Journals / Aquatic Sciences and Engineering / 2019 / Cilt: 34 - Sayı: 4

The Characterization and Activity of Protease Enzyme on Different Days of Tadpoles [Pelophylax ridibundus (Pallas, 1771)]

Pages
112–115
Publication Date
2019-09-16
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results can be used as the basis for the most appropriate nutritional formulation for P. ridibundus in the larval stage.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, protease enzyme activity of Pelophylax ridibundus (Pallas, 1771) tadpoles was determined during the larval period starting from 25 Gosner stage (1960).
📊
Key Findings & Data:When the P. ridibundus tadpoles reached the 25 Gosner stage (1960) on the 22nd day, the sampling was continued until the metamorphosis was completed by sampling at 5-day intervals.
💡
Conclusion & Impact:These results can be used as the basis for the most appropriate nutritional formulation for P. ridibundus in the larval stage.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Pelophylax ridibundus · frog · acid protease

Abstract

In this study, protease enzyme activity of Pelophylax ridibundus (Pallas, 1771) tadpoles was determined during the larval period starting from 25 Gosner stage (1960). When the P. ridibundus tadpoles reached the 25 Gosner stage (1960) on the 22nd day, the sampling was continued until the metamorphosis was completed by sampling at 5-day intervals. Initial average weight and length of the tadpoles were 0.076 g and 6.42 mm, respectively. The tadpoles were fed with pellet feed the size of 2 mm and 39% crude protein content twice a day in the morning and afternoon. The differences in the protease activity between the 22nd day and the 62nd day of the metamorphosis were found statistically significant (P<0.05). It has been observed that the protease enzyme reached maximum level at the 32nd day. In the next stages, protease activity showed a tendency to increase and decrease up to metamorphosis. These results can be used as the basis for the most appropriate nutritional formulation for P. ridibundus in the larval stage.

🔖

Cite This Article

ŞEREFLİŞAN, H., ALKAYA, A., GÖKÇEK, K. (2019). The Characterization and Activity of Protease Enzyme on Different Days of Tadpoles [Pelophylax ridibundus (Pallas, 1771)]. Aquatic Sciences and Engineering, Cilt: 34 - Sayı: 4, 112–115. https://ajindex.com/articles/48069

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Albinati, R. C. B., Lima, S. L., Tafuri, M. L., & Donzele, J. L. (2000). Digestibilidade aparente de dois alimentos protéicos e três energéticos para girinos de rã-touro (Rana catesbeiana Shaw, 1802). Revista Brasileira de Zootecnia, 29(6), 2151-2156. http://www.sbz.org.br/revista/artigos/2715.pdf
  2. Albinati, R. C. B., Lima, S. L., & Donzele, J. L. (2001). Níveis de energia digestível na ração de girinos de rã-touro. Revista Brasileira de Saúde da Produção Animal, 2(2), 48-52. http://www.repositorio.ufba.br/ri/handle/ri/1840
  3. Bambozzi, A. C., Seixas-Filho, J. D., Thomaz, L. A., Oshiro, L. M. Y., Braga, L. G. T., & Lima, S. L. (2004). Efeito do fotoperíodo sobre o desenvolvimento de girinos de rã-touro (Rana catesbeiana Shaw, 1802). Revista Brasileira de Zootecnia, 1(1), 1–7.
  4. Barbosa, J. M., Silveira, A. M., & Gomide, C. A. (2005). Crescimento heterogêneo de girinos de rã-touro alimentados com diferentes rações. Pesquisa Agropecuária Brasileira, 40(10), 1015–1019.
  5. Bonneville, M. A. (1963). Fine structural changes in the intestinal epithelium of the bullfrog during metamorphosis. The Journal of cell biology, 18(3), 579–597.
  6. Bonneville, M. A., & Weinstock, M. (1970). Brush border development in the intestinal absorptive cells of Xenopus during metamorphosis. The Journal of cell biology, 44(1), 151–171.
  7. Braga, L. G. T., & Lima, S. L. (2001). Influência da temperatura ambiente no desempenho da rã-touro, Rana catesbeiana (Shaw, 1802) na fase de recria. Revista Brasileira de Zootecnia, 30(6), 1659–1663.
  8. Braga, L. G. T., Oliveira, M. D. A., Lima, W. C., & Euclydes, R. F. (2004). Atividade da tripsina em rã-touro na fase pós-metamórfica. Revista Brasileira de Zootecnia, 33(4), 821–827.
  9. Braga, L. G. T., Oliveira, M. G. A., Lima, W. C., & Euclydes, R. F. (2006). F. Atividade enzimática da lipase em rã-touro na fase pós-metamórfica. Scientia Agrícola, 63(5), 821–827.
  10. Brasford, M. M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248–254.
  11. Carmona-Osalde, C., Olvera-Novoa, M. A., Rodriguez-Serna, M., & Flores-Nava, A. (1996). Estimation of the protein requirement for bullfrog (Rana catesbeiana) tadpoles, and its effect on metamorphosis ratio. Aquaculture, 141(3-4), 223–231.
  12. Duellman, W. E., & Trueb, L. (1986).
  13. Biology of Amphibians. New York, United States of America, McGraw–Hill Press., 613 pp. Etkin, W. (1968). Hormonal control of amphibian metamorphosis. In Etki