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Journals / Ege Klinikleri Tıp Dergisi / 2021 / Cilt: 59 - Sayı: 3

Experimental Investigation of the Antimicrobial Efficacy of Herbal Based Bleeding Stopper Ankaferd Blood Stopper® in an Intra-abdominal Sepsis Model

Bitkisel Kaynaklı Kanama Durdurucu Ankaferd Blood Stopper® ’in Antimikrobiyal Etkinliğinin İntraabdominal Sepsis Modelinde Deneysel Olarak Araştırılması

Volkan HANCI
Kemal KARAKAYA
Elif AKTAŞ
Pages
1–6
Publication Date
2022-10-11
DOI
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Results: In the control group, ABS-CLP group, and ABS-CLP-Liver trauma group, the number of subjects with growth in abdominal aspirate and blood culture was significantly higher than in the Sham, PiperacillineTazobactam-CLP-IP and Piperacilline-Tazobactam-CLP-SC groups (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim:In this study, it was planned to investigate the in-vivo antimicrobial effect of Ankaferd Blood Stopper®(ABS) on bacterial growth in the intra-abdominal sepsis model in rats.
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Key Findings & Data:Materials and Methods:52 Wistar-Albino rats were included in the study.
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Conclusion & Impact:Results: In the control group, ABS-CLP group, and ABS-CLP-Liver trauma group, the number of subjects with growth in abdominal aspirate and blood culture was significantly higher than in the Sham, PiperacillineTazobactam-CLP-IP and Piperacilline-Tazobactam-CLP-SC groups (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim:In this study, it was planned to investigate the in-vivo antimicrobial effect of Ankaferd Blood Stopper®(ABS) on bacterial growth in the intra-abdominal sepsis model in rats. Materials and Methods:52 Wistar-Albino rats were included in the study. In the sham group, the cecum was taken out of the abdomen and then placed into the abdomen. Experimental peritonitis was created in the control group by the cecal ligation puncture (CLP) method.In the Ankaferd-CLP group, the abdomen was closed after the intraperitoneal 1ml ABS application to the rats who underwent CLP. In the Ankaferd-CLP-Liver trauma group, liver laceration was created and 1ml of ABS was applied on it, and then CLP was performed. In the Piperacilline-Tazobactam-CLP-IP group, a single intraperitoneal dose of 120 mg/kg Piperacilline-Tazobactam was administered to the rats treated with CLP. In the Piperacilline-Tazobactam-CLP-SC group, 120 mg/kg Piperacillin-Tazobactam was administered subcutaneously to the rats who underwent CLP. After 24 hours, intra-abdominal aspiration and blood samples were taken. Results: In the control group, ABS-CLP group, and ABS-CLP-Liver trauma group, the number of subjects with growth in abdominal aspirate and blood culture was significantly higher than in the Sham, PiperacillineTazobactam-CLP-IP and Piperacilline-Tazobactam-CLP-SC groups (p

Özet

Giriş: Bu deneysel çalışmada, ratlarda çekum ligasyonu-puncture yöntemi ile oluşturulan intraabdominal sepsis modelinde bakteri üremesi üzerine Ankaferd Blood Stopper® (ABS)’nin in-vivo antimikrobial etkisinin araştırılması planlanmıştır. Gereç ve Yöntem: Çalışmaya Wistar-Albino cinsi, ağırlıkları 250-300 gr arasında değişen, 52 adet erkek rat alındı. Denekler, randomize olarak sekizer rat içeren 6 ayrı gruba ayrıldı. Sham grubunda çekum karın dışına alınıp elle manuple edildikten sonra başka işlem yapılmadan karın içine yerleştirildi. Kontrol grubunda Literatürde daha tanımlandığı şekilde çekal ligasyon ponksiyon (CLP) yöntemi ile deneysel peritonit oluşturuldu. Ankaferd CLP grubunda CLP uygulanan ratlara intraperitoneal 1 ml ABS uygulamasını takiben abdomen kapatıldı. Ankaferd CLP-Karaciğer travması grubunda karaciğer laserasyon oluşturularak üzerine 1ml ABS uygulandıktan sonra CLP gerçekleştirildi. Piperacilline-Tazobactam CLP-IP grubunda CLP uygulanan ratlara intraperitoneal tek doz 120 mg/kg Piperacilline-Tazobactam uygulandı. PiperacillineTazobactam CLP-SC grubunda CLP uygulanan ratlara subkutan 120 mg/kg Piperacilline-Tazobactam tek doz olarak uygulandı. 24 saat sonra intramuskuler ketamin anestezisi altında tüm ratlara relaparotomi yapılarak batın içi aspirasyon ve kan örnekleri alındı. Gruplarda batın içi aspirasyon sıvısında, kan kültüründe üreme olan denek sayıları ve üreyen koloni sayıları belirlenip istatistik analiz yapıldı. Bulgular: Kontrol grubu, ankaferd CLP grubu ve ankaferd CLP-Karaciğer travması grubunda batın aspiratı ve kan kültüründe üreme olan denek sayıları Sham, Piperacilline-Tazobactam CLP-IP ve Piperacilline-Tazobactam CLP-SC grubundan anlamlı olarak yüksekti (p

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Cite This Article

HANCI, V., KARAKAYA, K., AKTAŞ, E. (2022). Experimental Investigation of the Antimicrobial Efficacy of Herbal Based Bleeding Stopper Ankaferd Blood Stopper® in an Intra-abdominal Sepsis Model. Ege Klinikleri Tıp Dergisi, Cilt: 59 - Sayı: 3, 1–6. https://ajindex.com/articles/495632

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