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Journals / Cumhuriyet Science Journal / 2021 / Cilt: 42 - Sayı: 3

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Şifa TÜRKOĞLU
Pages
515–525
Publication Date
2022-10-12
DOI
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⚡ TL;DR(Too Long; Didn't Read)

A significant increase in DNA damage was also observed at all concentrations of both Reactive Blue 19 and Reactive Black 5 examined by comet assay.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the cytotoxic and genotoxic effects of Reactive Blue 19 and Reactive Black 5 were investigated using the Allium test and comet assay.
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Key Findings & Data:Bulbs with roots of Allium cepa L. were treated with different concentrations (25, 50 and 100 ppm) of these textile dyes for 24 h. and 48 h.
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Conclusion & Impact:A significant increase in DNA damage was also observed at all concentrations of both Reactive Blue 19 and Reactive Black 5 examined by comet assay.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the cytotoxic and genotoxic effects of Reactive Blue 19 and Reactive Black 5 were investigated using the Allium test and comet assay. These chemicals are fabric dyes used in textile industries in various parts of Turkey. Bulbs with roots of Allium cepa L. were treated with different concentrations (25, 50 and 100 ppm) of these textile dyes for 24 h. and 48 h. The root tips were processed for cytological studies by the aseto-orcein squash procedure. Distilled water and methyl methane sulfonate (MMS, 10 ppm) were used as an negative and positive control, respectively. Exposure of Reactive Blue 19 and Reactive Black 5 significantly decreased mitotic index values. Additionally, all treatments changed the frequency of mitotic phases when compared with the control groups. These dyes increased chromosome aberrations in test material. Among these abnormalities were anaphase bridges, c-mitosis, laggards, micronuclei and stickiness. A significant increase in DNA damage was also observed at all concentrations of both Reactive Blue 19 and Reactive Black 5 examined by comet assay.

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Cite This Article

TÜRKOĞLU, Ş. (2022). Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator. Cumhuriyet Science Journal, Cilt: 42 - Sayı: 3, 515–525. https://ajindex.com/articles/477529

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References (1)

  1. 1] Ekinci İ., Bir tekstil boyası olan poly r-478’in streptomisetler ile renk giderimi, Yüksek Lisans Tezi, Mersin Üniv., Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Mersin, (2007). [2] Al-Sabti K., Chlorotriazine reactive azo red 120 textile dye induces micronuclei in fish, Ecotoxicol. Environ. Saf., 47 (2000) 149-155. [3] Odeigah P.G.C., Osanyipeju A.O., Genotoxic effects of two industrial effluents and methyl methane sulfonate in Clarias L azera, Food Chem. Toxicol., 33 (1995) 501- 505. [4] Sumathi M., Kalaiselvi K., Palanivel M., Rajaguru P., Genotoxicity of textile dye effluent on fish (Cyprinus carpio) measured using the comet assay, Bull. Environ. Contam. Toxicol., 66 (2001) 407- 414. [5] Çavaş T., Ergene- Gözükara S., Micronuclei, nuclear lesions and interphase silver-stained nucleolar organizer regions (agnors) as cyto- genotoxicity indicators in Oreochromis niloticus exposed to textile mill effluent, Mutation Research., 538 (2003) 81-91. [6] Moawad H., Abd El-Rahim W.M., Khalafallah M.A., Evaluation of biotoxicity of textile dyes using two bioassay tests. J. Basic Microbiol., 43 (3) ( 2003) 218- 229. [7] Fiskesjo G., The Allium test-an alternative in environmental studies: the relative toxicity of metal ions, Mutat Res., 197 (1988) 243–260. [8] Liu T., Zhu L., Wang J., Wang J., Xie, H., The genotoxic and cytotoxic effects of 1-butyl-3- methylimidazolium chloride in soil on Vicia faba seedlings, Journal of Hazardous Materials, 285 (2015) 27–36. [9] Cui J., Zhang R., Wu G.L., Zhu H.M., Yang H., Salicylic acid reduces napropamide toxicity by preventing its accumulation in rapeseed (Brassica napus L.), Arch. Environ. Contam. Toxicol., 59 (2010) 100–108. [10] Liman R., Ciğerci İ.H., Ozturk N.S., Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay, Pesticide Biochemistry and Physiology, 118 (2015) 38-42. [11] Singh M., Das A., Singh D., Maiti P., Shabbir M., Das A., High genotoxicity of shipyard contaminants on Allium cepa and calf thymus DNA, Environmental Chemistry Letters, 12(2) (2014) 321-327. [12] Kwon J.Y., Lee S., Y. Koedrith P., Lee J. Y., Kim K. M., Oh J. M., Yang S. I., Kim M. K., Lee J. K., Jeong J., Maeng E. H., Lee B. J., Seo Y. R., Lack of genotoxic potential of ZnO nanoparticles in in vitro and in vivo tests, Mutation research/Genetic Toxicology and Environmental Mutagenesis, 761 (2014) 1-9. [13] Türkoğlu Ş., Genotoxicity of five food preser