Journals / Ege Tıp Dergisi / 2021 / Cilt: 60 - Sayı: 1

Amniotic fluid microbiome in asymptomatic pregnants at second trimester

İkinci trimesterde asemptomatik gebeliklerde amniyotik sıvı mikrobiyomu

Pages
83–89
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This is a preliminary study; therefore, larger studies and targeted broad range molecular methods are needed to find the variety of the possible flora of the amniotic fluid.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: The purpose of this prospective cohort study is to evaluate the possible microbiome of the amniotic cavity in the second trimester in asymptomatic pregnant women by the culture-based technique.
📊
Key Findings & Data:Materials and Methods: This prospective cohort study was conducted in Gaziantep University Gynecology and Obstetrics Clinic between October 2017 and November 2019. 100 pregnant women who had amniocentesis for genetic screening in the fetus, and who had no complaints or clinical symptoms of vaginal or chorioamnionitis infection, were included in the study.
💡
Conclusion & Impact:This is a preliminary study; therefore, larger studies and targeted broad range molecular methods are needed to find the variety of the possible flora of the amniotic fluid.
🔬Design:Research Article
📍Scope:2017 Verileri
⚙️Variables:Domain Parameters

Abstract

Aim: The purpose of this prospective cohort study is to evaluate the possible microbiome of the amniotic cavity in the second trimester in asymptomatic pregnant women by the culture-based technique. Materials and Methods: This prospective cohort study was conducted in Gaziantep University Gynecology and Obstetrics Clinic between October 2017 and November 2019. 100 pregnant women who had amniocentesis for genetic screening in the fetus, and who had no complaints or clinical symptoms of vaginal or chorioamnionitis infection, were included in the study. While culture tests were performed by using the amniotic fluids of these pregnant women, glucose and leukocyte levels of their amniotic fluids were also measured. At the same time, culture and gram staining analyses were performed by collecting vaginal swab specimens from the patients. Results: 12 patients (12%, 95% confidence interval, 0 to 41%) had positive amniotic fluid culture results. The most observed bacteria were E. coli (5%). No findings of clinical infection were observed in the patients with positive amniotic fluid culture results. These patients delivered healthy babies with no complications. Conclusion: The amniotic fluid has its own microbiome, and the vaginal flora plays a role in the formation of this microbiome. This is a preliminary study; therefore, larger studies and targeted broad range molecular methods are needed to find the variety of the possible flora of the amniotic fluid.

Özet

Amaç: Bu prospektif kohort çalışmasının amacı, ikinci trimesterde asemptomatik gebelerde amniyotik kavitenin olası mikrobiyomunu kültür temelli teknikle değerlendirmektir. Gereç ve Yöntem: Bu prospektif kohort çalışma, Gaziantep Üniversitesi Kadın Hastalıkları ve Doğum Kliniği'nde Ekim 2017- Kasım 2019 tarihleri arasında gerçekleştirildi. Fetüste genetik tarama için amniyosentez yapılan, vajinal enfeksiyon veya koryoamniyonite dair klinik semptomu olmayan 100 gebe kadın çalışmaya dahil edildi. Bu gebelerin amniyotik sıvıları kullanılarak kültür testleri yapılırken, amniyotik sıvılarının glikoz ve lökosit düzeyleri de ölçüldü. Aynı zamanda hastalardan vajinal sürüntü örnekleri alınarak kültür ve gram boyama analizleri yapıldı. Bulgular: 12 hastada (%12, %95 güven aralığı, %0 ile %41) amniyotik sıvı kültürü pozitif çıktı. En sık görülen bakteri E. coli idi (%5). Amniyotik sıvı kültürü sonucu pozitif olan hastalarda klinik enfeksiyon bulgusu izlenmedi. Bu hastalar hiçbir komplikasyon olmaksızın sağlıklı bebekler doğurdu. Sonuç: Amniyotik sıvının kendi mikrobiyomu vardır ve vajinal flora bu mikrobiyomun oluşumunda rol oynar. Bu bir ön çalışmadır; bu nedenle, amniyotik sıvının olası florasının çeşitliliğini bulmak için daha büyük çalışmalara ve geniş kapsamlı moleküler yöntemlere ihtiyaç vardır.

🔖

Cite This Article

KARUSERCİ, Ö. K., ZER, Y., SUCU, S., İŞBİLEN, E., BADEMKIRAN, H., TAŞDEMİR, H. (2022). Amniotic fluid microbiome in asymptomatic pregnants at second trimester. Ege Tıp Dergisi, Cilt: 60 - Sayı: 1, 83–89. https://ajindex.com/articles/496078

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Romero R, Espinoza J, Chaiworapongsa T, Kalache K. Infection and prematurity and the role of preventive strategies. Semin Neonatol 2002; 7 (4): 259-74
  2. Harris JW, Brown JH. The bacterial content of the uterus at cesarean section. AJOG 1927; 13 (2): 133-43
  3. DiGiulio DB. Diversity of microbes in amniotic fluid. Semin Fetal Neonat M 2012;17 (1): 2-11
  4. Zhou X, Brotman RM, Gajer P, et al. Recent advances in understanding the microbiology of the female reproductive tract and the causes of premature birth. Infect Dis Obstet Gynecol. 2010
  5. Aagaard K, Ma J, Antony KM, Ganu R, Petrosino J, Versalovic J. The placenta harbors a unique microbiome. Sci Transl Med 2014; 6 (237): 237ra65-237ra65
  6. Antony KM, Ma J, Mitchell KB, Racusin DA, Versalovic J, Aagaard K. The preterm placental microbiome varies in association with excess maternal gestational weight gain. AJOG 2015; 212 (5): 653. e1-653. e16
  7. Chu D, Stewart C, Seferovic M, et al. 26: Profiling of microbiota in second trimester amniotic fluid reveals a distinctive community present in the mid trimester and predictive of the placental microbiome at parturition. AJOG 2017; 216 (1): S18-S19
  8. Gisslen T, Harris RA, Sweeney EL, et al. The placental microbiome is altered among subjects with spontaneous preterm birth with and without chorioamnionitis.2016
  9. Pelzer E, Gomez-Arango LF, Barrett HL, Nitert MD. Maternal health and the placental microbiome. Placenta 2017; 54: 30-7
  10. Lim ES, Rodriguez C, Holtz LR. Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community. Microbiome 2018; 6 (1): 1-8
  11. Montuclard B, Guibert M, Ville Y, Frydman R, Fernandez H. Does asymptomatic amniotic infection in the second trimester really exist? J Gynecol Obstet Biol Reprod 1996; 25 (2): 186-91
  12. Mändar R, Loivukene K, Ehrenberg A, et al. Amniotic fluid microflora in asymptomatic women at mid-gestation. Scand J Infect Dis 2001; 33 (1): 60-2
  13. DiGiulio DB, Romero R, Amogan HP, et al. Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation. PloS one 2008; 3 (8): e3056
  14. Gervasi MT, Romero R, Bracalente G, et al. Midtrimester amniotic fluid concentrations of interleukin-6 and interferon-gamma-inducible protein-10: evidence for heterogeneity of intra-amniotic inflammation and associations with spontaneous early ( 32 weeks) preterm delivery. J Perinat Med 2012; 40 (4): 329-43
  15. Gerber S, Vial Y, Hoh