Journals / CBÜ Beden Eğitimi ve Spor Bilimleri Dergisi / 2018 / Cilt: 13 - Sayı: 1

The Effect of Different Running Treadmill Exercises on Oxidative Stress Parameters in Rats Muscle Tissue

Farklı sürelerdeki koşu bandı egzersizinin sıçan kas dokusundaki oksidatif stres parametreleri üzerine etkisi

Pages
115–124
Publication Date
2018-06-30
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It may be suggested that the antioxidant supplementation taken with the exercises made at different times may be more effective on muscle tissue.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim of this study was planned to determine the effect of treadmill exercises made on different occasions on the oxidative stress parameters on the rat muscle tissue.
📊
Key Findings & Data:In the present study, 32 Male Albino Wistar rats weighing 280-350 grams were used.
💡
Conclusion & Impact:It may be suggested that the antioxidant supplementation taken with the exercises made at different times may be more effective on muscle tissue.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Egzersiz · Antioksidan · Serbest Radikal

Abstract

Aim of this study was planned to determine the effect of treadmill exercises made on different occasions on the oxidative stress parameters on the rat muscle tissue. In the present study, 32 Male Albino Wistar rats weighing 280-350 grams were used. Rats were administered a treadmill exercise protocol for 13 weeks, short (15 minutes, n:8), modarate (30 minutes, n:8) and long time (60 minutes, n:8) for five days. For each biochemical study, superoxide dismutase activity (SOD), malondialdehyde (MDA) levels and glutathione (GSH) levels from each supernatant were measured in duplicate in each rat muscle tissue by ELISA kits, respectively. Results from the experiments were given as mean±standard deviation, and P values below 0.05 were considered statistically significant. The significance level difference between groups was determined using the one-way analysis of variance (ANOVA) test and post-hoctests using the "LSD" technique. The control group consisted of 23,07 ± 3,38 (U/mg protein), 15 min group (19,82 ± 4,77 U/mg protein) and 30,88 ± 4,18 (U/Running was determined as SOD value of 60 min 22.15 ± 3.85 (U/mg protein). The control group consisted of 0,43 ± 0,6 (nmol/mg protein), 15 minutes group with 0,35 ± 0,3 (nmol/mg protein), 30 minutes 0,23 ± 0,6ab (nmol/mg protein), Running 60 min 0,24 ± 0,1 (nmol / mg protein), GSH value. The control group consisted of 0.19 ± 0.02 (nmol/mg protein), 15 min group of running 0.12 ± 0.03 (nmol/mg protein), running 30 min 0.14 ± 0.01 (nmol/mg protein), Running was 0,14 ± 0,01 (nmol/mg protein), GSH valuefor 60 min. As a result, it was determined that treadmill exercise performed at different times had a positive effect on the oxidative stress parameters occurring in rat muscle. It may be suggested that the antioxidant supplementation taken with the exercises made at different times may be more effective on muscle tissue.

Özet

Bu çalışmanın amacı; farklı sürelerde yapılan koşu bandı egzersizlerinin sıçan kas dokusu üzerinde meydana gelen oksidatif stres parametrelerine etkisini belirlemektir. Sunulan çalışmada ağırlıkları 280-350 gram olan Albino Wistar cinsi 32 adet erkek sıçan kullanılmıştır. Sıçanlara, 13 hafta boyunca koşu bandı egzersiz protokolü, kısa (15 dakika, n:8), orta (30 dakika, n:8) ve yüksek (60 dakika, n:8) düzeyde, beş gün arasında uygulanmıştır. Biyokimyasal çalışmalar için her süpernatanttan süperoksit dismutaz aktivitesi (SOD), malondialdehit (MDA) seviyeleri ve glutatyon (GSH) seviyeleri sırasıyla ELISA kitleriyle her bir rat kas dokusu ikişer tekrarlamalı olarak ölçülmüştür. Deneylerden elde edilen sonuçlar ortalama ± standart sapma olarak verildi ve 0.05'in altındaki P değerleri, istatistiksel açıdan anlamlı olarak kabul edilmiştir. Gruplar arası farkın önemlilik derecesi tek yönlü varyans analizi (ANOVA) testi ile ve post-hoc testlerinden “LSD” tekniği kullanılarak belirlenmiştir. Kontrol grubu 23,07±3,38 (U/mg protein), Koşu 15 dk. grubu 19,82±4,77 (U/mg protein), Koşu 30 dk. 20,88±4,18 (U/mg protein) Koşu 60 dk. 22,15±3,85 (U/mg protein) SOD değeri olarak tespit edilmiştir. Kontrol grubu 0,43±0,6 (nmol/mg protein), Koşu 15 dk. grubu 0,35±0,3 (nmol/mg protein), Koşu 30 dk. 0,23±0,6ab (nmol/mg protein), Koşu 60 dk. 0,24±0,1(nmol/mg protein), GSH değeri olarak tespit edilmiştir. Kontrol grubu 0,19±0,02 (nmol/mg protein), Koşu 15 dk. grubu 0,12±0,03 (nmol/mg protein), Koşu 30 dk. 0,14±0,01 (nmol/mg protein), Koşu 60 dk. 0,14±0,01 (nmol/mg protein), GSH değeri olarak tespit edilmiştir. Sonuç olarak farklı sürelerde yapılan koşu bandı egzersizi sıçan kas dokusunda meydana gelen oksidatif stres parametreleri üzerinde pozitif etkisinin olduğu tespit edilmiştir. Farklı sürelerde yapılan egzersizler ile birlikte alınan antioksidan desteğinin kas dokusu üzerinde daha etkili olabileceği önerilebilir.

🔖

Cite This Article

TAŞ, R., SOSLU, R., TAŞ, M., AKYÜZ, M., AKDEMİR, F. N. E. (2018). The Effect of Different Running Treadmill Exercises on Oxidative Stress Parameters in Rats Muscle Tissue. CBÜ Beden Eğitimi ve Spor Bilimleri Dergisi, Cilt: 13 - Sayı: 1, 115–124. https://ajindex.com/articles/480511

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Akdemir, F.N.E., Gülçin, İ., Karagöz, B., & Soslu, R. (2016). Quercetin protects rat skeletal muscle from ischemia reperfusion injury. Journal Of Enzyme İnhibition And Medicinal Chemistry, 31(2), 162-166.
  2. Childs, A., Jacobs, C., Kaminski, T., Halliwell, B. & Leeuwenburgh, C. (2001). Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise. Free Radical Biology and Medicine, 31, 745-53.
  3. Clarkson, P.M. (1995). Antioxidants and physical performance. Critical Reviews in Food Science and Nutrition, 35, 131-41.
  4. Cotman, C.W., & Berchtold, N.C. (2002). Exercise: a behavioral intervention to enhance brain health and plasticity. Trend Neuroscience, 25, 295–301.
  5. Cotman, C.W., & Engesser-Cesar, C. (2002). Exercise enhances and protects brain function. Exercise Sport Science Review, 30, 75–79.
  6. Çolakoğlu, S., Çolakoğlu, M., Kırkalı, M., Örmen, M., & Akan, P. (1999). E vitamini desteğinin submaksimal egzersizde oksidan stres ve dayanıklılık üzerine etkileri. Beden Eğitimi Spor Bilimleri Dergisi, 3(3). Davies, K.J.A., Qumtanılha, A.T., Brooks, G.A., & Packer, L. (1982). Free radicals and tissue damage produced by exercise. Biochemical, Biophysical Research Communications, 107, 1198- 1205. Dixon, C.B., Robertson, R.J., Goss, F.L., Timmer, J.M., Nagle, E., & Evans, R.W. (2003). Effect of resistance training status on free radical production and muscle damage following acute exercise. Medical Science Sports Exercise, 35, 157. Elosua, R., Molina, L., Fito, M., Arquer, A., Sanchez-Quesada, J.L., & Covas, M.I. (2003). Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women. Atherosclerosis, 167, 327-34.
  7. Gackowski, D., Speina, E., Zielinska, M., Kowalewski, J., Rozalski, R., & Siomek, A. (2003). Products of oxidative DNA damage and repair as possible biomarkers of susceptibility to lung cancer. Cancer Research, 63(16), 4899–902.
  8. Gohil, K., Rothfuss, L., Lang, J., & Packer, L. (1987). Effect of exercise training on tissue vitamin E and ubiquinone content. Journal of Applied Physiology, 63, 1638-41.
  9. Gül, M., Demircan, B., Taysi, S., Oztasan, N., Gumustekin, K., & Sıktar, E. (2006). Effects of endurance training and acute exhaustive exercise on antioxidant defense mechanisms in rat heart. Comparative Biochemistry and Physiology-Part A: Molecular & Integrative Physiology, 43, 239-45.
  10. Gündüz, F., Şen