Journals / Ekoloji / 2014 / Cilt: 23 - Sayı: 90

Farklı Buğday Çimi Çeşitlerinin Antioksidan Aktiviteleri ve Fenolik Bileşiklerin Tayini İçin YeniBir Analitik Metod

A New Analytical Method for the Determination ofPhenolic Compounds and Their AntioxidantActivities in Different Wheat Grass Varieties

Journal
Ekoloji
Pages
73–80
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When wheat grass will be used as a natural food preservative,carcinogenic effects of synthetic food preservatives for body would be preserved

📌 3 Key Takeaways

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Objective & Hypothesis:In this work, the phenolic content and antioxidant capacity of wheat grass, Turkish Amber Durum wheatwas studied.
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Key Findings & Data:For this purpose Turkish Amber Durum wheat was germinated at three different times (15, 30and 40 days).
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Conclusion & Impact:When wheat grass will be used as a natural food preservative,carcinogenic effects of synthetic food preservatives for body would be preserved
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this work, the phenolic content and antioxidant capacity of wheat grass, Turkish Amber Durum wheatwas studied. For this purpose Turkish Amber Durum wheat was germinated at three different times (15, 30and 40 days). All the wheat samples sprouted in the same organic conditions and then dried with air andwithout sunlight and placed into an incubator at 50°C overnight. The dried and milled amber wheat grasswas extracted with ethyl acetate and compared for their antioxidant capacities against the 2,2-diphenyl-1picrylhydrazyl (DPPH.) radical, and at the same time the total phenolic content (TPC) was determined.Phenolic compounds in the extracts were examined by high performance liquid chromatography (HPLC).In this study, by using a new method, nine standards were separately detected within 50 min by using theHPLC system, as previously reported by other authors. The major phenolics, identified in the wheat grass that consisted of ferulic acid, gallic acid, caffeic acid, pcoumaric acid, ellagic acid, benzoic acid, p-hydroxybenzoic acid, syringic acid, quercetin and bha (butylatedhydroxyanisole). The highest antioxidant and antiradical activities were detected in Turkish Amber wheatthat sprouted in 15 days. This study demonstrates that the importance of wheat grass is due to its highantioxidant activity. Consumption of wheat grass has positive effects on human health such as reduced riskof coronary heart diseases, certain type of cancers and stroke. Due to the high antioxidant effect of wheatgrass the aim is to use it as a food preservative. When wheat grass will be used as a natural food preservative,carcinogenic effects of synthetic food preservatives for body would be preserved

Özet

Bu çalışmada, Türk Amber Durum buğdayının fenolik içeriği ve antioksidan kapasitesi incelendi. Buamaçla, Türk Amber Durum buğdayı (15, 30 ve 40 gün süre ile) üç farklı zamanda çimlendirilmiştir.Filizlenen buğdaylar, aynı organik koşullarda güneş ışığı olmadan açık havada kurutuldu ve 50°C geceboyunca inkübatör de bırakıldı. Kurutulmuş ve öğütülmüş buğday çimi etil asetat ile ekstrakte edildi ve 2,2difenil-1-picrylhydrazyl (DPPH.), radikaline karşı antioksidan kapasiteleri kıyaslandı ve toplam fenolikiçeriği (TFİ) de tayin edildi. Ekstraktlardaki fenolik bileşikler, yüksek performanslı sıvı kromatografisi(YBSK) ile incelenmiştir. Bu çalışmada, yeni bir metod kullanılarak 10 farklı standart, yaklaşık 50 dakikalık YBSK sistemiyle, öncedendiğer yazarlar tarafından rapor edildiği gibi, ayrı ayrı analiz edilmiştir. Buğday çiminde belirlenen başlıcafenolik bileşikler ferulik asit, gallik asit, kafeik asit, p-kumarik asit, ellagik asit, benzoik asit, phidroksibenzoik asit, şiringik asit, kuersetin ve bha'dan oluşuyordu. En yüksek antioksidan ve antiradikalaktivite 15 gün içinde çimlenmiş esmer buğday çiminde tespit edilmiştir. Bu çalışma, buğday çimininyüksek antioksidan aktivitesine bağlı olarak önemini ortaya koymıştur. Buğday çimi tüketiminin insansağlığı üzerinde koroner kalp hastalıkları, belirli kanser türleri ve felç riskini azaltmak gibi olumlu etkilerivardır. Buğday çiminin yüksek antioksidan etkisi nedeniyle, gıda katkı maddesi olarak kullanımı hedeflenmektedir.Buğday çimi doğal bir gıda katkı maddesi olarak kullanıdığında, sentetik gıda koruyucu maddelerin vücutiçin kanserojen etkileri önlenmiş olacaktır

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Cite This Article

KARDAS, T. A., DURUCASU, I. (2022). Farklı Buğday Çimi Çeşitlerinin Antioksidan Aktiviteleri ve Fenolik Bileşiklerin Tayini İçin YeniBir Analitik Metod. Ekoloji, Cilt: 23 - Sayı: 90, 73–80. https://ajindex.com/articles/498953

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References (14)

  1. Adom KK, Liu RH (2002) Antioxidant activity of grains. Journal of Agriculture and Food Chemistry 50: 6182-6187.
  2. Bonoli M, Verardo V, Marconi E, Caboni MF (2004) Antioxidant phenols in barley (Hordeum vulgare L.) flour: comparative spectrophotometric study among extraction methods of free and bound phenolic compounds. Journal of Agricultural and Food Chemistry 52: 5195-5200
  3. Chew YL, Lim YY, Omar M, Khoo KS (2008) Antioxidant activity of three edible seaweeds from two areas in South East Asia, LWT - Food Science and Technology 41: 1067-1072.
  4. Craig SAS, Holden JF, Troup JP, Auerbach MH, Frier HI (1998) Polydextrose as soluble fiber: Physiological and analytical aspects. Cereal Food World 43: 370-376.
  5. Graf E (1992) Antioxidant potential of ferulic acid. Free Radical Biological Medicine 13: 435-448
  6. Hatcher DW, Kruger JE (1997) Simple phenolic acids in flours prepared from Canadian wheat relationship to ash content, color and polyphenol oxidase activity. Cereal Chemistry 74: 337-343
  7. Heim KE, Tagliaferro AR, Bobilya DJ (2002) Flavonoid antioxidants: chemistry, metabolism and structure- activity relationships. The Journal of Nutritional Biochemistry 13: 572-584.
  8. Herrera MC, Luque MD de Castro (2005) Ultrasound-assisted extraction of phenolic compounds from strawberries prior liquid chromatographic separation and photodiode array ultraviolet detection. Journal of Chromatography 1100: 1-7.
  9. Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. Journal of Agriculture and Food Chemistry 53: 1841-1856.
  10. Hung PV, Morita N (2008) Distribution of phenolic compounds in the graded flours milled from whole buckwheat grains and their antioxidant capacities. Food Chemistry 109: 325-331.
  11. Hung PV, Maeda T, Morita N (2009) Total phenolic compounds and antioxidant capacity of wheat graded flours by polishing method. Food Research International 42: 185-190.
  12. Jimenez-Escrig A, Jimenez-Jimenez I, Sanchez-Moreno C, Saura-Calixto F (2000) Evaluation of free radical scavenging of dietary carotenoids by the stable radical 2, 2-diphenyl-1-picrylhydrazyl. Journal of the Science of Food and Agriculture, 80: 1686-1690.
  13. Liyana-Pathirana CM, Shahidi F (2006) Importance of insoluble-bound phenolics to antioxidant properties of wheat. Journal of Agriculture and Food Chemistry 54: 1256-1264.
  14. Mattila JM, Hellstrom P, Hellstrom J (2005) Contents of phenolic acids, alkyl- and alkenylresorcinols, and avenanthramides in commercial grain products. Journal of Agricultural and Foo