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Journals / Erciyes Tıp Dergisi / 2005 / Cilt: 27 - Sayı: 2

Influence of adriamycin on nature and position of notochord

Adriamycin'in notokord yapısı ve yerleşimi üzerine etkisi

Harun ÜLGER
Çetin Hülya SORKUN
Saim ÖZDAMAR
Pages
70–74
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In the Ad treated embryos, the Nt was branched and split into two or three parts at various levels, and become thinner and had vacuoles.Conclusion: Abnormal localization and malformations of the Nt may lead to a significant congenital malformation in associated organs.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: The notochord (Nt) is believed to play an important role in the development of axial organs.
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Key Findings & Data:The aim of this study was to determine the nature and position of Nt malformation in rat embryos exposed to adriamycin (Ad).Materials and Methods: Time mated pregnant rats were injected daily with either Ad (2mg/kg, ip) or saline on days 6-9 of gestation.
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Conclusion & Impact:In the Ad treated embryos, the Nt was branched and split into two or three parts at various levels, and become thinner and had vacuoles.Conclusion: Abnormal localization and malformations of the Nt may lead to a significant congenital malformation in associated organs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Purpose: The notochord (Nt) is believed to play an important role in the development of axial organs. Abnormal development of the Nt has been considered to lead to a variety of congenital abnormalities. The aim of this study was to determine the nature and position of Nt malformation in rat embryos exposed to adriamycin (Ad).Materials and Methods: Time mated pregnant rats were injected daily with either Ad (2mg/kg, ip) or saline on days 6-9 of gestation. Their embryos were harvested on gestational day 13,5. Serial sections were analyzed histologically with routine Hematoxylene-eozin staining. Results: In normal embryos, the Nt was formed as a discrete axial structure in front of the neural tube and enveloped by perinotochordal mesenchyme. In the Ad treated embryos, the Nt was branched and split into two or three parts at various levels, and become thinner and had vacuoles.Conclusion: Abnormal localization and malformations of the Nt may lead to a significant congenital malformation in associated organs.

Özet

Amaç: Notokordun (Nt) aksiyal organların gelişiminde önemli bir rol oynadığı bilinmektedir. Nt’un anormal gelişiminin değişik kongenital anomalilere neden olacağı göz önünde tutulmaktadır. Bu çalışmanın amacı adriamisine (Ad) maruz kalmış sıçan embriyolarında Nt malformasyonlarının yapısı ve yerleşimini belirlemektir.Gereç ve Yöntem: Gebelik zamanı bilinen sıçanlara gebeliğin 6-9. günlerinde Ad (2 mg/kg, ip) veya serum fizyolojik enjekte edildi. Bunların embriyoları gebeliğin 13,5. gününde sezaryenle çıkarıldı. Seri kesitler Hematoxylen-eozin ile boyanarak histolojik olarak değerlendirildi.Sonuçlar: Normal embriyolarda, Nt nöral tüpün önünde ayrı bir aksiyel yapı olarak şekillenmişti ve perinotokordal mezenşim ile çevrelenmişti. Ad uygulanmış embriyolarda, Nt dallı bir yapı göstermekteydi, değişik seviyelerde iki veya üç parçaya bölünmüş, kalınlaşmış ve vakuolleşmişti. Tartışma: Nt’un bu anormal yerleşimi ve malformasyonları diğer ilişkili olduğu organlarda önemli kongenital malformasyonlara yol açabilir.

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Cite This Article

ÜLGER, H., SORKUN, Ç. H., ÖZDAMAR, S. (2022). Influence of adriamycin on nature and position of notochord. Erciyes Tıp Dergisi, Cilt: 27 - Sayı: 2, 70–74. https://ajindex.com/articles/502803

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