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Journals / Ekoloji / 2010 / Cilt: 19 - Sayı: 75

The Determination of the genotoxic effects of genistein that an environmental pollutant on Drosophila melanogaster

Çevresel bir kirletici olan genisteinin Drosophila melanogaster üzerine genotoksik etkilerinin belirlenmesi

Handan UYSAL
Arif AYAR
Deniz ALTUN
Hakan AŞKIN
Journal
Ekoloji
Pages
82–87
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, the results of this study indicate that genistein was highly genotoxic in the SMART assay

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Genistein is a phytoestrogen that aquatic environment contaminant and is found in a wide variety of plantderived foods especially in soybeans and soy-based foods.
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Key Findings & Data:Third-instar larvae trans-heterozygous for two genetic markers mwh and flr, were treated by different concentrations (1, 3, 5 ve 10μM) of genistein.
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Conclusion & Impact:Consequently, the results of this study indicate that genistein was highly genotoxic in the SMART assay
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Genistein is a phytoestrogen that aquatic environment contaminant and is found in a wide variety of plantderived foods especially in soybeans and soy-based foods. In this study, the potential genotoxic effects of genistein which available from several factories waste were researched via using Drosophila wing somatic mutation and recombination test (SMART). Third-instar larvae trans-heterozygous for two genetic markers mwh and flr, were treated by different concentrations (1, 3, 5 ve 10μM) of genistein. According to our results, depending on the concentration increased, especially in small single type, the total mwh and the total clones positive results were observed. Consequently, the results of this study indicate that genistein was highly genotoxic in the SMART assay

Özet

Genistein, akuatik çevre kirleticisi olan bir bitkisel östrojendir ve özellikle soya ve soya temelli gıdalarda bol miktarda bulunmaktadır. Bu çalışmada, çeþitli fabrikaların atığında da bulunan genisteinin potansiyel genotoksik etkileri Drosophila kanat somatik mutasyon ve rekombinasyon testi (SMART) ile araþtırılmıþtır. mwh ve flr işaret genlerini taşıyan üç günlük trans-heterozigot larvalara farklı konsantrasyonlarda (1, 3, 5 ve 10μM) genistein uygulanmıþtır. Elde ettiğimiz sonuçlara göre, konsantrasyon artışına bağlı olarak, özellikle küçük tek tip, toplam mwh ve toplam klonlarda pozitif sonuçlar gözlenmiþtir. Sonuç olarak bu çalışmanın sonuçları, genisteinin SMART testinde yüksek derecede genotoksik etkili olduğunu göstermiştir.

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Cite This Article

UYSAL, H., AYAR, A., ALTUN, D., AŞKIN, H. (2022). The Determination of the genotoxic effects of genistein that an environmental pollutant on Drosophila melanogaster. Ekoloji, Cilt: 19 - Sayı: 75, 82–87. https://ajindex.com/articles/499114

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References (13)

  1. Adlercreutz H (1995) Phytoestrogens: epidemiology and a possible role in cancer protection. Environmental Health Perspectives 103, 103-112.
  2. Akiyama T, Ishida J, Nakagava S, Ogawara S, Watanabe S, Itoh N, Shiyba M, Fukami Y (1987) Genistein, a specific inhibitor of tyrosine-specific protein kinases. Journal of Biological Chemistry 262, 5592-5595.
  3. Amaral VS, Sinigaglia M, Reguly ML, Andrade HHR (2006) Genetic Toxicity in surface water from Guaiba Hydrographic Region under the influence of industrial, urban and agricultural sewage in the Drosphila Wing-Spot Test. Environmental Pollution 139, 469-476.
  4. Anderson D, Dobrzynska M, Basaran N (1997) Effect of various genotoxins and reproductive toxins in human lymphocytes and sperm in the comet assay. Teratogenesis, Carcinogenesis, and Mutagenesis 17, 29-43. Aşkın H, incekara Ü (2010) The determination of the genotoxic effect of Karasu River surface water containing local fat plant wastewater by Drosophila wing somatic mutation and recombination test (SMART). Ekoloji 19, 74, 45-50.
  5. Beck V, Rohr U, Jungbauer A (2005) Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy? Journal of Steroid Biochemistry and Molecular Biology 94, 499-518.
  6. Boos G, Stopper H (2000) Genotoxicity of several clinically used topoisomerase II inhibitors. Toxicology Letters 116, 7-16.
  7. Constantinou A, Kiguchi K, Huberman E (1990) Induction of differentiation and DNA strand breakage in human HL-60 and K-562 leukemia cells by genistein. Cancer Research 50, 2618-2624.
  8. Constantinou A, Mehta R, Runyan C, Rao K, Vaughan A, Moon R (1995) Flavonoids as DNA topoisomerase antagonists and poisons: structure-activity relationships. Journal of Natural Products 58, 217-225.
  9. Di Virgilio AL, Iwami K, Watjen W, Kahl R, Degen GH (2004) Genotoxicity of the isoflavones genistein, daidzein, and equol in V79 cells. Toxicology Letters 151, 151-162.
  10. Dixon RA (2004) Phytoestrogens. Annual Review of Plant Biology 55, 225-261.
  11. Ferguson LR, Baguley BC (1996) Mutagenicity of anticancer drugs that inhibit topoisomerase enzymes. Mutation Research 355, 91-101.
  12. Frei H, Würgler FE (1988) Statistical methods to decide whether mutagenic test data from Drosophila assays indicate a positive, negative or inconclusive results. Mutation Research 203, 297-308.
  13. Frei H, Würgler FE (1996) Induction of somatic mutation and recombination by four inhibitors of eukaryotic topoisomerases assayed in the wing spot test of Drosophila melanogaster. Mutagenesis 11,