAS
Erkan OZKAN
Yusuf Ziya OGRAK
Pages
32–38
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results of the blood and tick samples analysed by RLB and sequencing, T. buffeli (GenBank accesion number: KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084), one Babesia genotype (Babesia sp.

📌 3 Key Takeaways

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Objective & Hypothesis:This study was carried out to investigate the presence and distribution of Theileria, Babesia and Anaplasma species in cattle and their ticks from Sivas region.
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Key Findings & Data:A total of 314 EDTA-blood samples and 610 ticks were analysed.
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Conclusion & Impact:According to the results of the blood and tick samples analysed by RLB and sequencing, T. buffeli (GenBank accesion number: KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084), one Babesia genotype (Babesia sp.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Anaplasma · Babesia · kene

Abstract

This study was carried out to investigate the presence and distribution of Theileria, Babesia and Anaplasma species in cattle and their ticks from Sivas region. A total of 314 EDTA-blood samples and 610 ticks were analysed. A part of 18S and 16SrRNA genes of Theileria/Babesia and Anaplasma species were amplified from the genomic DNAs extracted of the blood samples and tick pools by with polymerase chain reaction (PCR). A total of 14 probes (two catchall, two genera and ten species-specific) were bound on a membrane and then the PCR products were tested by reverse line blot (RLB) assay. The partial sequences of the 18S and 16S rRNA genes of representative positive samples were determined. According to the results of the blood and tick samples analysed by RLB and sequencing, T. buffeli (GenBank accesion number: KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084), one Babesia genotype (Babesia sp. Sivas, KJ183081) and one Anaplasma genotype (Anaplasma sp. Sivas, KJ210855) were detected. Babesia sp. Sivas were found to be 99% identical with B. occultans, Babesia sp. Kashi and Babesia sp. Kayseri whereas Anaplasma sp. Sivas were found to be 99% and 98% identical with Anaplasma sp. Clone 7 and A. bovis, respectively. Overall prevalences of Theileria and Anaplasma infections in cattle were found to be 5.10% and11.15% by RLB, respectively. This study is the first molecular survey on species of Theileria, Babesia and Anaplasma in cattle and ticks from Sivas.

Özet

Bu çalışmada, Sivas yöresinde sığır ve kenelerde Theileria, Babesia ve Anaplasma türlerinin varlığı ve yaygınlığının araştırılması amaçlandı. Sığırlardan toplanan 314 EDTAlı kan ve 610 kene örneği analiz edildi. Kan ve kenelerden elde edilen genomic DNA’lardan polimeraz zincir reaksiyonu (PZR) ile Theileria, Babesia ve Anaplasma türlerinin 18S ve 16S rRNA parsiyal gen bölgeleri amplifiye edildi. Bir membran üzerine toplam 14 prob (iki cathall/genel, iki soy ve on tür) bağlandı ve PZR ürünleri reverse line blotting (RLB) ile analiz edildi. Pozitif örneklerin 18S ve 16S rRNA genlerinin kısmi dizileri belirlendi. RLB ve sekans analizi ile Sivas yöresinde sığır ve kenelerde; T. buffeli (KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084) ve bir Babesia genotipi (Babesia sp. Sivas, KJ183081) ile bir Anaplasma genotipi (Anaplasma sp. Sivas, KJ210855) tespit edildi. Babesia sp. Sivas izolatının, B. occultans, Babesia sp. Kashi ve Babesia sp. Kayseri izolatları ile %99, Anaplasma sp. Sivas izolatının ise Anaplasma sp. Clone 7 ile %99 ve A. bovis ile %98 benzerliğe sahip olduğu belirlendi. Sığırlarda Theileria ve Anaplasma enfeksiyonlarının genel prevalansı, RLB'de sırasıyla %5.10 ve %11.15 olarak bulundu. Bu çalışma Sivas yöresinde sığırlarda ve kenelerde Theileria, Babesia ve Anaplasma türlerinin belirlendiği ilk moleküler çalışma niteliğindedir.

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Cite This Article

ALTAY, K., ATAS, A. D., OZKAN, E., OGRAK, Y. Z. (2022). Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Region of Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 17 - Sayı: 1, 32–38. https://ajindex.com/articles/503693

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References (12)

  1. Aktas M, Altay K, Dumanli N. PCR-based detection of Theileria ovis in Rhipicephalus bursa adult ticks. Vet Parasitol 2006; 140(3-4): 259-63.
  2. Aktas M, Altay K, Dumanlı N. Molecular detection and identification of Anaplasma and Ehrlichia species in cattle from Turkey. Ticks Tick Borne Dis 2011; 2(1): 62-6.
  3. Aktas M, Ozubek S. Molecular and parasitological survey of bovine piroplasms in the Black Sea Region, including the first report of babesiosis associated with Babesia divergens in Turkey. J Med Entomol 2015; 52(6): 1344-50.
  4. Altay K, Aktas M, Dumanli N. Theileria annulata Tams1 geninin PCR-RFLP analizi. Turk Parazitol Derg 2007; 31(3): 173-5.
  5. Altay K, Aydin MF, Dumanli N, Aktas M. Molecular detection of Theileria and Babesia infections in cattle. Vet Parasitol 2008a; 158(4): 295-301.
  6. Altay K, Aydin MF, Uluisik U, Aktas M, Dumanli N. Theileria annulata ve Theileria buffeli’nin teşhisinde multiplex PCR’ın kullanılması. Turk Parazitol Derg 2008b; 32(1): 1-3.
  7. Altay K, Aktas M, Dumanli N. Detection of Babesia ovis by PCR in Rhipicephalus bursa collected from naturally infested sheep and goats. Res Vet Sci 2008c; 85(1): 116-9.
  8. Altay K, Dumanli N, Aktas M. A study on ovine tick-borne hemoprotozoan parasites (Theileria and Babesia) in the East Black Sea Region of Turkey. Parasitol Res 2012; 111(1): 149-53.
  9. Altay K, Dumanli N, Aktas M, Ozubek S. Survey of Anaplama infections in small ruminants from East part of Turkey. Kafkas Univ Vet Fak Derg 2014; 20(1): 1-4.
  10. Ciloglu A, Inci A, Yildirim A, Onder Z, Duzlu O. Babesia bigemina Kayseri/Türkiye suşunun in vivo ve in vitro izolatlarında apikal membran antijen-1 proteininin moleküler karakterizasyonu ve ekspresyonu. Ankara Univ Vet Fak Derg 2018; 65: 239-46. d'Oliveira C, van der Weide M, Jacquiet P, Jongejan F. Detection of Theileria annulata by PCR in ticks (Acari: İxodidae) collected from cattle in Mauritania. Exp Appl Acarol 1997; 21(5): 279-91.
  11. Donatien A, Lestoquard F. Rickettsia bovis, nouvelle espèce pathogène pour le bœuf. Bull Soc Pathol Exot 1936; 29: 1057-61.
  12. Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa F R. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int