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Journals / Ege Tıp Dergisi / 2021 / Cilt: 60 - Sayı: 4

Cell-free supernatants of lactobacilli inhibit methicilin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and carbapenem-resistant Klebsiella strains

Laktobasillerden elde edilen hücresiz süzüntülerin metisiline dirençli Staphylococcus aureus, vankomisine dirençli Enterococcus ve karbapeneme dirençli Klebsiella suşlarını inhibisyonu

Fatma KALAYCI YÜKSEK
Defne GÜMÜŞ
Derya BAYIRLI TURAN
Yaşar NAKİPOĞLU
Rıza ADALETİ
A. Mine KÜÇÜKER
Pages
332–337
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Results: All tested CFSs at all concentrations were found to inhibit growth of MRSA, VRE, CRK strains; the results were found statistically significant (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Antibiotic resistance is a major health problem.
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Key Findings & Data:Materials and Methods: Lactobacillus strains were grown in de Man Rogosa Sharpe broth; after filtration, CFSs were diluted to obtain 25, 50, and 100% concentrations.
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Conclusion & Impact:Results: All tested CFSs at all concentrations were found to inhibit growth of MRSA, VRE, CRK strains; the results were found statistically significant (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Antibiotic resistance is a major health problem. Recently, probiotics are used in the field of alternative/supportive medicine. Thus, in this study, we aimed to evaluate the in vitro inhibitory effects of four different cell-free supernatants (CFSs) of lactobacilli species (L. fermentum, L. plantarum, L. acidophilus and L. rhamnosus) on clinically isolated Methicilin-resistant Staphylococcus aureus (MRSA) strains, Vancomycin-resistant Enterococcus (VRE) strains, and Carbapenem-resistant Klebsiella (CRK) strains. Materials and Methods: Lactobacillus strains were grown in de Man Rogosa Sharpe broth; after filtration, CFSs were diluted to obtain 25, 50, and 100% concentrations. Pathogen bacteria were grown in tryptic soy broth with and without CFSs in a micro-plate. The bacterial growths were measured using spectrophotometric method after four hours of incubation at 37°C. One-way ANOVA followed by Dunnett’s multiple comparisons and Kruskal-Wallis test were used for statistical analyses. Results: All tested CFSs at all concentrations were found to inhibit growth of MRSA, VRE, CRK strains; the results were found statistically significant (p

Özet

Amaç: Antibiyotik direnci büyük bir sağlık sorunudur. Son yıllarda, probiyotikler alternatif/destekleyici tıp alanında sık kullanılmaktadır. Bu nedenle, çalışmamızda, dört farklı lactobacillus türünün (L. fermentum, L. plantarum, L. acidophilus ve L. rhamnosus) hücresiz süzüntülerinin (cell-free supernatant, CFS) etken olarak izole edilen metisiline dirençli Staphylococcus aureus (MRSA), vankomisine dirençli Enterococcus (VRE) ve karbapeneme dirençli Klebsiella (KRK) suşlarının üremeleri üzerine inhibitör etkilerini in vitro saptamayı amaçladık. Gereç ve Yöntem: Lactobacillus suşları de Man Rogosa Sharpe sıvı besiyerinde üretilmiştir; hücresiz süzüntüler %25, 50 ve 100’lük konsantrasyonları elde etmek için sulandırılmıştır. Patojen bakteriler mikroplaklarda, farklı konsantrasyonlarda CFS içeren ve içermeyen triptik soy buyyonda üretilmişlerdir. Üremeler, 37°C’deki dört saatlik inkübasyon sonunda spektrofotometrik yöntemle ölçülmüştür. İstatistiksel analizler, one-way ANOVA kullanılarak, Dunnett’in çoklu karşılaştırma testi ve Kruskal- Wallis test ile gerçekleştirilmiştir. Bulgular: İncelenen tüm CFS konsantrasyonlarının MRSA, VRE ve KRK suşlarının üremelerini inhibe ettiği saptanmış; sonuçlar istatistiksel olarak anlamlı bulunmuştur (p

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Cite This Article

YÜKSEK, F. K., GÜMÜŞ, D., TURAN, D. B., NAKİPOĞLU, Y., ADALETİ, R., KÜÇÜKER, A. M. (2022). Cell-free supernatants of lactobacilli inhibit methicilin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and carbapenem-resistant Klebsiella strains. Ege Tıp Dergisi, Cilt: 60 - Sayı: 4, 332–337. https://ajindex.com/articles/496052

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References (14)

  1. Wright GD & Sutherland AD. New strategies for combating multidrug-resistant bacteria. Trends Mol Med 2007; 13 (6): 260-7
  2. Van Duin D & Paterson DL. Multidrug-resistant bacteria in the community: trends and lessons learned. Infect Dis Clin 2016; 30 (2): 377-90
  3. Falagas ME & Bliziotis IA. Pandrug-resistant Gram-negative bacteria: the dawn of the post-antibiotic era? Inter J Antimicrobial Agent 2007;29(6): 630-6
  4. Ah YM, Kim AJ & Lee JY. Colistin resistance in Klebsiella pneumoniae. Inter J Antimicrobial Agent 2014; 44 (1): 8-15
  5. Cattoir V & Leclercq R. Twenty-five years of shared life with vancomycin-resistant enterococci: is it time to divorce? J Antimicro Chemother 2013; 68 (4): 731-42
  6. Voss A, Milatovic D, Wallrauch-Schwarz C, Rosdahl VT & Braveny I. Methicillin-resistant Staphylococcus aureus in Europe. Euro J Clin Microbiol Infect Dis 1994; 13 (1): 50-5
  7. Brunel AS & Guery B. Multidrug resistant (or antimicrobial-resistant) pathogens-alternatives to new antibiotics? Swiss Med Weekly 2017; 147
  8. Oelschlaeger TA. Mechanisms of probiotic actions—a review. Int J Med Microbiol 2010; 300: 57–62
  9. Aween MM, Hassan Z, Muhialdin BJ, Eljamel YA, Al Mabrok ASW & Lani MN. Antibacterial activity of lactobacillus acidophilus strains isolated from honey marketed in malaysia against selected multiple antibiotic resistant (mar) gram positive bacteria. J Food Sci 2012; 77 (7): M364-M371
  10. Bhola J & Bhadekar R. Invitro synergistic activity of lactic acid bacteria against multi-drug resistant staphylococci. BMC Compl Alter Med 2019; 19(1): 70
  11. Chen CC, Lai CC, Huang HL, Huang WY, Toh HS, Weng TC,. & Tang HJ. Antimicrobial activity of Lactobacillus species against carbapenem-resistant Enterobacteriaceae. Front Microbiol 2019;10: 789
  12. El-Mokhtar MA, Hassanein KM, Ahmed AS, Gad GF, Amin MM & Hassanein OF. Antagonistic Activities of Cell-Free Supernatants of Lactobacilli Against Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae and Pseudomonas aeruginosa. Infect Drug Resis 2020; 13: 543
  13. Manzoor A, Ul-Haq I, Baig S, Qazi JI & Seratlic S. Efficacy of locally isolated lactic acid bacteria against antibiotic-resistant uropathogens. Jundishapur J Microbiol 2016; 9:(1)
  14. Naderi A, Kasra-Kermanshahi R, Gharavi S, Fooladi AAI, Alitappeh MA & Saffarian P. Study of antagonistic effects of Lactobacillus strains as probiotics on multi drug resistant (MDR) bacteria isolated from urinary tract infections (UTIs). Iranian J Basic Med Sc