Results, presented as histograms, were the indication of original findings,as most of them were new to the relevant literature.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:Four different pectinolytic enzyme activity essays were employed to detect different pectinases ofBacillus clausii from an extracellular protein cocktail.
📊
Key Findings & Data:The mixture was obtained by ethanol precipitation.Dinitrosalicylic acid (DNS) and sodium hydroxide (NaOH) titrimetric methods were used for thepectinase assays.
💡
Conclusion & Impact:Results, presented as histograms, were the indication of original findings,as most of them were new to the relevant literature.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
Four different pectinolytic enzyme activity essays were employed to detect different pectinases ofBacillus clausii from an extracellular protein cocktail. The mixture was obtained by ethanol precipitation.Dinitrosalicylic acid (DNS) and sodium hydroxide (NaOH) titrimetric methods were used for thepectinase assays. Each of the essays produced a positive result, indicating the presence of pectate lyase(PGL), pectin lyase (PL), pectin methyl esterase (PME), and polygalacturonase (PG). Activities of theseenzymes were then assessed at differing concentrations of NaCl, detergents (SDS and tweens), ionchelators, and organic solvents. Results, presented as histograms, were the indication of original findings,as most of them were new to the relevant literature.
🔖
Cite This Article
Çetinkaya, S., Berber, S. (2022). Effects of Detergents, Ions, and Organic Solvents on the Activity of Four Bacillus clausii Pectinases. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, Cilt: 16 - Sayı: 4, 387–392. https://ajindex.com/articles/472166
McNeil, M., Darvill, A.G., Fry, S.C. and Albersheim, P. 1984. Structure and Function of The Primary Cell Walls of Plants. Annual Review of Biochemistry; 53: 625-663
Abbott, D.W. and Boraston, A.B. 2008. Structural Biology of Pectin Degradation by Enretobacteriaceae. Microbiology and Molecular Biology Reviews; 72: 301-316
Vries, R.P. and Visser, J. 2001. Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides. Microbiology and Molecular Biology Reviews; 65: 497-522
Abbott, D.W. and Boraston, A.B. 2008. Structural Biology of Pectin Degradation by Enretobacteriaceae. Microbiology and Molecular Biology Reviews; 72: 301-316
Khairnar, Y., Krishna, V.K., Boraste, A., Gupta, N., Trivedi, S., Patli, P., Gupta, G., Gupta, M., Jhadav, A., Mujapara, A., Joshi, B. and Mishra, D. 2009. Study of Pectinase Production in Submerged Fermentation Using Different Strains of Aspergillus niger. International Journal of Microbiology Research; 1: 13-17
Hoondal, G.S., Tiwari, R.P., Tewari, R., Dahiya, N. and Beg, Q.K. 2002. Microbial Alkaline Pectinases and Their Industrial Applications: A Review. Applied Microbiology Biotechnology; 59: 409-418
Kashyap, D.R., Vohra, P.K. Chopra, S. and Tewari, R. 2001. Applications of Pectinases in the Commercial Sector: A Review. Bioresource Technology; 77: 215-227
Mojsov, K., 2012. Biotechnological applications of pectinases in textile processing and bioscouring of cotton fibers. II International Conference Industrial Engineering and Environmental Protection Zrenjanin, Serbia, 314-322
Sharma, N., Rathore, M. and Sharma, M. 2012. Microbial Pectinase: Sources, Characterization and Applications. Reviews in Environmental Science and Bio/Technology; 12: 45-60
Berber, S. 2018. Productıon, Isolatıon and Characterızatıon of Extracellular Pectinase from Soil Bacteria. Master's Thesis, Cumhuriyet University Institute of Science; Sivas, 21
Berber, S. and Çetinkaya, S. 2020. An Isolate of Bacillus clausii Appears to Possess Four Distinct Pectinolytic Activities. Iğdır University Journal of the Institute of Science; 10, in press
Kocabay, S. 2015. Producdution, Isolation and Characterization of Extracellular Amylase Enzyme from Gaıta Bacreria. Master's Thesis, Cumhuriyet Üniversitesi Fen Bilimleri Enstitüsü; Sivas, 96
Whitaker, J.R. 1990. Microbial Pectolytic Enzymes. Microbial Enzymes and Biotechnology, Fogarty, W. M. ve Kelly, C. T. (Ed.), Department of Industrial Microbiology, Uni