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Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2017 / Cilt: 28 - Sayı: 1

Teikoplanin, N-asetil sistein veya bunların kombinasyonunu içeren kemik çimentosunun Stafilokokkus aureus biyofilm oluşumuna etkileri: İn vitro bir çalışma

Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study

Hasan GÖÇER
Mehmet Emin ÖNGER
Nevzat DABAK
Dilek EMİR
Pages
13–18
Publication Date
2022-10-18
DOI
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Significant difference was found between group 1 and all other groups (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: This study aims to demonstrate the antibiofilm effects of teicoplanin alone, N-acetyl cysteine (NAC) alone, or combination of both compounds when mixed with bone cement.Materials and methods: A total of four groups were formed by using six cement samples in each, prepared with bone cement having different contents in each group.
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Key Findings & Data:Group 1 (control group): cement alone without any drugs added.
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Conclusion & Impact:Significant difference was found between group 1 and all other groups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: This study aims to demonstrate the antibiofilm effects of teicoplanin alone, N-acetyl cysteine (NAC) alone, or combination of both compounds when mixed with bone cement.Materials and methods: A total of four groups were formed by using six cement samples in each, prepared with bone cement having different contents in each group. Group 1 (control group): cement alone without any drugs added. Group 2: 40 g cement, 400 mg teicoplanin. Group 3: 40 g cement, 6 g NAC. Group 4: 40 g cement, 6 g NAC, 400 mg teicoplanin. All cement samples were infected with Staphylococcus aureus for 48 hours at 36.5 °C. Bacterial colonies were then counted by serial dilution method. Bacteria were counted using scanning electron microscopic (SEM) images.Results: Counts of bacteria colonies were 5.83±1.60 [mean colony forming unit (cfu) x 105±standard deviation (SD)] in group 1, 0.12±0.56 in group 2, 0.11±0.65 in group 3, and 0.01±0.001 in group 4. Significant difference was found between group 1 and all other groups (p

Özet

Amaç: Bu çalışmada sadece teikoplaninin, sadece N asetil sistein (NAC)'in veya her iki bileşenin kombinasyonunun kemik çimentosuyla karıştırılmasının antibiyofilm etkileri gösterildi.Gereç ve yöntemler: Her grupta farklı içeriğe sahip kemik çimentosu ile hazırlanan altışarlı çimento kalıplar kullanılarak toplam dört grup oluşturuldu. Grup 1 (kontrol grup): Herhangi bir ilaç katılmaksızın sadece çimento. Grup 2: 40 g çimento, 400 mg teikoplanin. Grup 3: 40 g çimento, 6 g NAC. Grup 4: 40 g çimento 6 g NAC, 400 mg teikoplanin. Tüm çimento kalıpları Stafilokokkus aureus ile 48 saat 36.5 °C'de enfekte edildi. Bakteri kolonileri seri dilüsyon yöntemiyle sayıldı. Bakteriler taramalı elektron mikroskobik (TEM) görüntüler kullanılarak sayıldı.Bulgular: Bakteri kolonisi sayıları grup 1'de 5.83±1.60 [ortalama koloni oluşturan birim (kob) x 105±standart sapma (SS)], grup 2'de 0.12±0.56, grup 3'te 0.11±0.65 ve grup 4'te 0.01±0.001 idi. Grup 1 ile tüm diğer gruplar arasında (p

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Cite This Article

GÖÇER, H., ÖNGER, M. E., DABAK, N., EMİR, D. (2022). Teikoplanin, N-asetil sistein veya bunların kombinasyonunu içeren kemik çimentosunun Stafilokokkus aureus biyofilm oluşumuna etkileri: İn vitro bir çalışma. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 28 - Sayı: 1, 13–18. https://ajindex.com/articles/498465

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