Journals / Ekoloji / 2014 / Cilt: 23 - Sayı: 91

Kadmiyum Stresi Altındaki Trametes (Coriolus) versicolor ve Funalia trogii 'de Poliamin ve Lakkaz Üretimi

Polyamine and Laccase Production under Cadmium Stress in Trametes (Coriolus) versicolor and Funalia trogii

Journal
Ekoloji
Pages
29–35
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This increase under Cd stress suggests their possible role in combatingthe adverse effect of heavy metal stress.

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study the effect of increasing the concentrations of the heavy metal cadmium (Cd) (0.1-200ppm) on the free polyamines (PAs) level and laccase activity was studied in the white rot fungi Trametes(Coriolus) versicolor and Funalia trogii at 6 and 15 days.
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Key Findings & Data:The highest Spd levels(1952.05 ?g/DW and 2391.54 ?g/DW) were observed in 1 ppm Cd at 15 days of incubation in T. versicolorand F. trogii respectively.
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Conclusion & Impact:This increase under Cd stress suggests their possible role in combatingthe adverse effect of heavy metal stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In the present study the effect of increasing the concentrations of the heavy metal cadmium (Cd) (0.1-200ppm) on the free polyamines (PAs) level and laccase activity was studied in the white rot fungi Trametes(Coriolus) versicolor and Funalia trogii at 6 and 15 days. Spermidine (Spd) was the most abundant PA in the two white rot fungi, while Spermine (Spm), andPutrescine (Put) were found only in trace amounts or were not determined. The highest Spd levels(1952.05 ?g/DW and 2391.54 ?g/DW) were observed in 1 ppm Cd at 15 days of incubation in T. versicolorand F. trogii respectively. The laccase activity of both species, especially in the 15-day cultures, showed asignificant increase compared to the control. The highest increase was evident at 100 ppm Cd where thelaccase activity was over 19 fold higher than that of the control sample in the 15 day culture of T. versicolor.The dry biomass in the 0.1 and 1 ppm Cd concentrations showed a significant increase in T. versicolor in thesix and 15 day incubation periods. In all cases, the dry biomass in both the six and 15 day cultures of F. trogiishowed a significant increase compared to the control cultures.The findings gathered through this study show that Cd can amplify free Spd levels and increase laccaseactivity in T. versicolor and F. trogii. This increase under Cd stress suggests their possible role in combatingthe adverse effect of heavy metal stress.

Özet

Bu çalışmada, artan konsantrasyondaki kadmiyum (Cd) (0.1-200 mg/L) uygulamasının, ağır metalcevabında indikatör olan serbest poliaminlerin düzeyine ve lakkaz aktivitelerine olan etkisi beyaz çürükçülfunguslardan Trametes (Coriolus) versicolor and Funalia trogii ' de 6. ve 15. günlerde araştırıldı.Spermidin (Spd) her iki beyaz çürükçül fungusta da en yoğun bulunurken, Spermin (Spm) ve Putresin(Put) sadece eser düzeyde bulundu ya da belirlenemedi. En yüksek Spd düzeyi (1952.05 ?g/DW; 2391.54?g/DW) 1 ppm Cd uygulanan 15 günlük inkübasyon süresindeki sırasıyla T. versicolor and F. trogii ' de tespitedildi. Her iki türdeki lakkaz aktivitesi özellikle 15 günlük kültürlerde kendi kontrollarına göre önemlidüzeyde artış gösterdi. Lakkaz aktivitesinde en yüksek artış, 100 ppm Cd uygulanan 15 günlük T. versicolorkültürlerinde kontrol grubuna göre 19 kat daha yüksek bulunarak elde edildi. Kuru ağırlık 0.1 ve 1 ppm Cduygulanan 6 ve 15 günlük T. versicolor kültürlerinde belirgin bir artış göstermiştir. 6 ve 15 günlük F. trogiikültürlerinde bütün uygulama gruplarında elde edilen kuru ağırlık kontrol grubu ile kıyaslandığındabelirgin bir artışa neden olmuştur. Bu çalışma sonucu, kadmiyum uygulanan T. versicolor ve F. trogii kültürlerinde serbest Spd düzeyi ve lakkazaktivitesinde artış olduğunu gösterdi. Cd stresi altındaki T. versicolor ve F. trogii 'de serbest Spd düzeyi velakkaz aktivitesindeki artış ağır metal stresi ile mücadelede rol oynadığı fikrini verdi.

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Cite This Article

MUTLU, F., APOHAN, E., KAHRAMAN, S. (2022). Kadmiyum Stresi Altındaki Trametes (Coriolus) versicolor ve Funalia trogii 'de Poliamin ve Lakkaz Üretimi. Ekoloji, Cilt: 23 - Sayı: 91, 29–35. https://ajindex.com/articles/498965

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