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Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2005 / Cilt: 16 - Sayı: 1

Deksametazonun primer osteoblast apopitozundaki rolü: Hücresel ve moleküler mekanizmalar

Apoptosis of primary osteoblasts induced by dexamethasone: Cellular and molecular mechanisms

Nirong BAO
Jiannig ZHAO
Yurong WANG
Sujia WU
Xiaofeng ZENG
Junhua WANG
Pages
55–61
Publication Date
2022-10-18
DOI
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Results: Dexamethasone significantly decreased the survival rate of osteoblast-like cells through an apoptotic process, activated cellular caspase-3, and inhibited the activity of nuclear factor-kappa B, in a concentrationand time-dependent manner (p

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Objective & Hypothesis:Objectives: We investigated the effect of glucocorticoids on cell viability of osteoblasts and explored the cytological and molecular mechanisms of osteoblast apoptosis induced by glucocorticoids.
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Key Findings & Data:Surviving rate of osteoblasts, apoptosis rate, activity of caspase-3, and DNA binding activity of nuclear factor-kappa B were assessed using MTT-dye reduction microassay, flow cytometry, colorimetric substrate assay and electrophoretic mobility shift assay.
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Conclusion & Impact:Results: Dexamethasone significantly decreased the survival rate of osteoblast-like cells through an apoptotic process, activated cellular caspase-3, and inhibited the activity of nuclear factor-kappa B, in a concentrationand time-dependent manner (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: We investigated the effect of glucocorticoids on cell viability of osteoblasts and explored the cytological and molecular mechanisms of osteoblast apoptosis induced by glucocorticoids. Materials and methods: Dexamethasone was used to induce apoptosis of primary cultured osteoblast-like cells from skulls of suckling mouse. Surviving rate of osteoblasts, apoptosis rate, activity of caspase-3, and DNA binding activity of nuclear factor-kappa B were assessed using MTT-dye reduction microassay, flow cytometry, colorimetric substrate assay and electrophoretic mobility shift assay. The surviving rates of osteoblasts and fibroblasts were also compared to assess the differences in dexamethasone-induced effects on cells. Results: Dexamethasone significantly decreased the survival rate of osteoblast-like cells through an apoptotic process, activated cellular caspase-3, and inhibited the activity of nuclear factor-kappa B, in a concentrationand time-dependent manner (p

Özet

Amaç: Glikokortikoidlerin osteoblast hücrelerinin canlılığı üzerine etkisi incelendi ve osteoblast apopitozu sürecinde hücresel ve moleküler mekanizmalardaki rolü araştırıldı. Gereç ve yöntem: Deksametazon, yenidoğan farelerin kranyal kemiklerinden elde edilen primer kültürde üretilen osteoblast-benzeri hücrelerde apopitoz oluşturmak amacıyla kullanıldı. Osteoblastların canlı kalma ve apopitoz oranları, caspase-3 etkinliği ve nükleer faktör-kappa B'nin DNA bağlanma aktivitesi MTT-boya redüksiyonu mikrotesti, akım sitometrisi, kolorimetrik substrat testi ve elektroforetik mobilite şift testi ile ölçüldü. Deksametazonun hücrelere etkisindeki farklılıkları değerlendirmek amacıyla osteoblast ve fibroblastlann canlı kalma oranlan karşılaştırıldı. Bulgular: Deksametazonun, yoğunluk ve zamana bağlı olarak, apopitoz sürecinde osteoblast benzeri hücrelerin canlı kalma oranını anlamlı derecede düşürdüğü, hücresel caspase-3 aktivasyonunu artırdığı ve nükleer faktör-kappa B etkinliğini baskıladığı gözlendi (p

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Cite This Article

BAO, N., ZHAO, J., WANG, Y., WU, S., ZENG, X., WANG, J. (2022). Deksametazonun primer osteoblast apopitozundaki rolü: Hücresel ve moleküler mekanizmalar. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 16 - Sayı: 1, 55–61. https://ajindex.com/articles/498852

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References (11)

  1. Laan RF, Buijs WC, van Erning LJ, Lemmens JA, Corstens FH, Ruijs SH, et al. Differential effects of glu¬cocorticoids on cortical appendicular and cortical ver¬tebral bone mineral content. Calcif Tissue Int 1993; 52:5-9
  2. Gohel A, Gronowicz G. Glucocorticoids induce apop¬tosis in osteoblasts by the regulation of Bcl-2, Bax and other cell cycle factors. J Bone Miner Res 1997;12: S284
  3. Weinstein RS, Jilka RL, Parfitt AM, Manolagas SC. Inhibition of osteoblastogenesis and promotion of apoptosis of osteoblasts and osteocytes by glucocorticoids. Potential mechanisms of their deleterious effects on bone. J Clin Invest 1998;102:274-82
  4. Cidlowski JA, King KL, Evans-Storms RB, Montague JW, Bortner CD, Hughes FM Jr. The biochemistry and molecular biology of glucocorticoid-induced apoptosis in the immune system. Recent Prog Horm Res 1996;51:457-90
  5. Gronowicz GA, McCarthy MB. Glucocorticoids inhibit the attachment of osteoblasts to bone extracellular matrix proteins and decrease beta 1-integrin levels. Endocrinology 1995;136:598-608
  6. Hakem R, Hakem A, Duncan GS, Henderson JT, Woo M, Soengas MS, et al. Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 1998; 94:339-52
  7. Kuida K, Zheng TS, Na S, Kuan C, Yang D, Karasuyama H, et al. Decreased apoptosis in the brain and prema¬ture lethality in CPP32-deficient mice. Nature 1996; 384:368-72
  8. Herrmann JL, Beham AW, Sarkiss M, Chiao PJ, Rands MT, Bruckheimer EM, et al. Bcl-2 suppresses apoptosis resulting from disruption of the NF-kappa B survival pathway. Exp Cell Res 1997;237:101-9
  9. Wang W, Wykrzykowska J, Johnson T, Sen R, Sen J. A NF-kappa B/c-myc-dependent survival pathway is targeted by corticosteroids in immature thymocytes. J Immunol 1999;162:314-22
  10. Mayo MW, Wang CY, Cogswell PC, Rogers-Graham KS, Lowe SW, Der CJ, et al. Requirement of NF-kappa B activation to suppress p53-independent apoptosis induced by oncogenic Ras. Science 1997; 278:1812-5
  11. Chae H, Chae S, Park N, Bang B, Cho S, Kim J, et al. Pyrrolidine dithiocarbamate inhibits serum-induced NF-kappa B activation and induces apoptosis in ROS 17/2.8 osteoblasts. Int Immunopharmacol 2001;1: 255-63.