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Journals / Düzce Üniversitesi Bilim ve Teknoloji Dergisi / 2022 / Cilt: 10 - Sayı: 1

Application of PCR and PLS Tools for the Simultaneous Quantification of Praziquantel and Ivermectin Binary Mixtures in Veterinary Tablets

Veteriner Tabletlerinde Prazikuantel ve İvermektinin Eş Zamanlı Ölçümü için PCR ve PLS Yöntemlerinin Uygulanması

Eda BÜKER
Erdal DİNÇ
Nagihan YAŞAR
Pages
385–397
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Assay results showed that both PCR and PLS approaches provided an opportunity for quantifying PRA and IVE in veterinary tablet formulation.

📌 3 Key Takeaways

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Objective & Hypothesis:Two chemometric calibration methods, principal component regression (PCR) and partial least squares regression (PLS) were proposed for the simultaneous spectrophotometric determination of praziquantel (PRA) and ivermectin (IVE) in a marketed veterinary formulation without using preliminary separation step.
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Key Findings & Data:UV spectra of calibration set and samples including PRA and IVE were recorded in the spectral region of 225-315 nm.
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Conclusion & Impact:Assay results showed that both PCR and PLS approaches provided an opportunity for quantifying PRA and IVE in veterinary tablet formulation.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:PCR ve PLS kalibrasyon · Eşzamanlı miktarsal ölçüm · Prazikuantel

Abstract

Two chemometric calibration methods, principal component regression (PCR) and partial least squares regression (PLS) were proposed for the simultaneous spectrophotometric determination of praziquantel (PRA) and ivermectin (IVE) in a marketed veterinary formulation without using preliminary separation step. UV spectra of calibration set and samples including PRA and IVE were recorded in the spectral region of 225-315 nm. PCR and PLS algorithms were applied to absorbance data matrix and concentration set including PRA and IVE in the linear concentration range of 20.0-160.0 µg/mL for PRA and 2.0-44.0 µg/mL for IVE. The capability of the PCR and PLS methods were validated by analyzing validation samples. Assay results showed that both PCR and PLS approaches provided an opportunity for quantifying PRA and IVE in veterinary tablet formulation.

Özet

Ticari bir veteriner formülasyonunda ön ayırma adımı kullanılmadan prazikuantel (PRA) ve ivermektinin (IVE) eş zamanlı spektrofotometrik tayini için iki kemometrik kalibrasyon yöntemi, temel bileşen regresyonu (PCR) ve kısmi en küçük kareler regresyonu (PLS) önerilmiştir. Kalibrasyon setinin UV spektrumları ve PRA ve IVE içeren numuneler 225-315 nm spektral bölgede kaydedildi. PRA için 20.0-160.0 µg/mL ve IVE için 2.0-44.0 µg/mL lineer konsantrasyon aralığında PRA ve IVE içeren absorbans veri matrisi ve konsantrasyon setine PCR ve PLS algoritmaları uygulandı. PCR ve PLS yöntemlerinin kapasitesi, doğrulama örnekleri analiz edilerek doğrulandı. Değerlendirme sonuçları, hem PCR hem de PLS yaklaşımlarının, veteriner tablet formülasyonunda PRA ve IVE'yi ölçmek için bir fırsat sağladığını gösterdi.

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Cite This Article

BÜKER, E., DİNÇ, E., YAŞAR, N. (2022). Application of PCR and PLS Tools for the Simultaneous Quantification of Praziquantel and Ivermectin Binary Mixtures in Veterinary Tablets. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, Cilt: 10 - Sayı: 1, 385–397. https://ajindex.com/articles/493830

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References (7)

  1. A. Dayan, “Albendazole, mebendazole and praziquantel. Review of non‐clinical toxicity and pharmacokinetics,” Acta Tropica, vol. 86, pp. 141–159, 2003.
  2. Eric A. Ottesen, and W. Campbell, “Ivermectin in human medicine,” Journal of Antimicrobial Chemotherapy, vol. 34, no. 2, pp. 195–203, 1994. [3]. M. S. Piantavini, F. L. Pontes, L. X. Weiss, M. M. Senab, and R. Pontarolo, “Comparison between ultraviolet and infrared spectroscopies for the simultaneous multivariate determination of pyrantel and praziquantel,” J Braz Chem Soc., vol. 26, pp. 1387-95, 2015. doi:10.5935/0103-5053.20150107 [4] H. M. Phatak, V. V. Vaidya, M. S. Phatak, and D. Rajeghadge, “A rapid high performance liquid chromatography method for simultaneous quantification of praziquantel, ivermectin and abamectin from veterinary formulations: Development, validation and application,” Int J Pharm Res Scholars, vol. 5, pp. 57-65, 2016
  3. S. Rupali, B. Shyamala, K. Kulesh, and K. J. Shailendra, “Simultaneous estimation of pyrantel pamoate, praziquantel & febantel by high performance liquid chromatography using dual wavelength,” J Appl Pharm Res., vol. 2, pp. 32-43, 2014
  4. R. Rajesh, and J. J. Jithu, “A validated RP-HPLC method for simultaneous estimation of pyrantel pamoate and praziquantel in bulk and pharmaceutical dosage form,” Int J Pharm Pharm Sci, vol. 11, pp. 62-67, 2019. doi:10.22159/ijpps.2019v11i5.30488 [7] W. Hanpitakpong, V. Banmairuroi, B. Kamanikom, A. Choemung, and K. Na-Bangchang, “A high-performance liquid chromatographic method for determination of praziquantel in plasma,” Journal of Pharmaceutical and Biomedical Analysis, vol. 36, pp. 871–876, 2004
  5. G. Vijay Kumar, B. Sravanthi, and A. Praveen, “Analytical method development and validation for ivermectin and albendazole in combine dosage form by RP-HPLC,” Int J Curr Trends Pharma Res., vol. 7, pp. 7-12, 2019
  6. B. Bhavya, P. Nagaraju, V. Mounika, and G. I. Priyadarshini, “Stability indicating RP-HPLC method development and validation for simultaneous estimation of albendazole and ivermectin in pharmaceutical dosage form,” Asian J Pharm Anal., vol. 7, pp. 6-14, 2017. doi:10.5958/2231-5675.2017.00002.3 [10] A.K. Patel, H.V. Joshi, and J.K. Patel, “Development and validation of stability indicating RP-HPLC method for estimation of ivermectin and albendazole in pharmaceutical dosage form,” Indian J. Drugs, vol. 3, pp. 57–70, 2015
  7. L. K. Vegad, B. D. Paranjape, A. D.