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Journals / Düzce Üniversitesi Bilim ve Teknoloji Dergisi / 2021 / Cilt: 9 - Sayı: 5

Statistical Optimization of Urease Production by Bacillus B10 Strain Isolated From Kilis Soils

Kilis Topraklarından İzole Edilen Bacillus B10 Suşunun Üreaz Üretiminin İstatiksel Optimizasyonu

Hatice Aysun MERCİMEK TAKCI
Pemra BAKIRHAN
Pages
1782–1792
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Maximum urease production was observed in a medium containing 5.00% urea, 3.00% sucrose and 2.00% meat extract and the highest specific activity under optimum conditions was calculated as 1.498 ± 0.05 µmol/min/mg.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, urease producer Bacillus spp. strains were isolated from Kilis soil samples.
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Key Findings & Data:The specific activities of intracellular urease purified from strains named as B10 and B11 were 1.38±0.07 and 1.22±0.02 µmol/min/mg.
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Conclusion & Impact:Maximum urease production was observed in a medium containing 5.00% urea, 3.00% sucrose and 2.00% meat extract and the highest specific activity under optimum conditions was calculated as 1.498 ± 0.05 µmol/min/mg.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bacillus spp. · Box Behnken · üreaz

Abstract

In this study, urease producer Bacillus spp. strains were isolated from Kilis soil samples. The specific activities of intracellular urease purified from strains named as B10 and B11 were 1.38±0.07 and 1.22±0.02 µmol/min/mg. Box Behnken design using "Design Expert statistical software" (Design Expert 8.0.7) was applied to increase the urease production of B10 strain. Maximum urease production was observed in a medium containing 5.00% urea, 3.00% sucrose and 2.00% meat extract and the highest specific activity under optimum conditions was calculated as 1.498 ± 0.05 µmol/min/mg.

Özet

Bu çalışmada Kilis ili toprak örneklerinden üreaz üretici Bacillus spp. suşları izole edilmiştir. B10 ve B11 olarak isimlendirilen suşlardan saflaştırılan intraselüler üreazın spesifik aktiviteleri 1.38±0.07 ve 1.22±0.02 µmol/min/mg’dır. B10 suşunun üreaz üretimini arttırabilmek için “Design Expert statistical software” (Design Expert 8.0.7)’in kullanıldığı Box Behnken deneme planı uygulanmıştır. Maksimum üreaz üretimi %5.00 üre, %3.00 sükroz ve %2.00 et özütü içeren besi ortamında gözlenmiş ve optimum koşullardaki en yüksek spesifik aktivite 1.498±0.05 µmol/min/mg olarak hesaplanmıştır.

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Cite This Article

TAKCI, H. A. M., BAKIRHAN, P. (2022). Statistical Optimization of Urease Production by Bacillus B10 Strain Isolated From Kilis Soils. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, Cilt: 9 - Sayı: 5, 1782–1792. https://ajindex.com/articles/494031

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