Journals / Ekoloji / 2010 / Cilt: 19 - Sayı: 77

Endemik Centaurea wiedemanniana’da genetik varyasyonun RAPD belirteçleri kullanılarak belirlenmesi

A study of genetic variation in endemic plant centaurea wiedemanniana by using RAPD markers

Journal
Ekoloji
Pages
1–8
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this vulnerable endemic species.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Centaurea wiedemanniana is an endemic plant species native to Turkey.
📊
Key Findings & Data:Genetic variability within and among 6 known natural populations of this plant was assessed by using RAPD markers.
💡
Conclusion & Impact:The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this vulnerable endemic species.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Centaurea wiedemanniana is an endemic plant species native to Turkey. The plant is known to have a narrow distribution in two abutting counties, Bilecik and Eskiþehir. Genetic variability within and among 6 known natural populations of this plant was assessed by using RAPD markers. High levels of genetic diversity were detected both at population (PPB= 62.83%, I= 0.301, $H _S$= 0.196) and species level (PPB= 94.65%, I= 0.429, $H _T$= 0.278). Analyses of molecular variance showed that among and within-population genetic variation accounted for 71.70% and 28.30% of the total genetic variation, respectively. A high degree of genetic differentiation was also found among populations ($G _{ST}$= 0.223). The genetic structure of C. wiedemanniana has probably been shaped by its breeding characteristics, habitat preference and short distance seed and pollen dispersal. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this vulnerable endemic species.

Özet

Centaurea wiedemanniana Türkiye'de yetişen endemik bir bitki türüdür. Bitkinin komþu iki il olan Bilecik ve Eskişehir'de dar bir yayılışa sahip olduğu bilinmektedir. Bu bitkinin bilinen 6 doğal populasyonunda populasyon içi ve populasyonlar arası genetik çeşitlilik RAPD belirteçleri kullanılarak belirlenmiştir. Hem populasyon (PPB= 62,83%, I= 0,301, $H _S$= 0,197) hem de tür (PPB= 94,65%, I= 0,429, $H _T$= 0,278) seviyesinde yüksek genetik çeþitlilik değerleri tespit edilmiştir. Moleküler varyans analizleri populasyonlar arası ve populasyon içi genetik çeşitlilik değerlerinin toplam genetik çeşitliliğin sırasıyla %71,70 ve %28,30'una karşılık geldiğini göstermiştir. Ayrıca, populasyonlar arasında yüksek derecede genetik farklılaşma bulunmuştur ($G _{ST}$= 0,223). C. wiedemanniana'nın genetik yapısını büyük olasılıkla üreme özellikleri, habitat tercihi ve kısa mesafeli tohum ve polen dağılımı şekillendirmektedir. Gözlenen genetik varyasyon değerleri bu hassas endemik tür için planlanan herhangi in situ ya da ex situ koruma programlarında mümkün olduğunca çok sayıda populasyonun dikkate alınması gerektiğini önermektedir.

🔖

Cite This Article

SOZEN, E., OZAYDIN, B. (2022). Endemik Centaurea wiedemanniana’da genetik varyasyonun RAPD belirteçleri kullanılarak belirlenmesi. Ekoloji, Cilt: 19 - Sayı: 77, 1–8. https://ajindex.com/articles/499131

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Andersen MC (1993) Diaspore morphology and seed dispersal in several wind-dispersed Asteraceae. American Journal of Botany 80, 487-492.
  2. Atienzar FA, Jha AN (2006) The random amplified polymorphic DNA (RAPD) assay and related tecniques applied to genotoxicity and carcinogenesis studies: A critical review. Mutation Research 613, 76- 102. Bancheva S, Geraci A, Raimondo FM (2006) Genetic diversity in the Centaurea cineraria group (Compositae) in Sicily using isozymes. Plant Biosystems 140, 10-16.
  3. Barrett SCH, Kohn JR (1991) Genetics and evolutionary consequences of small population size in plants: implications for conservation. In: Falk, DA, Holsinger KE (eds), Genetics and Conservation of Rare Plants, Oxford University Press, New York, 3-30.
  4. Bjerregaard L, Wolf PG (2008) Strong genetic differentiation among neighboring populations of a locally endemic Primrose. Western North American Naturalist 68, 66-75.
  5. Colas B, Olivieri I, Riba M (1997) Centaurea corymbosa, a cliff-dwelling species tottering on the brink of extinction: A demographic and genetic study. Proceedings of the National Academy of Sciences of the United States of America 94, 3471-3476.
  6. Crema S, Cristofolini G, Rossi M, Conte L (2009) High genetic diversity detected in the endemic Primula apennina Widmer (Primulaceae) using ISSR fingerprinting. Plant Systematics and Evolution 280, 29-36.
  7. Cruse-Sanders JM, Hamrick JL (2004) Genetic diversity in harvested and protected populations of wild American ginseng, Panax quinquefolius L. (Araliaceae). American Journal of Botany 91, 540-548.
  8. Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N (2000) Türkiye Bitkileri Kırmızı Kitabı (Eğrelti ve Tohumlu Bitkiler). Türkiye Tabiatını Koruma Derneği ve Van Yüzüncü Yıl Üniversitesi,
  9. Baışcan Ofset, Ankara. (In Turkish) Ellstrand NC (1992) Gene flow among seed plant populations. New Forests 6, 241-256.
  10. Excoffier L, Smouse P, Quattro J (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes; application to human mitochondrial DNA restriction data. Genetics 131, 479- 491. Freville H, Justy F, Olivieri I (2001) Comparative allozyme and microsatellite population structure in a narrow endemic plant species, Centaurea corymbosa Pourret (Asteraceae). Molecular Ecology 10, 879-889.
  11. George S, Sharma J, Yadon VL (2009) Genetic diversity of the endangered and narrow endemic Piperia yadonii (Orchidaceae) assessed with ISSR polymorphisms. American Journal of Botany 96, 2022-2030.
  12. Hamrick JL, G