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Journals / Ege Tıp Dergisi / 2020 / Cilt: 59 - Sayı: 1

Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation

Maternal uterin arter ligasyon modeli uygulanan fetal sıçanlarda kondrogenez ve osteogenezin Wnt/β-Catenin, S100 immünoekspresyon ve histomorfometri ile değerlendirilmesi

Ayşegül Uysal
Serap Uslu
Ümit İnce
Gülçin Başdemir
Arzu İrban
Fatih Oltulu
Gülperi Öktem
Kenan Demir
Pages
39–46
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The expressions of βcatenin and S100 immunohistochemically were evaluated in the growth plate.Results: Thicknesses of growth plate (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: The aim of this study is to investigate the effects of intrauterine growth retardation depending onmaternal uterine artery ligation model, Wnt/β-catenin and S100 expression immunohistochemistry andhistomorphometrically on growth plate and bone tissue of fetal rats.Materials and Methods: Maternal rats were randomly divided into 3 groups (n=5).
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Key Findings & Data:No surgery oranesthesia were applied in control group.
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Conclusion & Impact:The expressions of βcatenin and S100 immunohistochemically were evaluated in the growth plate.Results: Thicknesses of growth plate (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: The aim of this study is to investigate the effects of intrauterine growth retardation depending onmaternal uterine artery ligation model, Wnt/β-catenin and S100 expression immunohistochemistry andhistomorphometrically on growth plate and bone tissue of fetal rats.Materials and Methods: Maternal rats were randomly divided into 3 groups (n=5). No surgery oranesthesia were applied in control group. Bilaterally the maternal uterine arteries were ligated ongestational day 18 in experimental group. Although all surgical procedures were performed in shamgroup, the uterine artery ligation were not made. Fetuses were taken on gestational day 20,thicknesses of growth plate and zones, trabecular number and thickness and cortical thickness wereevaluated with the histomorphometrically in samples from left proximal tibia. The expressions of βcatenin and S100 immunohistochemically were evaluated in the growth plate.Results: Thicknesses of growth plate (p

Özet

Amaç: Bu çalışmanın amacı, sıçanlarda maternal uterin arter ligasyonuna bağlı olarak gelişen intrauterin büyüme geriliği modelinde büyüme plağı ve kemik dokuda Wnt/β-catenin ve S100 ekspresyonlarının immünohistokimyasal ve histomorfometrik olarak değerlendirilmesidir. Gereç ve Yöntem: Gebe sıçanlar üç gruba ayrıldı (n=5). Kontrol grubuna cerrahi veya anestezi uygulanmadı. Deney grubunda, gebelik 18. gününde maternal uterin arterlere bilateral ligasyon yapıldı. Sham grubuna uterin arter ligasyonu dışındaki tüm cerrahi işlemler uygulandı. Fetuslar gebeliğin 20. gününde alındı, sol proksimal tibiadan büyüme plağı ve zonlarının kalınlıkları, trabeküler sayı ve kalınlıkları, kortikal kalınlıklar histomorfometrik olarak ölçüldü. Büyüme plağında β-catenin ve S100 ekspresyonları immunohistokimyasal olarak değerlendirildi. Bulgular: Deney grubunda büyüme plağı (p

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Cite This Article

Uysal, A., Uslu, S., İnce, Ü., Başdemir, G., İrban, A., Oltulu, F., Öktem, G., Demir, K. (2022). Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation. Ege Tıp Dergisi, Cilt: 59 - Sayı: 1, 39–46. https://ajindex.com/articles/496119

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References (14)

  1. Provot S, Schipani E. Fetal growth plate a developmental model of cellular adaptation to hypoxia. Ann N Y Acad Sci 2007; 1117: 26-39
  2. Uysal A, Oktem G, Yılmaz Y, Uslu S, Aktug H, Yurtseven M. Quantitative immunohistochemical analysis of nitric oxide synthases and apoptosis regulator proteins in the fetal rat brain following maternal uterine artery ligation. Int J Neurosci 2008; 118 (6): 891-901
  3. Ergaz Z, Avgil M, Ornoy A. Intrauterine growth restriction-etiology and consequences: what do we know about the human situation and experimental animal models? Reprod Toxicol. 2005; 20 (3): 301-22
  4. Cetin I, Alvino G. Intrauterine growth restriction: Implications for placental metabolism and transport. A review. Placenta 2009; 30 (23): 77-82
  5. Tomaszewska E, Dobrowolski P, Wydrych J. Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone. J Physiol Pharmacol 2012; 63 (5): 547-54
  6. Vortkamp A, Pathi S, Peretti GM, Carusob E M, Zaleskeb D J, Tabina C J. Recapitulation of signals regulating embryonic bone formation during postnatal growth and in fracture repair. Mechanisms of Development 1998; 71 (1-2): 65-76
  7. Erenpreisa J, Roach HI. Aberrations of cell cycle and cell death in normal development of the chick embryo growth plate. Mech Ageing Dev 1999; 108 (3): 227-38
  8. DeLise AM, Fischer L, Tuan RS. Cellular interactions and signaling in cartilage development. Osteoarthritis and Cartilage 2000; 8 (5): 309-34
  9. Tamamura Y, Otani T, Kanatani N et al. Developmental regulation of Wnt/β-catenin signals are required for growth plate assembly, cartilage integrity, and endochondral ossification. J Biol Chem 2005; 280 (19): 19185-95
  10. Ornitz DM, Marie PJ. FGF signaling pathways in endochondral and intramembranous bone development and human genetic disease. Genes Dev 2002; 16 (12): 1446-65
  11. Lee SH, Kim JY, Kim H et al. Amomum villosum induces longitudinal bone growth in adolescent female rats. J Tradit Chin Med 2012; 32 (3):453-8
  12. Park H, Kong SY, Kim HW, Yang IH. Altered cellular kinetics in growth plate according to alterations in weight bearing. Yonsei Med J 2012; 53 (3): 618-24
  13. Musumeci G, Castrogiovanni P, Loreto C, Castorina S, Pichler K, Weinberg A M. Post-traumatic caspase-3 expression in the adjacent areas of growth plate injury site: a morphological study. Int J Mol Sci 2013; 14 (8): 15767-84
  14. Chen Y, Whetstone HC, Li