Journals / Bitlis Eren Üniversitesi Fen Bilimleri Dergisi / 2019 / Cilt: 8 - Sayı: 3

The Efficient Germination Method For Marhsmallows With Medicinal Importance: The Nicking on Seed Coat

Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması

Pages
843–851
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results of the germination index, while the seeds nicked on coat were easily germinated, it was observed that incubation at 42°C did not affect the germination.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The genus Alcea one of the member of Malvaceae family includes some species having medicinal importance.
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Key Findings & Data:For this purpose, three different concentrations (5%, 7.5% and 10%) of the sodium hypochlorite which is a sterilizing agent, 42°C incubation and nicking on the seed coat with blade were tested.
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Conclusion & Impact:According to the results of the germination index, while the seeds nicked on coat were easily germinated, it was observed that incubation at 42°C did not affect the germination.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The genus Alcea one of the member of Malvaceae family includes some species having medicinal importance. Alcea kurdica Alef. (Deve gülü Marshmallow) is a medicinal plant used against tonsils, gastric ulcers, pneumonia, excretory system infections and pelade. The aim of this study was to develope an efficient germination method under sterile conditions and to make the plant suitable for tissue culture studies. For this purpose, three different concentrations (5%, 7.5% and 10%) of the sodium hypochlorite which is a sterilizing agent, 42°C incubation and nicking on the seed coat with blade were tested. While 5% and 7.5% of sodium hypochlorite concentrations were not sufficient for sterilization, 10% of sodium hypochlorite concentration was observed to be sufficient. According to the results of the germination index, while the seeds nicked on coat were easily germinated, it was observed that incubation at 42°C did not affect the germination. On the seventh day, cotyledon, cotyledonary node and hypocotyl tissues were ready to use for tissue culture. On the eighth day, leaves began to form. Leaf, petiole and shoot tip explants were available to use in tissue culture on the thirtieth day. As a result, an efficient germination method for deve gülü marshmallow having medicinal importance was developed with this study

Özet

Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazı türleri içermektedir. Alcea kurdicaAlef. (Deve gülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemi enfeksiyonlarına ve saç kırana karşıkullanılan tıbbi bir bitkidir. Bu çalışmanın amacı, deve gülü hatmi bitkisi için steril şartlar altında etkili birçimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürü çalışmalarında kullanılabilir hale getirilmesidir.Bu amaç için, steril bir ajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°C sıcaklıktainkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesi uygulamaları test edilmiştir. %5 ve %7.5 sodyumhipoklorit konsantrasyonlarının sterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipokloritkonsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme oranları sonuçlarına göre, kabuğu çentiklenentohumlar hızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir. Yedinci günde,kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürü için kullanılabilir duruma gelmiştir. Sekizinci günde,yapraklar oluşmaya başlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu günde dokukültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma ile tıbbi öneme sahip deve gülü hatmi içinetkili bir çimlendirme yöntemi geliştirilmiştir.

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Cite This Article

TÜRKER, M., BATTAL, A., GÖRMEZ, G. (2022). The Efficient Germination Method For Marhsmallows With Medicinal Importance: The Nicking on Seed Coat. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, Cilt: 8 - Sayı: 3, 843–851. https://ajindex.com/articles/467116

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