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Journals / Düzce Üniversitesi Bilim ve Teknoloji Dergisi / 2020 / Cilt: 8 - Sayı: 1

Tryptamine Biosensor Based on Amino-Functionalized Multiwalled Carbon Nanotubes, Tin Oxide Nanoparticles and Diamine Oxidase

Amin Fonksiyonlu Karbon Nanotüp, Kalay Oksit Nanopartikül ve Diamin Oksidaz Temelli Triptamin Biyosensörü

Ceren KAÇAR
Berna DALKIRAN
Pages
631–641
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Biosensor also showed high repeatability and reproducibility.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, amperometric tryptamine biosensor based on amino functionalized multiwalled carbon nanotubes (NH2-MWCNT) and tin oxide nanoparticles (SnO2) modified screen-printed carbon electrode (SPCE) was developed.
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Key Findings & Data:Diamine oxidase (DAO) enzyme was covalently immobilized onto NH2-MWCNT-SnO2/SPCE surface via (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (EDC) ve N-hydroxysuccinimide (NHS) chemistry.
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Conclusion & Impact:Biosensor also showed high repeatability and reproducibility.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Biyojenik amin · Triptamin · Biyosensör

Abstract

In this study, amperometric tryptamine biosensor based on amino functionalized multiwalled carbon nanotubes (NH2-MWCNT) and tin oxide nanoparticles (SnO2) modified screen-printed carbon electrode (SPCE) was developed. Diamine oxidase (DAO) enzyme was covalently immobilized onto NH2-MWCNT-SnO2/SPCE surface via (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (EDC) ve N-hydroxysuccinimide (NHS) chemistry. The resulting electrode surface was finally covered with Nafion in order to prevent enzyme leakage from the surface and minimize the effect of interferences. The surface morphology, electrochemical bahaviour and analytical performance of the biosensor was investigated by scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry methods. Linear working range, limit of detection and sensitivity of the developed biosensor was found to be 2.0×10-6 ‒ 2.5×10-3 M, 6.0×10-7 M ve 6.52 µA mM-1, respectively. Biosensor also showed high repeatability and reproducibility.

Özet

Bu çalışmada amino fonksiyonlu çok duvarlı karbon nanotüp (NH2-MWCNT) ve kalay oksit nanopartikül (SnO2) ile modifiye edilmiş perde baskılı karbon elektrotlara (SPCE) dayanan amperometrik triptamin biyosensörü geliştirildi. Diamin oksidaz (DAO) enzimi NH2-MWCNT-SnO2/SPCE yüzeyine N-etil-N′-(3-dimetilaminopropil) karbodiimit (EDC) ve N-hidroksi süksinimit (NHS) kullanılarak kovalent bağlama yöntemi ile immobilize edildi. Hazırlanan elektrot yüzeyi, enzimlerin yüzeyden uzaklaşmasını engellemek ve girişim etkilerini azaltmak amacıyla son olarak Nafyon ile kaplandı. Biyosensörün yüzey morfolojisi, elektrokimyasal özellikleri ve analitik performansı taramalı elektron mikroskobu (SEM), dönüşümlü voltammetri (CV), elektrokimyasal empedans spektroskopi (EIS) ve kronoamperometri yöntemleri kullanılarak incelendi. Geliştirilen biyosensör ile triptamin için elde edilen doğrusal çalışma aralığı, gözlenebilme sınırı ve duyarlık sırası ile 2,0×10-6 ‒ 2,5×10-3 M, 6,0×10-7 M ve 6,52 µA mM-1 olarak bulundu. Hazırlanan biyosensörün tekrar kullanılabilirlik ve tekrar üretilebilirliğinin oldukça iyi olduğu belirlendi.

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Cite This Article

KAÇAR, C., DALKIRAN, B. (2022). Tryptamine Biosensor Based on Amino-Functionalized Multiwalled Carbon Nanotubes, Tin Oxide Nanoparticles and Diamine Oxidase. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, Cilt: 8 - Sayı: 1, 631–641. https://ajindex.com/articles/494101

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References (1)

  1. 1] X. Xing, S. Liu, J. Yu, W. Lian ve J. Huang, “Electrochemical sensor based on molecularly imprinted film at polypyrrole-sulfonated graphene/hyaluronic acid-multiwalled carbon nanotubes modified electrode for determination of tryptamine,” Biosensors and Bioelectronics, c. 31, s. 1, ss. 277–283, 2012. [2] T. Ramon-Marquez, A. L. Medina-Castillo, A. Fernandez-Gutierrez ve J. F. Fernandez-Sanchez, “Novel optical sensing film based on a functional nonwoven nanofibre mat for an easy, fast and highly selective and sensitive detection of tryptamine in beer,” Biosensors and Bioelectronics, c. 79, ss. 600–607, 2016. [3] B. Bóka, N. Adányi, D. Virág, M. Sebela ve A. Kiss, “Spoilage Detection with Biogenic Amine Biosensors, Comparisonof Different Enzyme Electrodes,” Electroanalysis, c. 24, s. 1, ss. 181–186, 2012. [4] J. H. Mah, H. K. Han, Y. J. OH, M. G. Kim ve H. J. Hwang, “Biogenic amines in Jeotkals, Korean salted and fermented fish products,” Food Chemistry, c. 79, s. 2, ss. 239–243, 2002. [5] L. Beneduce, A. Romano, V. Capozzi, P. Lucas, L. Barnavon, B. Bach, P. Vuchot, F. Grieco ve G. Spano, “Biogenic amine in wines,” Annals of Microbiology, c. 60, s. 4, ss. 573–578, 2010. [6] H. Tatsumi ve T. Ueda, “Ion transfer voltammetry of tryptamine, serotonin, and tryptophan at the nitrobenzene/water interface,” Journal of Electroanalytical Chemistry, c. 655, s. 2, ss. 180–183, 2011. [7] X. Meng, W. Guo, X. Qin, Y. Liu, X. Zhu, M. Pei ve L. Wang, “A molecularly imprinted electrochemical sensor based on gold nanoparticles and multiwalled carbon nanotube–chitosan for the detection of tryptamine,” RSC Advances, c. 4, s. 73, ss. 38649–38654, 2014. [8] N. Innocente, M. Biasutti, M., Padovese ve S. Moret, “Determination of biogenic amines in cheese using HPLC technique and direct derivatization of acid extract,” Food Chemistry, c. 101, s. 3, ss. 1285–1289, 2007. [9] G. Sagratini, M. Fernández-Franzón, F. De Berardinis, G. Font, S. Vittori ve J. Mañes, “Simultaneous determination of eight underivatised biogenic amines in fish by solid phase extraction and liquid chromatography–tandem mass spectrometry,” Food Chemistry, c. 132, s. 1, ss. 537–543, 2012. [10] G. Favaro, P. Pastore, G. Saccani ve S. Cavalli, “Determination of biogenic amines in fresh and processed meat by ion chromatography and integrated pulsed amperometric detection on Au electrode,” Food Chemistry, c. 105, s. 4, ss. 1652–1658, 2007. [11] F. Kvasnička ve M. Voldřich, “Determination of biogenic amines by capill