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Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2018 / Cilt: 29 - Sayı: 2

Antibiotic release and antibacterial efficacy in cement spacers and cement beads impregnated with different techniques: In vitro study

Farklı yöntemlerle hazırlanan boşluk doldurucu ve çimento boncuklarının antibiyotik salınımı ve antibakteriyel etkinliği: In vitro çalışma

Sinan ÇAVUN
Tufan KALELİ
Namık ŞAHİN
Cüneyt ÖZAKIN
Teoman ATICI
Pages
71–78
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Inhibition zone analyses of agar and disk diffusion tests revealed significant differences between several groups (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: This study aims to compare the antibiotic release and biological effectiveness of bead type and articulating spacers of different cement types with antibiotics added at alternative phases of cement preparation.
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Key Findings & Data:Results: All groups showed initial antibiotics surge in the first week, which was 2 to 4 times more evident in the beads group.
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Conclusion & Impact:Inhibition zone analyses of agar and disk diffusion tests revealed significant differences between several groups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: This study aims to compare the antibiotic release and biological effectiveness of bead type and articulating spacers of different cement types with antibiotics added at alternative phases of cement preparation. Materials and methods: Four gram vancomycin was added into two types of antibiotic-free cement (Simplex®, Biomet®) with similar viscosity and also gentamycin-containing cement (Refobacin®). Prepared specimens were used to create cement beads and articulating hip spacers, making a total of six different groups. Two alternative groups were formed by adding the Vancomycin while the cement was in dough phase. Antibiotic release and biological activity were evaluated with immunoassay techniques and agar-disk diffusion methods. Results: All groups showed initial antibiotics surge in the first week, which was 2 to 4 times more evident in the beads group. Antibiotic release and change in release rate were significantly different between Simplexalternative and Simplex, Biomet, Refobacin-beads, and between Biometspacer and Refobacin-beads groups (p0.05). Biomet cement showed larger diffusion inhibition zone in agar. There was no difference between biological activity of the bead and mobile designs of the Biomet brand (p>0.05). Inhibition zone analyses of agar and disk diffusion tests revealed significant differences between several groups (p

Özet

Amaç: Bu çalışmada çeşitli kemik çimentolarına farklı evrelerde eklenen antibiyotiklerle hazırlanan boncuk tipli ve eklem boşluk dolduruculardan antibiyotik salınımı ve biyolojik etkinliği karşılaştırıldı. Gereç ve yöntemler: Benzer viskoziteye sahip iki tip antibiyotiksiz çimentoya (Simplex®, Biomet®) ve gentamisin içeren çimentoya (Refobacin®) 4 gram vankomisin ilave edildi. Çimento boncuklarını ve kalça boşluk doldurucularını oluşturmak için hazırlanan örneklerden toplam altı farklı grup oluşturuldu. Çimento hamur fazında iken vankomisin eklenerek de iki alternatif grup oluşturuldu. Antibiyotik salınımı ve biyolojik aktivite immunoassay teknikleri ve agar-disk difüzyon yöntemleri ile değerlendirildi. Bulgular: Boncuk grubunda 2-4 kat daha fazla olmak üzere tüm gruplarda ilk haftada hızlı antibiyotik salınımı görüldü. Simplexalternatif ile Simplex, Biomet, Refobacin-zincir ve Biomet-boşluk doldurucu ile Refobacin-zincir grupları arasında antibiyotik salınımı ve salınma hızındaki değişim anlamlı olarak farklıydı (p0.05). Biomet çimento, agarda daha büyük difüzyon inhibisyon bölgesi gösterdi. Biomet çimento ile hazırlanan boncuk ve hareketli boşluk doldurucularda biyolojik etkinlik farkı yoktu (p>0.05). Agar ve disk difüzyon inhibisyon zonları gruplar arasında anlamlıydı (p

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Cite This Article

ÇAVUN, S., KALELİ, T., ŞAHİN, N., ÖZAKIN, C., ATICI, T. (2022). Antibiotic release and antibacterial efficacy in cement spacers and cement beads impregnated with different techniques: In vitro study. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 29 - Sayı: 2, 71–78. https://ajindex.com/articles/498453

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