SavedAdd your journal

Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2019 / Cilt: 30 - Sayı: 3

Icariin promotes early and late stages of fracture healing in rats

İkariin sıçanlarda kırık iyileşmesinin erken ve geç evrelerini destekler

Perihan GÜRBÜZ
Kaan GÜRBÜZ
Mükerrem Betül YERER
Mehmet HALICI
Pages
282–288
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Activities of superoxidedismutase (SOD) and glutathione peroxidase (GPx) weremeasured in the peripheral blood in addition to glutathione(GSH) and myeloperoxidase (MPO) levels to investigate theeffects of icariin on the oxidant-antioxidant systems.Results: Radiological bone mineral density measurementsand histopathological findings revealed that icariin improvedall these parameters in the two healing periods tested.Superoxide dismutase activity decreased in association withlocal icariin application to the fractured side whereas GPxand GSH increased and MPO remained unchanged.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: This study aims to evaluate the effects of locallyapplied icariin on bone fracture healing in femur fracturedrat model.Materials and methods: The study included 64 maleSprague-Dawley rats (mean age 6 months; weighing, 280-490 g)in eight main study groups.
📊
Key Findings & Data:Activities of superoxidedismutase (SOD) and glutathione peroxidase (GPx) weremeasured in the peripheral blood in addition to glutathione(GSH) and myeloperoxidase (MPO) levels to investigate theeffects of icariin on the oxidant-antioxidant systems.Results: Radiological bone mineral density measurementsand histopathological findings revealed that icariin improvedall these parameters in the two healing periods tested.Superoxide dismutase activity decreased in association withlocal icariin application to the fractured side whereas GPxand GSH increased and MPO remained unchanged.
💡
Conclusion & Impact:Icariinincreased the GPx and GSH levels which are responsible fromscavenging hydroxyl radical and hydrogen peroxide.Conclusion: Locally administered icariin to the fractureaccelerated bone healing by reducing the oxidative stress.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: This study aims to evaluate the effects of locallyapplied icariin on bone fracture healing in femur fracturedrat model.Materials and methods: The study included 64 maleSprague-Dawley rats (mean age 6 months; weighing, 280-490 g)in eight main study groups. Fracture healing process and levelwere evaluated with radiography, histopathology and dualenergy X-ray absorptiometry to investigate the effects oflocal administration of icariin at varying doses, which is anexogenous osteo-inductive substance. Activities of superoxidedismutase (SOD) and glutathione peroxidase (GPx) weremeasured in the peripheral blood in addition to glutathione(GSH) and myeloperoxidase (MPO) levels to investigate theeffects of icariin on the oxidant-antioxidant systems.Results: Radiological bone mineral density measurementsand histopathological findings revealed that icariin improvedall these parameters in the two healing periods tested.Superoxide dismutase activity decreased in association withlocal icariin application to the fractured side whereas GPxand GSH increased and MPO remained unchanged. Icariinincreased the GPx and GSH levels which are responsible fromscavenging hydroxyl radical and hydrogen peroxide.Conclusion: Locally administered icariin to the fractureaccelerated bone healing by reducing the oxidative stress.

Özet

Amaç: Bu çalışmada femur kırıklı sıçan modelinde lokal olarak uygulanan ikariinin kemik kırığı iyileşmesi üzerine etkileri değerlendirildi. Gereç ve yöntemler: Çalışmaya sekiz ana çalışma grubunda 64 erkek Sprague-Dawley sıçan (ort. yaş 6 ay; ağırlık, 280-490 g) dahil edildi. Bir ekzojen osteo-indüktif madde olan ikariinin lokal olarak değişen dozlarda uygulanmasının kırık üzerine etkilerini araştırmak için kırık iyileşme süreci ve düzeyi radyografi, histopatoloji ve çift enerjili X-ışını absorbsiyometrisi ile değerlendirildi. İkariinin oksidanantioksidan sistemler üzerindeki etkilerini araştırmak için glutatyon (GSH) ve miyeloperoksidaz (MPO) düzeylerine ek olarak periferal kanda süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) aktiviteleri ölçüldü. Bulgular: Radyolojik kemik mineral yoğunluğu ölçümleri ve histopatolojik bulgular, ikariinin test edilen iki iyileşme döneminde tüm bu parametreleri geliştirdiğini ortaya koydu. Süperoksit dismutaz aktivitesi kırıklı tarafa lokal ikariin uygulaması ile birlikte azalırken, GPx ve GSH arttı ve MPO değişmedi. Ikariin, hidroksil radikali ve hidrojen peroksiti süpürmekten sorumlu olan GPx ve GSH düzeylerini artırdı. Sonuç: Lokal olarak kırık üzerine uygulanan ikariin oksidatif stresi azaltarak kemik iyileşmesini hızlandırdı.

🔖

Cite This Article

GÜRBÜZ, P., GÜRBÜZ, K., YERER, M. B., HALICI, M. (2022). Icariin promotes early and late stages of fracture healing in rats. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 30 - Sayı: 3, 282–288. https://ajindex.com/articles/498381

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Qin L, Zhang G, Hung WY, Shi Y, Leung K, Yeung HY, et al. Phytoestrogen-rich herb formula “XLGB” prevents OVXinduced deterioration of musculoskeletal tissues at the hip in old rats. J Bone Miner Metab 2005;23:55-61
  2. An S, Li T, Li E, Effect of kidney-tonifying herbs on ovary function and bone mass in postmenopausal women. Chin J Osteoporosis 2000;6:55-9
  3. Zhang G, Qin L, Shi Y. Epimedium-derived phytoestrogen flavonoids exert beneficial effect on preventing bone loss in late postmenopausal women: a 24-month randomized, double-blind and placebo-controlled trial. J Bone Miner Res 2007;22:1072-9
  4. Hsieh TP, Sheu SY, Sun JS, Chen MH, Liu MH. Icariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2, SMAD4, and Cbfa1 expression. Phytomedicine 2010;17:414-23
  5. Fang H, Zhou L, Shen L, Zhou H, Fang Y, Fan H. Effects of icariin on the proliferation and differentiation of MC3T3-E1. Int J Clin Exp Med 2017;10:14876-82
  6. Cao H, Zhang Y, Qian W, Guo XP, Sun C, Zhang L, et al. Effect of icariin on fracture healing in an ovariectomized rat model of osteoporosis. Exp Ther Med 2017;13:2399-404
  7. Peng S, Zhang G, He Y, Wang X, Leung P, Leung K, et al. Epimedium-derived flavonoids promote osteoblastogenesis and suppress adipogenesis in bone marrow stromal cells while exerting an anabolic effect on osteoporotic bone. Bone 2009;45:534-44
  8. Sezgin EA, Atik OŞ. Are orthobiologics the next chapter in clinical orthopedics? A literature review. Eklem Hastalik Cerrahisi 2018;29:110-6
  9. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19
  10. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition 2002;18:872-9
  11. Warden SJ, Komatsu DE, Rydberg J, Bond JL, Hassett SM. Recombinant human parathyroid hormone (PTH 1-34) and low-intensity pulsed ultrasound have contrasting additive effects during fracture healing. Bone 2009;44:485-94
  12. Huo MH, Troiano NW, Pelker RR, Gundberg CM, Friedlaender GE. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. J Orthop Res 1991;9:383-90
  13. Cao H, Ke Y, Zhang Y, Zhang CJ, Qian W, Zhang GL. Icariin stimulates MC3T3-E1 cell proliferation and differentiation through up-regulation of bone morphogenetic protein-2. Int J Mol Med 2012;29:435-9
  14. Liang W, Lin M, Li X, Li C, Gao B, Gan H, et al. Icariin promotes bone formation v