In this .work, we present HPLC separation of $alpha$-galactosidase partially purified from watermelon (Citrullus vulgaris).
📌 3 Key Takeaways
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Objective & Hypothesis:Galactosidase ( $alpha$-D-galactosyl galactohydrolyse, EC 3.2.1.22), hydrolyses terminal non-reducing $alpha$-D-galactose residues in $alpha$-galactosides and galactose containing oligosaccharides, such as; melibiose and râffinose. $alpha$-D-galactosidase is widely distributed in nature and has been isolated and purified with different tecniques from animal, tissues, plants and microorganisms.
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Key Findings & Data:Galactosidase ( $alpha$-D-galactosyl galactohydrolyse, EC 3.2.1.22), hydrolyses terminal non-reducing $alpha$-D-galactose residues in $alpha$-galactosides and galactose containing oligosaccharides, such as; melibiose and râffinose. $alpha$-D-galactosidase is widely distributed in nature and has been isolated and purified with different tecniques from animal, tissues, plants and microorganisms.
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Conclusion & Impact:In this .work, we present HPLC separation of $alpha$-galactosidase partially purified from watermelon (Citrullus vulgaris).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
Galactosidase ( $alpha$-D-galactosyl galactohydrolyse, EC 3.2.1.22), hydrolyses terminal non-reducing $alpha$-D-galactose residues in $alpha$-galactosides and galactose containing oligosaccharides, such as; melibiose and râffinose. $alpha$-D-galactosidase is widely distributed in nature and has been isolated and purified with different tecniques from animal, tissues, plants and microorganisms. In this .work, we present HPLC separation of $alpha$-galactosidase partially purified from watermelon (Citrullus vulgaris).
Özet
$alpha$ Galaktosidaz ( $alpha$-D-galaktozil galaktohidrolaz, EC 3.2.1.22), $alpha$-galaktozidlerdeki ve melibioz, rafinoz gibi galaktoz içeren oligosakkaritlerdeki indirgen olmayan terminal $alpha$-D-galaktoz birimlerini hidrolizler. $alpha$-Galaktosidaz doğada oldukça yaygın olarak bulunur ve hayvansal dokulardan, bitkilerden ve mikroorganizmalardan çeşitli teknikler kullanılarak izole edilerek saflaştırılmıştır. Bu çalışmada, karpuzdan (Citrullus vulgaris) kısmi saflaştırılan $alpha$-galaktosidazın HPLC ile ayrılması verilmiştir.
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Cite This Article
TELEFONCU, A., ZİHNİOĞLU, F., ÖNAL, S. (2022). Karpuz (Citrullus vulgaris) $alpha$- galactosidazının HPLC ile ayrılması. Ege University Journal of The Faculty of Science, Cilt: 22 - Sayı: 1, 29–39. https://ajindex.com/articles/496208